Source water microorganism assessment in three cities in China: A comparative study
Reservoirs, rivers and groundwater are the top three sources of drinking water supplies in China. As microbial contamination of drinking water is still a prominent water quality problem in rural areas, understanding the microbial quality of these sources is important to the public’s health and econo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2022.990104/full |
| _version_ | 1811231298039054336 |
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| author | Yang Liu Yang Liu Charlotte D. Smith Hongxing Li Duochun Wang |
| author_facet | Yang Liu Yang Liu Charlotte D. Smith Hongxing Li Duochun Wang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Reservoirs, rivers and groundwater are the top three sources of drinking water supplies in China. As microbial contamination of drinking water is still a prominent water quality problem in rural areas, understanding the microbial quality of these sources is important to the public’s health and economic prosperity of communities. In this study, three types of source water samples were collected from three cities in China. Bacterial contamination indicators testing showed that: total coliforms (TCs) and potential E. coli were not detected in groundwater, but both were detected in river and reservoir water. Total bacteria (TB) of rivers and Res-Ⅰ (sampling site Ⅰ of reservoir water) were greater than 100 CFU/ml, while less than 100 CFU/ml from Res-Ⅱ (sampling site Ⅱ of reservoir water) and groundwater. Salmonella spp. were isolated from river water and no pathogenic microorganisms were isolated from the other two types of water sources by selective culture. Microbial communities testing by 16S rRNA gene amplicon sequencing indicated that, there were 14,114 operational taxonomic unit (OTU) of microbial abundance from all 30 samples, and most OTUs were only present in river water (15.17%), reservoir water (10.46%) or groundwater (43.91%), while 1540 OTUs (10.91%) were shared by all three types of water sources. There were significant differences in the microbial communities of the three types of source water (p < 0.05). Based on the Ace, Chao, and Shannon-Weaver, and Simpson indexes, the species diversity of bacteria in groundwater was higher than in river water or reservoir water (p < 0.05), with the reservoir water having the lowest diversity of bacteria. More than seven potential pathogenic bacteria were detected in 30 water samples, for example, E. coli, Staphylococcus aureus, Clostridioides difficile and Bacteroides fragilis were present in all three types of water sources, while other pathogenic bacteria occurred only in some of the water samples. Clostridium perfringens were detected in river water and groundwater. This study adds information on the microbial communities of various drinking water sources in rural China, which is valuable to water treatment and waterborne pathogen studies. In addition, this study supports the idea that 16S rRNA gene amplicon sequencing could be used as a supplementary tool for sources water quality monitoring. |
| first_indexed | 2024-04-12T10:42:17Z |
| format | Article |
| id | doaj.art-2be5abb0d0484941ae1de7e2af64c970 |
| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-04-12T10:42:17Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Environmental Science |
| spelling | doaj.art-2be5abb0d0484941ae1de7e2af64c9702022-12-22T03:36:33ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2022-11-011010.3389/fenvs.2022.990104990104Source water microorganism assessment in three cities in China: A comparative studyYang Liu0Yang Liu1Charlotte D. Smith2Hongxing Li3Duochun Wang4National Center for Rural Water Supply Technical Guidance, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing, ChinaSchool of Public Health, University of California, Berkeley, Berkeley, CA, United StatesNational Center for Rural Water Supply Technical Guidance, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Beijing, ChinaReservoirs, rivers and groundwater are the top three sources of drinking water supplies in China. As microbial contamination of drinking water is still a prominent water quality problem in rural areas, understanding the microbial quality of these sources is important to the public’s health and economic prosperity of communities. In this study, three types of source water samples were collected from three cities in China. Bacterial contamination indicators testing showed that: total coliforms (TCs) and potential E. coli were not detected in groundwater, but both were detected in river and reservoir water. Total bacteria (TB) of rivers and Res-Ⅰ (sampling site Ⅰ of reservoir water) were greater than 100 CFU/ml, while less than 100 CFU/ml from Res-Ⅱ (sampling site Ⅱ of reservoir water) and groundwater. Salmonella spp. were isolated from river water and no pathogenic microorganisms were isolated from the other two types of water sources by selective culture. Microbial communities testing by 16S rRNA gene amplicon sequencing indicated that, there were 14,114 operational taxonomic unit (OTU) of microbial abundance from all 30 samples, and most OTUs were only present in river water (15.17%), reservoir water (10.46%) or groundwater (43.91%), while 1540 OTUs (10.91%) were shared by all three types of water sources. There were significant differences in the microbial communities of the three types of source water (p < 0.05). Based on the Ace, Chao, and Shannon-Weaver, and Simpson indexes, the species diversity of bacteria in groundwater was higher than in river water or reservoir water (p < 0.05), with the reservoir water having the lowest diversity of bacteria. More than seven potential pathogenic bacteria were detected in 30 water samples, for example, E. coli, Staphylococcus aureus, Clostridioides difficile and Bacteroides fragilis were present in all three types of water sources, while other pathogenic bacteria occurred only in some of the water samples. Clostridium perfringens were detected in river water and groundwater. This study adds information on the microbial communities of various drinking water sources in rural China, which is valuable to water treatment and waterborne pathogen studies. In addition, this study supports the idea that 16S rRNA gene amplicon sequencing could be used as a supplementary tool for sources water quality monitoring.https://www.frontiersin.org/articles/10.3389/fenvs.2022.990104/fullmicrobial characteristicswater sourcesindicator microorganisms16S rRNA sequencingmicrobial water qualitymonitoring |
| spellingShingle | Yang Liu Yang Liu Charlotte D. Smith Hongxing Li Duochun Wang Source water microorganism assessment in three cities in China: A comparative study Frontiers in Environmental Science microbial characteristics water sources indicator microorganisms 16S rRNA sequencing microbial water quality monitoring |
| title | Source water microorganism assessment in three cities in China: A comparative study |
| title_full | Source water microorganism assessment in three cities in China: A comparative study |
| title_fullStr | Source water microorganism assessment in three cities in China: A comparative study |
| title_full_unstemmed | Source water microorganism assessment in three cities in China: A comparative study |
| title_short | Source water microorganism assessment in three cities in China: A comparative study |
| title_sort | source water microorganism assessment in three cities in china a comparative study |
| topic | microbial characteristics water sources indicator microorganisms 16S rRNA sequencing microbial water quality monitoring |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2022.990104/full |
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