Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model
<i>Background and Objectives</i>: The most common kidney stones are calcium stones and calcium oxalate (CaOx) stones are the most common type of calcium stones. Hyperoxaluria is an essential risk factor for the formation of these stones. Quercetin is a polyphenol with antioxidant, anti-i...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/1648-9144/57/6/566 |
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| author | Ahmet Guzel Sedat Yunusoglu Mustafa Calapoglu Ibrahim Aydın Candan Ibrahim Onaran Meral Oncu Osman Ergun Taylan Oksay |
| author_facet | Ahmet Guzel Sedat Yunusoglu Mustafa Calapoglu Ibrahim Aydın Candan Ibrahim Onaran Meral Oncu Osman Ergun Taylan Oksay |
| author_sort | Ahmet Guzel |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The most common kidney stones are calcium stones and calcium oxalate (CaOx) stones are the most common type of calcium stones. Hyperoxaluria is an essential risk factor for the formation of these stones. Quercetin is a polyphenol with antioxidant, anti-inflammatory, and many other physiological effects. The aim of this study was to investigate the protective effect of quercetin in hyperoxaluria-induced nephrolithiasis. <i>Materials and Methods</i>: Male Wistar-Albino rats weighing 250–300 g (<i>n</i> = 24) were randomized into three groups: Control (<i>n</i> = 8), ethylene glycol (EG) (<i>n</i> = 8), and EG + quercetin (<i>n</i> = 8). One percent EG-water solution was given to all rats except for the control group as drinking water for five weeks. Quercetin-water solution was given to the EG + quercetin group by oral gavage at a dose of 10 mg/kg/day. Malondialdehyde (MDA), catalase (CAT), urea, calcium, and oxalate levels were analyzed in blood and urine samples. Histopathological assessments and immunohistochemical analyses for oxidative stress and inflammation indicators p38 mitogen-activated protein kinase (p38-MAPK) and nuclear factor kappa B (NF-kB) were performed on renal tissues. <i>Results</i>: The MDA levels were significantly lower in the quercetin-treated group than in the EG-treated group (<i>p</i> = 0.001). Although CAT levels were higher in the quercetin-treated group than the EG-administered group, they were not significantly different between these groups. The expression of p38 MAPK was significantly less in the quercetin-treated group than the EG group (<i>p</i> < 0.004). There was no statistically significant difference between the quercetin and EG groups in terms of NF-kB expression. <i>Conclusions</i>: We conclude that hyperoxaluria activated the signaling pathways, which facilitate the oxidative processes leading to oxalate stone formation in the kidneys. Our findings indicated that quercetin reduced damage due to hyperoxaluria. These results imply that quercetin can be considered a therapeutic agent for decreasing oxalate stone formation, especially in patients with recurrent stones due to hyperoxaluria. |
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| last_indexed | 2024-03-10T10:45:39Z |
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| spelling | doaj.art-2be6d43e9b8b419d819274d7e37e20382023-11-21T22:36:57ZengMDPI AGMedicina1010-660X1648-91442021-06-0157656610.3390/medicina57060566Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria ModelAhmet Guzel0Sedat Yunusoglu1Mustafa Calapoglu2Ibrahim Aydın Candan3Ibrahim Onaran4Meral Oncu5Osman Ergun6Taylan Oksay7Department of Urology, Aydın State Hospital, Aydın 09100, TurkeyDepartment of Urology, Afyonkarahisar State Hospital, Afyonkarahisar 03100, TurkeyDepartment of Biochemistry, Faculty of Arts and Science, Suleyman Demirel University, Isparta 32100, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya 07100, TurkeyDepartment of Medical Biology, Faculty of Medicine, Suleyman Demirel University, Isparta 32100, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Suleyman Demirel University, Isparta 32100, TurkeyDepartment of Urology, Faculty of Medicine, Suleyman Demirel University, Isparta 32100, TurkeyDepartment of Urology, Faculty of Medicine, Suleyman Demirel University, Isparta 32100, Turkey<i>Background and Objectives</i>: The most common kidney stones are calcium stones and calcium oxalate (CaOx) stones are the most common type of calcium stones. Hyperoxaluria is an essential risk factor for the formation of these stones. Quercetin is a polyphenol with antioxidant, anti-inflammatory, and many other physiological effects. The aim of this study was to investigate the protective effect of quercetin in hyperoxaluria-induced nephrolithiasis. <i>Materials and Methods</i>: Male Wistar-Albino rats weighing 250–300 g (<i>n</i> = 24) were randomized into three groups: Control (<i>n</i> = 8), ethylene glycol (EG) (<i>n</i> = 8), and EG + quercetin (<i>n</i> = 8). One percent EG-water solution was given to all rats except for the control group as drinking water for five weeks. Quercetin-water solution was given to the EG + quercetin group by oral gavage at a dose of 10 mg/kg/day. Malondialdehyde (MDA), catalase (CAT), urea, calcium, and oxalate levels were analyzed in blood and urine samples. Histopathological assessments and immunohistochemical analyses for oxidative stress and inflammation indicators p38 mitogen-activated protein kinase (p38-MAPK) and nuclear factor kappa B (NF-kB) were performed on renal tissues. <i>Results</i>: The MDA levels were significantly lower in the quercetin-treated group than in the EG-treated group (<i>p</i> = 0.001). Although CAT levels were higher in the quercetin-treated group than the EG-administered group, they were not significantly different between these groups. The expression of p38 MAPK was significantly less in the quercetin-treated group than the EG group (<i>p</i> < 0.004). There was no statistically significant difference between the quercetin and EG groups in terms of NF-kB expression. <i>Conclusions</i>: We conclude that hyperoxaluria activated the signaling pathways, which facilitate the oxidative processes leading to oxalate stone formation in the kidneys. Our findings indicated that quercetin reduced damage due to hyperoxaluria. These results imply that quercetin can be considered a therapeutic agent for decreasing oxalate stone formation, especially in patients with recurrent stones due to hyperoxaluria.https://www.mdpi.com/1648-9144/57/6/566calcium oxalatehyperoxaluriaoxidative stressquercetin |
| spellingShingle | Ahmet Guzel Sedat Yunusoglu Mustafa Calapoglu Ibrahim Aydın Candan Ibrahim Onaran Meral Oncu Osman Ergun Taylan Oksay Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model Medicina calcium oxalate hyperoxaluria oxidative stress quercetin |
| title | Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model |
| title_full | Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model |
| title_fullStr | Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model |
| title_full_unstemmed | Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model |
| title_short | Protective Effects of Quercetin on Oxidative Stress-Induced Tubular Epithelial Damage in the Experimental Rat Hyperoxaluria Model |
| title_sort | protective effects of quercetin on oxidative stress induced tubular epithelial damage in the experimental rat hyperoxaluria model |
| topic | calcium oxalate hyperoxaluria oxidative stress quercetin |
| url | https://www.mdpi.com/1648-9144/57/6/566 |
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