Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran
Background and Objectives: Diarrheagenic Escherichia coli (DEC) is an emerging agent among pathogens that causes diarrhea. Studies showed that diarrheagenic E. coli such as enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (E...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-03-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/1165 |
| _version_ | 1818915794468208640 |
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| author | Atieh Darbandi Parviz Owlia Saeid Bouzari Horieh Saderi |
| author_facet | Atieh Darbandi Parviz Owlia Saeid Bouzari Horieh Saderi |
| author_sort | Atieh Darbandi |
| collection | DOAJ |
| description | Background and Objectives: Diarrheagenic Escherichia coli (DEC) is an emerging agent among pathogens that causes diarrhea. Studies showed that diarrheagenic E. coli such as enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), diffusely adhering E. coli (DAEC) and shiga toxin producing E. coli (STEC) strains are among the most frequent causative agents in acute diarrhea. The aim of this study was to determine the frequency of DEC pathotypes in Khuzestan province.
Materials and Methods: Stool samples were collected from patients with diarrhea in Khuzestan province of Iran. E. coli strains were isolated using conventional culture and standard biochemical tests. The polymerase chain reaction (PCR) technique was used to detect presence of virulence genes, i.e; eae, stx1 and stx2 for EHEC, bfp and eae for EPEC, LT and ST for ETEC, AA for EAEC, invE for EIEC, stx1 and stx2 for STEC.
Results: Altogether, 200 stool samples were obtained from patients, of which 158 (79%) were positive for E. coli. DEC was identified in 127 (63%) of stool samples, which frequency of each pathotypes were as follows: atypical EPEC 49 (39%), typical EPEC 1 (0.7%), STEC 50 (39.3%), ETEC 21 (16.3%), EAEC 5 (4.0%) and EIEC 1 (0.7%). Most frequent etiological agents of diarrhea in Khuzestan province of Iran were STEC and EPEC.
Conclusion: Our findings showed DEC had been agent of diarrhea in Khuzestan. This finding provides evidence that effort should be made to estimate the burden of infection by the etiological agent for better medical approach and should raise notification about antibiotic resistance among bacterial infection. |
| first_indexed | 2024-12-20T00:07:56Z |
| format | Article |
| id | doaj.art-2be9d6c2c8414a679a6e8375018ea3ea |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-20T00:07:56Z |
| publishDate | 2017-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-2be9d6c2c8414a679a6e8375018ea3ea2022-12-21T20:00:35ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472017-03-0186Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of IranAtieh Darbandi0Parviz Owlia1Saeid Bouzari2Horieh Saderi3Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, IranMolecular Microbiology Research Center, Shahed University, Tehran, IranMolecular Biology Department, Pasteur Institute of Iran, Tehran, IranMolecular Microbiology Research Center, Shahed University, Tehran, IranBackground and Objectives: Diarrheagenic Escherichia coli (DEC) is an emerging agent among pathogens that causes diarrhea. Studies showed that diarrheagenic E. coli such as enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), diffusely adhering E. coli (DAEC) and shiga toxin producing E. coli (STEC) strains are among the most frequent causative agents in acute diarrhea. The aim of this study was to determine the frequency of DEC pathotypes in Khuzestan province. Materials and Methods: Stool samples were collected from patients with diarrhea in Khuzestan province of Iran. E. coli strains were isolated using conventional culture and standard biochemical tests. The polymerase chain reaction (PCR) technique was used to detect presence of virulence genes, i.e; eae, stx1 and stx2 for EHEC, bfp and eae for EPEC, LT and ST for ETEC, AA for EAEC, invE for EIEC, stx1 and stx2 for STEC. Results: Altogether, 200 stool samples were obtained from patients, of which 158 (79%) were positive for E. coli. DEC was identified in 127 (63%) of stool samples, which frequency of each pathotypes were as follows: atypical EPEC 49 (39%), typical EPEC 1 (0.7%), STEC 50 (39.3%), ETEC 21 (16.3%), EAEC 5 (4.0%) and EIEC 1 (0.7%). Most frequent etiological agents of diarrhea in Khuzestan province of Iran were STEC and EPEC. Conclusion: Our findings showed DEC had been agent of diarrhea in Khuzestan. This finding provides evidence that effort should be made to estimate the burden of infection by the etiological agent for better medical approach and should raise notification about antibiotic resistance among bacterial infection.https://ijm.tums.ac.ir/index.php/ijm/article/view/1165Escherichia colipathotypeDiarrhea |
| spellingShingle | Atieh Darbandi Parviz Owlia Saeid Bouzari Horieh Saderi Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran Iranian Journal of Microbiology Escherichia coli pathotype Diarrhea |
| title | Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran |
| title_full | Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran |
| title_fullStr | Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran |
| title_full_unstemmed | Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran |
| title_short | Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran |
| title_sort | diarrheagenic escherichia coli pathotypes frequency in khuzestan province of iran |
| topic | Escherichia coli pathotype Diarrhea |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/1165 |
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