Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis
Background: Cystic fibrosis (CF) is an autosomal recessive disorder due to genetic defect resulting cilliary function impairment. So that these patients are inclined to recurrent infection with opportunistic microorganism such as Pseudomonas aeruginosa. This organism is most important pathogen in cy...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2012-03-01
|
| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/1481 |
| _version_ | 1797708376524718080 |
|---|---|
| author | Hossein Fazeli Reza Akbari Sharaeh Moghim Asadollah Asadian Jamal Faghihinia Hossein Saneeyan Tahmineh Narimani |
| author_facet | Hossein Fazeli Reza Akbari Sharaeh Moghim Asadollah Asadian Jamal Faghihinia Hossein Saneeyan Tahmineh Narimani |
| author_sort | Hossein Fazeli |
| collection | DOAJ |
| description | Background: Cystic fibrosis (CF) is an autosomal recessive disorder due to genetic defect resulting cilliary function impairment. So that these patients are inclined to recurrent infection with opportunistic microorganism such as Pseudomonas aeruginosa. This organism is most important pathogen in cystic fibrosis (CF) which intend to colonize and form biofilm to confront immunsystem and therapeutic dosage of antimicrobial drug. These bacteria can mutate to survive and adapted to new to new condition. Colony morphotyping and antimicrobial drug resistant detecting in Pseudomonas aeruginosa can help treat of CF patient and prevent patient form being resource of resistant strain bacteria. The aim of this assay is detection of colony morphotype, antimicrobial resistant in Pseudomonas aeruginosa isolated form CF and urinary tract infection (UTI) patient, hospital personnel and environment.
Methods: It is a cross sectional study. Samples wet were taken with swab in 59 CF patients (between 82-87 years), 27 UTI patient, 113 sample from hospital environment, 123 sample from 91 personnel (throat, hand, lab uniform) in Alzahra Hospital, Isfahan, Iran. Isolated Pseudomonas aeruginosa form these samples were identified bacteriologic standard method. The isolates were investigated for morphotyping and antimicrobial drug resistance.
Findings: 21 cases form 59 patients (35.5%), 25 cases 27 UTI patient (92.5%) were infected with Pseudomonas aeruginosa. Pseudomonas aeruginosa were isolated from 11 of 113 sample taken from hospital environment and 4 of the (123 sample) were taken 91 hospital personnel. From 21 Pseudomonas aeruginosa isolated from CF patient (35 male, 24 female and the ages between 2 month to 24 years), 9 isolates had mucoid form (42%) and the rate antibiotic resistance to Gentamicin, Amikacin, ciprofloxacin, piperacillin and ceftazidime were 9.5%, 9.5%, 14.2%, 19% and 86% respectively.
Conclusion: The mucoid form of Pseudomonas aeruginosa was seen most frequently in CF patients comparing with other samples. In all groups, antimicrobial resistance to Ceftazidime and antimicrobial sensitivity to Amikacin were most frequent. |
| first_indexed | 2024-03-12T06:21:32Z |
| format | Article |
| id | doaj.art-2be9fff6e05941f8adafa81eefdde0b4 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T06:21:32Z |
| publishDate | 2012-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-2be9fff6e05941f8adafa81eefdde0b42023-09-03T02:11:06ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-03-0129171877Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic FibrosisHossein Fazeli0Reza Akbari1Sharaeh Moghim2Asadollah Asadian3Jamal Faghihinia4Hossein Saneeyan5Tahmineh Narimani6Assistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranMSc Student, Department of Microbiology, School of Medicine And Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Pediatrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Pediatrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Cystic fibrosis (CF) is an autosomal recessive disorder due to genetic defect resulting cilliary function impairment. So that these patients are inclined to recurrent infection with opportunistic microorganism such as Pseudomonas aeruginosa. This organism is most important pathogen in cystic fibrosis (CF) which intend to colonize and form biofilm to confront immunsystem and therapeutic dosage of antimicrobial drug. These bacteria can mutate to survive and adapted to new to new condition. Colony morphotyping and antimicrobial drug resistant detecting in Pseudomonas aeruginosa can help treat of CF patient and prevent patient form being resource of resistant strain bacteria. The aim of this assay is detection of colony morphotype, antimicrobial resistant in Pseudomonas aeruginosa isolated form CF and urinary tract infection (UTI) patient, hospital personnel and environment. Methods: It is a cross sectional study. Samples wet were taken with swab in 59 CF patients (between 82-87 years), 27 UTI patient, 113 sample from hospital environment, 123 sample from 91 personnel (throat, hand, lab uniform) in Alzahra Hospital, Isfahan, Iran. Isolated Pseudomonas aeruginosa form these samples were identified bacteriologic standard method. The isolates were investigated for morphotyping and antimicrobial drug resistance. Findings: 21 cases form 59 patients (35.5%), 25 cases 27 UTI patient (92.5%) were infected with Pseudomonas aeruginosa. Pseudomonas aeruginosa were isolated from 11 of 113 sample taken from hospital environment and 4 of the (123 sample) were taken 91 hospital personnel. From 21 Pseudomonas aeruginosa isolated from CF patient (35 male, 24 female and the ages between 2 month to 24 years), 9 isolates had mucoid form (42%) and the rate antibiotic resistance to Gentamicin, Amikacin, ciprofloxacin, piperacillin and ceftazidime were 9.5%, 9.5%, 14.2%, 19% and 86% respectively. Conclusion: The mucoid form of Pseudomonas aeruginosa was seen most frequently in CF patients comparing with other samples. In all groups, antimicrobial resistance to Ceftazidime and antimicrobial sensitivity to Amikacin were most frequent.http://jims.mui.ac.ir/index.php/jims/article/view/1481Cystic fibrosisPseudomonas aeruginosaMorphotyping |
| spellingShingle | Hossein Fazeli Reza Akbari Sharaeh Moghim Asadollah Asadian Jamal Faghihinia Hossein Saneeyan Tahmineh Narimani Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis مجله دانشکده پزشکی اصفهان Cystic fibrosis Pseudomonas aeruginosa Morphotyping |
| title | Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis |
| title_full | Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis |
| title_fullStr | Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis |
| title_full_unstemmed | Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis |
| title_short | Detection of Morphotyping Characteristics Identification Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Cystic Fibrosis |
| title_sort | detection of morphotyping characteristics identification antibiotic resistance of pseudomonas aeruginosa isolated from patients with cystic fibrosis |
| topic | Cystic fibrosis Pseudomonas aeruginosa Morphotyping |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/1481 |
| work_keys_str_mv | AT hosseinfazeli detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis AT rezaakbari detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis AT sharaehmoghim detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis AT asadollahasadian detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis AT jamalfaghihinia detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis AT hosseinsaneeyan detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis AT tahminehnarimani detectionofmorphotypingcharacteristicsidentificationantibioticresistanceofpseudomonasaeruginosaisolatedfrompatientswithcysticfibrosis |