Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages
Human norovirus (HNV) is one of the emerging and rapidly spreading groups of pathogens and the main cause of epidemic viral gastroenteritis globally. Due to a lack of in vitro culture systems and suitable animal models for HNV infection, murine norovirus (MNV) has become a common model. A recent stu...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/1/164 |
| _version_ | 1797436529398775808 |
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| author | Ming Fu Nini Chen Yanhe Zhou Sidong Chen Wanfu Xu Sitang Gong Lanlan Geng |
| author_facet | Ming Fu Nini Chen Yanhe Zhou Sidong Chen Wanfu Xu Sitang Gong Lanlan Geng |
| author_sort | Ming Fu |
| collection | DOAJ |
| description | Human norovirus (HNV) is one of the emerging and rapidly spreading groups of pathogens and the main cause of epidemic viral gastroenteritis globally. Due to a lack of in vitro culture systems and suitable animal models for HNV infection, murine norovirus (MNV) has become a common model. A recent study showed that MNV activates NLRP3 inflammasome leading to pyroptosis. Jatrorrhizine (JAT) is a natural isoquinoline alkaloid isolated from <i>Coptis Chinensis</i>, which has been proven to have antibacterial, anti-inflammatory, and antitumor effects. However, whether JAT has an effect on norovirus gastroenteritis and the underlying molecular mechanism remain unclear. Here, we found that JAT could ameliorate NLRP3-N-GSDMD-dependent pyroptosis induced by MNV infection through inhibiting the MAPKs/NF-κB signaling pathways and decrease MNV replication in RAW264.7 macrophages, suggesting that JAT has the potential to be a therapeutic agent for treating norovirus gastroenteritis. |
| first_indexed | 2024-03-09T11:03:56Z |
| format | Article |
| id | doaj.art-2beb169e25464082a2efecf15036b029 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T11:03:56Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-2beb169e25464082a2efecf15036b0292023-12-01T01:04:08ZengMDPI AGVaccines2076-393X2023-01-0111116410.3390/vaccines11010164Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 MacrophagesMing Fu0Nini Chen1Yanhe Zhou2Sidong Chen3Wanfu Xu4Sitang Gong5Lanlan Geng6Department of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaDepartment of Gastroenterology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaHuman norovirus (HNV) is one of the emerging and rapidly spreading groups of pathogens and the main cause of epidemic viral gastroenteritis globally. Due to a lack of in vitro culture systems and suitable animal models for HNV infection, murine norovirus (MNV) has become a common model. A recent study showed that MNV activates NLRP3 inflammasome leading to pyroptosis. Jatrorrhizine (JAT) is a natural isoquinoline alkaloid isolated from <i>Coptis Chinensis</i>, which has been proven to have antibacterial, anti-inflammatory, and antitumor effects. However, whether JAT has an effect on norovirus gastroenteritis and the underlying molecular mechanism remain unclear. Here, we found that JAT could ameliorate NLRP3-N-GSDMD-dependent pyroptosis induced by MNV infection through inhibiting the MAPKs/NF-κB signaling pathways and decrease MNV replication in RAW264.7 macrophages, suggesting that JAT has the potential to be a therapeutic agent for treating norovirus gastroenteritis.https://www.mdpi.com/2076-393X/11/1/164MNVjatrorrhizineNLRP3inflammasomepyroptosis |
| spellingShingle | Ming Fu Nini Chen Yanhe Zhou Sidong Chen Wanfu Xu Sitang Gong Lanlan Geng Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages Vaccines MNV jatrorrhizine NLRP3 inflammasome pyroptosis |
| title | Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |
| title_full | Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |
| title_fullStr | Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |
| title_full_unstemmed | Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |
| title_short | Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |
| title_sort | jatrorrhizine suppresses murine norovirus triggered n gsdmd dependent pyroptosis in raw264 7 macrophages |
| topic | MNV jatrorrhizine NLRP3 inflammasome pyroptosis |
| url | https://www.mdpi.com/2076-393X/11/1/164 |
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