HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney
We previously demonstrated that mast cells expressing HLA-G are associated with regions of hepatitis C virus-induced liver fibrosis. Here, we aimed to determine whether HLA-G expression in mast cells is specific to viral etiology, the liver, or to the general process of fibrosis. We enumerated HLA-G...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12490 |
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| author | Nicolas Mouchet Nicolas Vu Bruno Turlin Nathalie Rioux-Leclercq Stéphane Jouneau Michel Samson Laurence Amiot |
| author_facet | Nicolas Mouchet Nicolas Vu Bruno Turlin Nathalie Rioux-Leclercq Stéphane Jouneau Michel Samson Laurence Amiot |
| author_sort | Nicolas Mouchet |
| collection | DOAJ |
| description | We previously demonstrated that mast cells expressing HLA-G are associated with regions of hepatitis C virus-induced liver fibrosis. Here, we aimed to determine whether HLA-G expression in mast cells is specific to viral etiology, the liver, or to the general process of fibrosis. We enumerated HLA-G<sup>+</sup> cells and mast cells by the immunohistochemistry of (i) liver blocks from 41 cases of alcoholic cirrhosis, (ii) 10 of idiopathic pulmonary fibrosis (IPF), and (iii) 10 of renal fibrosis. The nature of the HLA-G<sup>+</sup> cells was specified by multiplex immunofluorescence using software. More than half of all HLA-G<sup>+</sup> cells were mast cells in fibrotic areas of alcoholic cirrhosis and IPF. In the kidneys, subjected to fibrosis, the HLA-G<sup>+</sup> cells were indeed mast cells but could not be counted. Moreover, in certain cases of the liver and lung, we observed a number of cellular nodes, which were secondary or tertiary follicles, in which HLA-G was highly expressed by B lymphocytes. In conclusion, HLA-G<sup>+</sup> mast cells could be observed in the fibrotic regions of all organs studied. Previous studies suggest a protective role for HLA-G<sup>+</sup> mast cells against inflammation and fibrosis. The observed follicles with B lymphocytes that express HLA-G may also reinforce their antifibrotic role. |
| first_indexed | 2024-03-10T05:25:22Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:25:22Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2bee05bc1fdd400cb57808d3d2faf44b2023-11-22T23:43:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221249010.3390/ijms222212490HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and KidneyNicolas Mouchet0Nicolas Vu1Bruno Turlin2Nathalie Rioux-Leclercq3Stéphane Jouneau4Michel Samson5Laurence Amiot6Univ Rennes, INSERM, CNRS, Biosit, Core Facility H2P2, F-35000 Rennes, FranceUniv Rennes, INSERM, EHESP, IRSET (Institut de Recherche en Santé, Environnement et Travail)-UMR_S 1085, F-35000 Rennes, FranceUniv Rennes, CHU Rennes, F-35000 Rennes, FranceUniv Rennes, CHU Rennes, INSERM, EHESP, IRSET (Institut de Recherche en Santé, Environnement et Travail)-UMR_S 1085, F-35000 Rennes, FranceUniv Rennes, CHU Rennes, INSERM, EHESP, IRSET (Institut de Recherche en Santé, Environnement et Travail)-UMR_S 1085, F-35000 Rennes, FranceUniv Rennes, INSERM, EHESP, IRSET (Institut de Recherche en Santé, Environnement et Travail)-UMR_S 1085, F-35000 Rennes, FranceUniv Rennes, CHU Rennes, INSERM, EHESP, IRSET (Institut de Recherche en Santé, Environnement et Travail)-UMR_S 1085, F-35000 Rennes, FranceWe previously demonstrated that mast cells expressing HLA-G are associated with regions of hepatitis C virus-induced liver fibrosis. Here, we aimed to determine whether HLA-G expression in mast cells is specific to viral etiology, the liver, or to the general process of fibrosis. We enumerated HLA-G<sup>+</sup> cells and mast cells by the immunohistochemistry of (i) liver blocks from 41 cases of alcoholic cirrhosis, (ii) 10 of idiopathic pulmonary fibrosis (IPF), and (iii) 10 of renal fibrosis. The nature of the HLA-G<sup>+</sup> cells was specified by multiplex immunofluorescence using software. More than half of all HLA-G<sup>+</sup> cells were mast cells in fibrotic areas of alcoholic cirrhosis and IPF. In the kidneys, subjected to fibrosis, the HLA-G<sup>+</sup> cells were indeed mast cells but could not be counted. Moreover, in certain cases of the liver and lung, we observed a number of cellular nodes, which were secondary or tertiary follicles, in which HLA-G was highly expressed by B lymphocytes. In conclusion, HLA-G<sup>+</sup> mast cells could be observed in the fibrotic regions of all organs studied. Previous studies suggest a protective role for HLA-G<sup>+</sup> mast cells against inflammation and fibrosis. The observed follicles with B lymphocytes that express HLA-G may also reinforce their antifibrotic role.https://www.mdpi.com/1422-0067/22/22/12490fibrosisHLA-Gmast cellsfolliclesB lymphocytesantifibrotic |
| spellingShingle | Nicolas Mouchet Nicolas Vu Bruno Turlin Nathalie Rioux-Leclercq Stéphane Jouneau Michel Samson Laurence Amiot HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney International Journal of Molecular Sciences fibrosis HLA-G mast cells follicles B lymphocytes antifibrotic |
| title | HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney |
| title_full | HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney |
| title_fullStr | HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney |
| title_full_unstemmed | HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney |
| title_short | HLA-G Is Widely Expressed by Mast Cells in Regions of Organ Fibrosis in the Liver, Lung and Kidney |
| title_sort | hla g is widely expressed by mast cells in regions of organ fibrosis in the liver lung and kidney |
| topic | fibrosis HLA-G mast cells follicles B lymphocytes antifibrotic |
| url | https://www.mdpi.com/1422-0067/22/22/12490 |
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