An immunochromatographic strip sensor for marbofloxacin residues.
Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. Th...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0299709&type=printable |
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| author | Xingdong Yang Qingmei Li Sharon Kwee Jifei Yang Qianqian Zhang Xiaofei Hu |
| author_facet | Xingdong Yang Qingmei Li Sharon Kwee Jifei Yang Qianqian Zhang Xiaofei Hu |
| author_sort | Xingdong Yang |
| collection | DOAJ |
| description | Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC50) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC‒MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner. |
| first_indexed | 2024-04-24T13:46:54Z |
| format | Article |
| id | doaj.art-2bef5d1822e04df7bb7f9578aa111334 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-24T13:46:54Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2bef5d1822e04df7bb7f9578aa1113342024-04-04T05:34:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01193e029970910.1371/journal.pone.0299709An immunochromatographic strip sensor for marbofloxacin residues.Xingdong YangQingmei LiSharon KweeJifei YangQianqian ZhangXiaofei HuMarbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC50) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC‒MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0299709&type=printable |
| spellingShingle | Xingdong Yang Qingmei Li Sharon Kwee Jifei Yang Qianqian Zhang Xiaofei Hu An immunochromatographic strip sensor for marbofloxacin residues. PLoS ONE |
| title | An immunochromatographic strip sensor for marbofloxacin residues. |
| title_full | An immunochromatographic strip sensor for marbofloxacin residues. |
| title_fullStr | An immunochromatographic strip sensor for marbofloxacin residues. |
| title_full_unstemmed | An immunochromatographic strip sensor for marbofloxacin residues. |
| title_short | An immunochromatographic strip sensor for marbofloxacin residues. |
| title_sort | immunochromatographic strip sensor for marbofloxacin residues |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0299709&type=printable |
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