Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil
A significant difference in the seed germinability was observed between the two rice cultivars, ‘Nipponbare’ and ‘Suweon 287’, under anoxia (i.e., during germination in submerged soil at 18°C), although little difference was seen under aerobic (in air) or hypoxic (in water) conditions. The number of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2000-01-01
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| Series: | Plant Production Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1626/pps.3.32 |
| _version_ | 1828472402157240320 |
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| author | Jirong Huang Kyoko Toyofuku Junji Yamaguchi Shigemi Akita |
| author_facet | Jirong Huang Kyoko Toyofuku Junji Yamaguchi Shigemi Akita |
| author_sort | Jirong Huang |
| collection | DOAJ |
| description | A significant difference in the seed germinability was observed between the two rice cultivars, ‘Nipponbare’ and ‘Suweon 287’, under anoxia (i.e., during germination in submerged soil at 18°C), although little difference was seen under aerobic (in air) or hypoxic (in water) conditions. The number of α-amylase isoforms synthesized in germinating seeds was inversely proportional to the O2 concentrations at the early germination stage. The formation of isoform B was promoted by oxygen supply, while isoform H was undetectable if the seeds were unable to germinate. The activity of isoform H was highly correlated with the coleoptile length in the submerged soil at 18°C, indicating that isoform H is a critical factor for seed germination under anoxia. The expression of the rice α-amylase RAmylA gene was repressed when the seeds germinated under hypoxia or anoxia. The interactions between oxygen stress, gibberel-lin, and carbon metabolites on the expression of α-amylase in rice are discussed. |
| first_indexed | 2024-12-11T05:29:24Z |
| format | Article |
| id | doaj.art-2bf0401b450e474fb7cd0c546b081d9b |
| institution | Directory Open Access Journal |
| issn | 1343-943X 1349-1008 |
| language | English |
| last_indexed | 2024-12-11T05:29:24Z |
| publishDate | 2000-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Production Science |
| spelling | doaj.art-2bf0401b450e474fb7cd0c546b081d9b2022-12-22T01:19:29ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10082000-01-0131323710.1626/pps.3.3211644390Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged SoilJirong Huang0Kyoko Toyofuku1Junji Yamaguchi2Shigemi Akita3The University of TokyoNagoya University, Chikusa-kuNagoya University, Chikusa-kuThe University of TokyoA significant difference in the seed germinability was observed between the two rice cultivars, ‘Nipponbare’ and ‘Suweon 287’, under anoxia (i.e., during germination in submerged soil at 18°C), although little difference was seen under aerobic (in air) or hypoxic (in water) conditions. The number of α-amylase isoforms synthesized in germinating seeds was inversely proportional to the O2 concentrations at the early germination stage. The formation of isoform B was promoted by oxygen supply, while isoform H was undetectable if the seeds were unable to germinate. The activity of isoform H was highly correlated with the coleoptile length in the submerged soil at 18°C, indicating that isoform H is a critical factor for seed germination under anoxia. The expression of the rice α-amylase RAmylA gene was repressed when the seeds germinated under hypoxia or anoxia. The interactions between oxygen stress, gibberel-lin, and carbon metabolites on the expression of α-amylase in rice are discussed.http://dx.doi.org/10.1626/pps.3.32α-AmylaseColeoptile elongationGene expressionGerminationIsoformRiceSubmerged soil. |
| spellingShingle | Jirong Huang Kyoko Toyofuku Junji Yamaguchi Shigemi Akita Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil Plant Production Science α-Amylase Coleoptile elongation Gene expression Germination Isoform Rice Submerged soil. |
| title | Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil |
| title_full | Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil |
| title_fullStr | Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil |
| title_full_unstemmed | Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil |
| title_short | Expression of α-Amylase Isoforms and the RAmylA Gene in Rice (Oryza sativa L.) during Seed Germination, and its Relationship with Coleoptile Length in Submerged Soil |
| title_sort | expression of α amylase isoforms and the ramyla gene in rice oryza sativa l during seed germination and its relationship with coleoptile length in submerged soil |
| topic | α-Amylase Coleoptile elongation Gene expression Germination Isoform Rice Submerged soil. |
| url | http://dx.doi.org/10.1626/pps.3.32 |
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