Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes

Osteoarthritis (OA) is the most common cause of joint pain, but there’s still no satisfying treatment that can inhibit degradation of articular cartilage in OA. One approach that is being widely studied is through activation of PPAR-γ. Many studies have shown effect of PPAR-γ on OA either in vitro o...

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Main Authors: Rena Normasari, Anis Murniati
Format: Article
Language:English
Published: University of Jember, Faculty of Medicine 2015-10-01
Series:Journal of Agromedicine and Medical Sciences (AMS)
Online Access:https://jurnal.unej.ac.id/index.php/JAMS/article/view/1954
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author Rena Normasari
Anis Murniati
author_facet Rena Normasari
Anis Murniati
author_sort Rena Normasari
collection DOAJ
description Osteoarthritis (OA) is the most common cause of joint pain, but there’s still no satisfying treatment that can inhibit degradation of articular cartilage in OA. One approach that is being widely studied is through activation of PPAR-γ. Many studies have shown effect of PPAR-γ on OA either in vitro or in vivo in animal model. On the other hand, the main risk factor of OA is obesity. Obesity generally followed by increased of leptin level due to leptin resistance. The aim of this study is to examine the effect of pioglitazone as PPAR-γ agonist in reducing the level of MMP-9 and MMP-13 in OA chondrocyte with hyperleptinemia. MMP-9 and MMP-13 are key role enzymes that degrades cartilage in OA. This study use IL-1β-induced chondrocyte exposed to pioglitazone. Pioglitazone 0,1μM, 1μM, and 10μM reduce MMP-9 and MMP-13 level. Whereas pioglitazone together with leptin 10μg/ml reduce the decrease level of MMPs compared to the group without leptin. Leptin inhibit the effect of pioglitazone in reducing the level of MMPs. Keywords: OA, leptin, pioglitazone, MMP-9, MMP-13
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spelling doaj.art-2bf241e4e8b14f79a4c1d06c8e9bd4142023-02-03T09:29:23ZengUniversity of Jember, Faculty of MedicineJournal of Agromedicine and Medical Sciences (AMS)2460-90482714-56542015-10-011212181954Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced ChondrocytesRena Normasari0Anis Murniati1Laboratorium Patologi Anatomi Fakultas Kedokteran Universitas JemberSTIKES Hutama Abdi Husada TulungagungOsteoarthritis (OA) is the most common cause of joint pain, but there’s still no satisfying treatment that can inhibit degradation of articular cartilage in OA. One approach that is being widely studied is through activation of PPAR-γ. Many studies have shown effect of PPAR-γ on OA either in vitro or in vivo in animal model. On the other hand, the main risk factor of OA is obesity. Obesity generally followed by increased of leptin level due to leptin resistance. The aim of this study is to examine the effect of pioglitazone as PPAR-γ agonist in reducing the level of MMP-9 and MMP-13 in OA chondrocyte with hyperleptinemia. MMP-9 and MMP-13 are key role enzymes that degrades cartilage in OA. This study use IL-1β-induced chondrocyte exposed to pioglitazone. Pioglitazone 0,1μM, 1μM, and 10μM reduce MMP-9 and MMP-13 level. Whereas pioglitazone together with leptin 10μg/ml reduce the decrease level of MMPs compared to the group without leptin. Leptin inhibit the effect of pioglitazone in reducing the level of MMPs. Keywords: OA, leptin, pioglitazone, MMP-9, MMP-13https://jurnal.unej.ac.id/index.php/JAMS/article/view/1954
spellingShingle Rena Normasari
Anis Murniati
Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes
Journal of Agromedicine and Medical Sciences (AMS)
title Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes
title_full Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes
title_fullStr Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes
title_full_unstemmed Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes
title_short Leptin Inhibit the Effect of Pioglitazone in Reducing MMP-9 and MMP-13 of IL-1β-Induced Chondrocytes
title_sort leptin inhibit the effect of pioglitazone in reducing mmp 9 and mmp 13 of il 1β induced chondrocytes
url https://jurnal.unej.ac.id/index.php/JAMS/article/view/1954
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AT anismurniati leptininhibittheeffectofpioglitazoneinreducingmmp9andmmp13ofil1binducedchondrocytes