In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria
Background and Aim: Mutans streptococci are the main cause of tooth decay. Application of natural materials as mouthwash has been effective in reducing the bacterial count. This study aimed to assess the antimicrobial effects of rose water and pomegranate vinegar in comparison with Persica mouthwash...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Islamic Dental Association of Iran
2015-07-01
|
| Series: | Journal of Islamic Dental Association of Iran |
| Subjects: | |
| Online Access: | http://jidai.ir/browse.php?a_code=A-10-1-834&slc_lang=en&sid=1 |
| _version_ | 1818190017701347328 |
|---|---|
| author | Fatemeh Ramezanalizadeh Mohammad Rabbani Maryam Khoroushi Azam Aliasghari |
| author_facet | Fatemeh Ramezanalizadeh Mohammad Rabbani Maryam Khoroushi Azam Aliasghari |
| author_sort | Fatemeh Ramezanalizadeh |
| collection | DOAJ |
| description | Background and Aim: Mutans streptococci are the main cause of tooth decay. Application of natural materials as mouthwash has been effective in reducing the bacterial count. This study aimed to assess the antimicrobial effects of rose water and pomegranate vinegar in comparison with Persica mouthwash on two oral bacteria responsible for tooth decay.
Materials and Methods: Strongly adherent strains of Streptococcus mutans and Streptococcus sobrinus were selected for this in vitro study. Antimicrobial effects of pomegranate vinegar and rose water on microbial count in the biofilm and adhesion potential of bacteria were evaluated by microtiter plate method. Also, the well-plate technique was used to assess the effect of rose water and pomegranate vinegar in comparison with Persica mouthwash on bacterial growth and proliferation. The obtained resulted were analyzed by one-way ANOVA and Tukey’s post hoc test using Graph Pad Prism version 5 software. Level of significance was set at P=0.05 with 95% confidence interval.
Results: Pomegranate vinegar, rose water and Persica decreased plaque formation by S. mutans by 93%, 80% and 68%, respectively. These values for the S. sobrinus were 92%, 57% and 48%, respectively (all Ps<0.001). Pomegranate vinegar was more effective than the other two materials (P<0.001). However, none of these materials eliminated the biofilm. Pomegranate vinegar and Persica mouthwash inhibited the growth of the afore-mentioned bacteria.
Conclusion: Within the limitations of this study, it seems that pomegranate vinegar and rose water have the potential to prevent or control the proliferation of S. mutans and S. sobrinus. |
| first_indexed | 2024-12-11T23:52:02Z |
| format | Article |
| id | doaj.art-2bf35c8249714d17b7e7bbd134b1bed0 |
| institution | Directory Open Access Journal |
| issn | 2383-3041 2383-3041 |
| language | English |
| last_indexed | 2024-12-11T23:52:02Z |
| publishDate | 2015-07-01 |
| publisher | Islamic Dental Association of Iran |
| record_format | Article |
| series | Journal of Islamic Dental Association of Iran |
| spelling | doaj.art-2bf35c8249714d17b7e7bbd134b1bed02022-12-22T00:45:28ZengIslamic Dental Association of IranJournal of Islamic Dental Association of Iran2383-30412383-30412015-07-01273150157In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral BacteriaFatemeh Ramezanalizadeh0Mohammad Rabbani1Maryam Khoroushi2Azam Aliasghari3 Graduate Student, Department of Microbiology, School of Biology, Isfahan University of Medical Sciences, Isfahan, Iran Associate Professor, Department of Microbiology, School of Biology, Isfahan University of Medical Sciences, Isfahan, Iran Professor, Department of Operative Dentistry, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran Postgraduate Student, Department of Microbiology, School of Biology, Isfahan University of Medical Sciences, Isfahan, Iran Background and Aim: Mutans streptococci are the main cause of tooth decay. Application of natural materials as mouthwash has been effective in reducing the bacterial count. This study aimed to assess the antimicrobial effects of rose water and pomegranate vinegar in comparison with Persica mouthwash on two oral bacteria responsible for tooth decay. Materials and Methods: Strongly adherent strains of Streptococcus mutans and Streptococcus sobrinus were selected for this in vitro study. Antimicrobial effects of pomegranate vinegar and rose water on microbial count in the biofilm and adhesion potential of bacteria were evaluated by microtiter plate method. Also, the well-plate technique was used to assess the effect of rose water and pomegranate vinegar in comparison with Persica mouthwash on bacterial growth and proliferation. The obtained resulted were analyzed by one-way ANOVA and Tukey’s post hoc test using Graph Pad Prism version 5 software. Level of significance was set at P=0.05 with 95% confidence interval. Results: Pomegranate vinegar, rose water and Persica decreased plaque formation by S. mutans by 93%, 80% and 68%, respectively. These values for the S. sobrinus were 92%, 57% and 48%, respectively (all Ps<0.001). Pomegranate vinegar was more effective than the other two materials (P<0.001). However, none of these materials eliminated the biofilm. Pomegranate vinegar and Persica mouthwash inhibited the growth of the afore-mentioned bacteria. Conclusion: Within the limitations of this study, it seems that pomegranate vinegar and rose water have the potential to prevent or control the proliferation of S. mutans and S. sobrinus.http://jidai.ir/browse.php?a_code=A-10-1-834&slc_lang=en&sid=1Dental CariesMouthwashesStreptococcus mutansStreptococcus sobrinus |
| spellingShingle | Fatemeh Ramezanalizadeh Mohammad Rabbani Maryam Khoroushi Azam Aliasghari In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria Journal of Islamic Dental Association of Iran Dental Caries Mouthwashes Streptococcus mutans Streptococcus sobrinus |
| title | In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria |
| title_full | In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria |
| title_fullStr | In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria |
| title_full_unstemmed | In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria |
| title_short | In Vitro Assessment of Antibacterial Activity of Pomegranate Vinegar and Rose Water Compared with Persica Mouthwash against Oral Bacteria |
| title_sort | in vitro assessment of antibacterial activity of pomegranate vinegar and rose water compared with persica mouthwash against oral bacteria |
| topic | Dental Caries Mouthwashes Streptococcus mutans Streptococcus sobrinus |
| url | http://jidai.ir/browse.php?a_code=A-10-1-834&slc_lang=en&sid=1 |
| work_keys_str_mv | AT fatemehramezanalizadeh invitroassessmentofantibacterialactivityofpomegranatevinegarandrosewatercomparedwithpersicamouthwashagainstoralbacteria AT mohammadrabbani invitroassessmentofantibacterialactivityofpomegranatevinegarandrosewatercomparedwithpersicamouthwashagainstoralbacteria AT maryamkhoroushi invitroassessmentofantibacterialactivityofpomegranatevinegarandrosewatercomparedwithpersicamouthwashagainstoralbacteria AT azamaliasghari invitroassessmentofantibacterialactivityofpomegranatevinegarandrosewatercomparedwithpersicamouthwashagainstoralbacteria |