Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications
Abstract Background and aim Galectins have been recently tackled by many researchers in the field of cancer due to their role in tumorigenesis, disease progression, and metastasis. Thus, they are currently involved in biomarkers research on several types of cancer. In ovarian cancers, few studies we...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-022-02738-4 |
| _version_ | 1798032477451714560 |
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| author | Radwa Mansour Mohamed Athar Emam Mahmoud M. Abdelfattah Abdel-Mageed Ismail Abdel-Mageed Mohamed A. Abdelhafeez Reham Helwa |
| author_facet | Radwa Mansour Mohamed Athar Emam Mahmoud M. Abdelfattah Abdel-Mageed Ismail Abdel-Mageed Mohamed A. Abdelhafeez Reham Helwa |
| author_sort | Radwa Mansour Mohamed |
| collection | DOAJ |
| description | Abstract Background and aim Galectins have been recently tackled by many researchers in the field of cancer due to their role in tumorigenesis, disease progression, and metastasis. Thus, they are currently involved in biomarkers research on several types of cancer. In ovarian cancers, few studies were carried out to evaluate galectins expression profiling. Hence, our present study was executed to evaluate the mRNA expression of galectins -1, -3, -4, -8, and -9 in epithelial ovarian cancers. Methods Fifty-six tumor samples of ovarian carcinomas were analyzed for mRNA expression using qRT-PCR, and fold-changes were calculated in comparison to tissue samples of 26 women with normal ovaries. Results The results of the present paper emphasize the importance of galectins as predictors for targeted therapy. LGALS1, LGALS3, LGALS4, LGALS8, and LGALS9 were found to be mostly overexpressed in ovarian carcinoma patients with the following percentage: 78.6%, 92.9%, 66.1%, 87.5%, and 85.7% respectively. Moreover, galectins -3 and -9 were found to be significantly elevated with lymph node metastasis (p = 0.044 and p = 0.011). Also, upregulation of galectin-1 and -9 were statistically significant in stages IIB, IIC, and IIIB (p = 0.002) in FIGO staging. CA19.9 is positively correlated to galectin-4 expression (p = 0.039). Conclusion Our findings strengthen the role of galectins in carcinogenesis, disease progression, and lymphnode metastasis in ovarian carcinomas. And since these galectins are mostly overexpressed, they could be promising markers for targeted therapy to reduce disease progression and metastasis process. |
| first_indexed | 2024-04-11T20:14:31Z |
| format | Article |
| id | doaj.art-2bf9f25569da47b3b5f1b299e844a179 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-11T20:14:31Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-2bf9f25569da47b3b5f1b299e844a1792022-12-22T04:05:01ZengBMCWorld Journal of Surgical Oncology1477-78192022-09-012011910.1186/s12957-022-02738-4Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implicationsRadwa Mansour Mohamed0Athar Emam1Mahmoud M. Abdelfattah2Abdel-Mageed Ismail Abdel-Mageed3Mohamed A. Abdelhafeez4Reham Helwa5Department of Obstetrics and Gynaecology, Faculty of Medicine, Ain Shams UniversityDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Ain Shams UniversityMolecular Cancer Biology group, Zoology Department, Faculty of Science, Ain Shams UniversityDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Ain Shams UniversityDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Ain Shams UniversityMolecular Cancer Biology group, Zoology Department, Faculty of Science, Ain Shams UniversityAbstract Background and aim Galectins have been recently tackled by many researchers in the field of cancer due to their role in tumorigenesis, disease progression, and metastasis. Thus, they are currently involved in biomarkers research on several types of cancer. In ovarian cancers, few studies were carried out to evaluate galectins expression profiling. Hence, our present study was executed to evaluate the mRNA expression of galectins -1, -3, -4, -8, and -9 in epithelial ovarian cancers. Methods Fifty-six tumor samples of ovarian carcinomas were analyzed for mRNA expression using qRT-PCR, and fold-changes were calculated in comparison to tissue samples of 26 women with normal ovaries. Results The results of the present paper emphasize the importance of galectins as predictors for targeted therapy. LGALS1, LGALS3, LGALS4, LGALS8, and LGALS9 were found to be mostly overexpressed in ovarian carcinoma patients with the following percentage: 78.6%, 92.9%, 66.1%, 87.5%, and 85.7% respectively. Moreover, galectins -3 and -9 were found to be significantly elevated with lymph node metastasis (p = 0.044 and p = 0.011). Also, upregulation of galectin-1 and -9 were statistically significant in stages IIB, IIC, and IIIB (p = 0.002) in FIGO staging. CA19.9 is positively correlated to galectin-4 expression (p = 0.039). Conclusion Our findings strengthen the role of galectins in carcinogenesis, disease progression, and lymphnode metastasis in ovarian carcinomas. And since these galectins are mostly overexpressed, they could be promising markers for targeted therapy to reduce disease progression and metastasis process.https://doi.org/10.1186/s12957-022-02738-4Ovarian carcinomaGalectinsmRNAqRT-PCRBiomarkersLymph node metastasis |
| spellingShingle | Radwa Mansour Mohamed Athar Emam Mahmoud M. Abdelfattah Abdel-Mageed Ismail Abdel-Mageed Mohamed A. Abdelhafeez Reham Helwa Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications World Journal of Surgical Oncology Ovarian carcinoma Galectins mRNA qRT-PCR Biomarkers Lymph node metastasis |
| title | Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications |
| title_full | Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications |
| title_fullStr | Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications |
| title_full_unstemmed | Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications |
| title_short | Assessment of galectins -1, -3, -4, -8, and -9 expression in ovarian carcinoma patients with clinical implications |
| title_sort | assessment of galectins 1 3 4 8 and 9 expression in ovarian carcinoma patients with clinical implications |
| topic | Ovarian carcinoma Galectins mRNA qRT-PCR Biomarkers Lymph node metastasis |
| url | https://doi.org/10.1186/s12957-022-02738-4 |
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