Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers
BackgroundExtracellular vesicles (EVs) are submicron membrane-bound vesicles released from various cells, which are emerging as a potential novel biomarker in cardiovascular diseases (CVDs) due to their procoagulatory and prothrombotic properties. However, there is little information about the relat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.907457/full |
| _version_ | 1818551305080143872 |
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| author | Ruihan Zhou Esra Bozbas Keith Allen-Redpath Parveen Yaqoob |
| author_facet | Ruihan Zhou Esra Bozbas Keith Allen-Redpath Parveen Yaqoob |
| author_sort | Ruihan Zhou |
| collection | DOAJ |
| description | BackgroundExtracellular vesicles (EVs) are submicron membrane-bound vesicles released from various cells, which are emerging as a potential novel biomarker in cardiovascular diseases (CVDs) due to their procoagulatory and prothrombotic properties. However, there is little information about the relationships between circulating EVs and conventional and thrombogenic risk markers of CVDs.ObjectiveTo investigate the relationships between circulating EVs, conventional cardiovascular risk markers and thrombogenic markers in subjects with moderate risk of CVDs.DesignSubjects (n = 40) aged 40-70 years with moderate risk of CVDs were recruited and assessed for body mass index, blood pressure and plasma lipid profile, as well as platelet aggregation, clot formation, thrombin generation and fibrinolysis. Numbers of circulating EVs were assessed by Nanoparticle Tracking Analysis and flow cytometry. A range of assays were used to assess the procoagulatory activity of plasma and circulating EVs.ResultsCirculating EV numbers were positively associated with body mass index, blood pressure, plasma triacylglycerol concentration and overall CVD risk. Higher circulating EV numbers were also associated with increased thrombin generation and enhanced clot formation, and EVs isolated from subjects with moderate CVD risk promoted thrombin generation ex vivo. Higher numbers of endothelial-derived EVs were associated with a greater tendency for clot lysis. Plasma triacylglycerol concentration and diastolic blood pressure independently predicted circulating EV numbers, and EV numbers independently predicted aspects of thrombin generation and clot formation and 10-year CVD risk.ConclusionCirculating EVs were strongly associated with both conventional and thrombogenic risk markers of CVDs, and also with overall CVD risk, highlighting a potentially important role for EVs in CVDs. |
| first_indexed | 2024-12-12T08:58:07Z |
| format | Article |
| id | doaj.art-2bfa52b02e7e4e9493ae6a69626d5e76 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-12-12T08:58:07Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-2bfa52b02e7e4e9493ae6a69626d5e762022-12-22T00:29:54ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-05-01910.3389/fcvm.2022.907457907457Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk MarkersRuihan ZhouEsra BozbasKeith Allen-RedpathParveen YaqoobBackgroundExtracellular vesicles (EVs) are submicron membrane-bound vesicles released from various cells, which are emerging as a potential novel biomarker in cardiovascular diseases (CVDs) due to their procoagulatory and prothrombotic properties. However, there is little information about the relationships between circulating EVs and conventional and thrombogenic risk markers of CVDs.ObjectiveTo investigate the relationships between circulating EVs, conventional cardiovascular risk markers and thrombogenic markers in subjects with moderate risk of CVDs.DesignSubjects (n = 40) aged 40-70 years with moderate risk of CVDs were recruited and assessed for body mass index, blood pressure and plasma lipid profile, as well as platelet aggregation, clot formation, thrombin generation and fibrinolysis. Numbers of circulating EVs were assessed by Nanoparticle Tracking Analysis and flow cytometry. A range of assays were used to assess the procoagulatory activity of plasma and circulating EVs.ResultsCirculating EV numbers were positively associated with body mass index, blood pressure, plasma triacylglycerol concentration and overall CVD risk. Higher circulating EV numbers were also associated with increased thrombin generation and enhanced clot formation, and EVs isolated from subjects with moderate CVD risk promoted thrombin generation ex vivo. Higher numbers of endothelial-derived EVs were associated with a greater tendency for clot lysis. Plasma triacylglycerol concentration and diastolic blood pressure independently predicted circulating EV numbers, and EV numbers independently predicted aspects of thrombin generation and clot formation and 10-year CVD risk.ConclusionCirculating EVs were strongly associated with both conventional and thrombogenic risk markers of CVDs, and also with overall CVD risk, highlighting a potentially important role for EVs in CVDs.https://www.frontiersin.org/articles/10.3389/fcvm.2022.907457/fullblood lipidsblood pressurecardiovascular risk markersextracellular vesiclesthrombin |
| spellingShingle | Ruihan Zhou Esra Bozbas Keith Allen-Redpath Parveen Yaqoob Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers Frontiers in Cardiovascular Medicine blood lipids blood pressure cardiovascular risk markers extracellular vesicles thrombin |
| title | Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers |
| title_full | Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers |
| title_fullStr | Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers |
| title_full_unstemmed | Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers |
| title_short | Circulating Extracellular Vesicles Are Strongly Associated With Cardiovascular Risk Markers |
| title_sort | circulating extracellular vesicles are strongly associated with cardiovascular risk markers |
| topic | blood lipids blood pressure cardiovascular risk markers extracellular vesicles thrombin |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.907457/full |
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