Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense
Abstract Background Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic c...
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| Format: | Article |
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BMC
2021-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-08144-6 |
| _version_ | 1831713304101257216 |
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| author | Yilian He Hua Chen Jun Zhao Yuxia Yang Bin Yang Liang Feng Yiguan Zhang Ping Wei Dabin Hou Junning Zhao Ma Yu |
| author_facet | Yilian He Hua Chen Jun Zhao Yuxia Yang Bin Yang Liang Feng Yiguan Zhang Ping Wei Dabin Hou Junning Zhao Ma Yu |
| author_sort | Yilian He |
| collection | DOAJ |
| description | Abstract Background Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic composition and the expressed genes involved in saikosaponin biosynthetic pathways among four organs (the root, flower, stem, and leaf) of B. chinense to discover the genes related to the saikosaponin biosynthetic pathway. Results Transcript and metabolite profiles were generated through high-throughput RNA-sequencing (RNA-seq) data analysis and liquid chromatography tandem mass spectrometry, respectively. Evaluation of saikosaponin contents and transcriptional changes showed 152 strong correlations (P < 0.05) over 3 compounds and 77 unigenes. These unigenes belonged to eight gene families: the acetoacetyl CoA transferase (AACT) (6), HMG-CoA synthase (HMGS) (2), HMG-CoA reductase (HMGR) (2), mevalonate diphosphate decarboxylase (MVD) (1), 1-deoxy-D-xylulose-5-phosphate synthase (DXS) (3), farnesyl diphosphate synthase (FPPS) (11), β-amyrin synthase (β-AS) (13) and cytochrome P450 enzymes (P450s) (39) families. Conclusions Our results investigated the diversity of the saikosaponin triterpene biosynthetic pathway in the roots, stems, leaves and flowers of B. chinese by integrated transcriptomic and metabolomic analysis, implying that manipulation of P450s genes such as Bc95697 and Bc35434 might improve saikosaponin biosynthesis. This is a good candidate for the genetic improvement of this important medicinal plant. |
| first_indexed | 2024-12-20T23:38:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-20T23:38:35Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-2bfa5a3cca514790b2e7059ddca266562022-12-21T19:23:08ZengBMCBMC Genomics1471-21642021-11-0122111410.1186/s12864-021-08144-6Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinenseYilian He0Hua Chen1Jun Zhao2Yuxia Yang3Bin Yang4Liang Feng5Yiguan Zhang6Ping Wei7Dabin Hou8Junning Zhao9Ma Yu10School of life science and engineering, Southwest University of Science and TechnologySchool of life science and engineering, Southwest University of Science and TechnologySchool of life science and engineering, Southwest University of Science and TechnologyInstitute of Medicinal Plant Resources, Sichuan Academy of Traditional Chinese Medicine SciencesSchool of life science and engineering, Southwest University of Science and TechnologySchool of life science and engineering, Southwest University of Science and TechnologySichuan Institute for Translational Chinese MedicineSichuan Institute for Translational Chinese MedicineSchool of life science and engineering, Southwest University of Science and TechnologySichuan Institute for Translational Chinese MedicineSchool of life science and engineering, Southwest University of Science and TechnologyAbstract Background Bupleurum chinense DC. is a widely used traditional Chinese medicinal plant. Saikosaponins are the major bioactive constituents of B. chinense, but relatively little is known about saikosaponin biosynthesis. In the present study, we performed an integrated analysis of metabolic composition and the expressed genes involved in saikosaponin biosynthetic pathways among four organs (the root, flower, stem, and leaf) of B. chinense to discover the genes related to the saikosaponin biosynthetic pathway. Results Transcript and metabolite profiles were generated through high-throughput RNA-sequencing (RNA-seq) data analysis and liquid chromatography tandem mass spectrometry, respectively. Evaluation of saikosaponin contents and transcriptional changes showed 152 strong correlations (P < 0.05) over 3 compounds and 77 unigenes. These unigenes belonged to eight gene families: the acetoacetyl CoA transferase (AACT) (6), HMG-CoA synthase (HMGS) (2), HMG-CoA reductase (HMGR) (2), mevalonate diphosphate decarboxylase (MVD) (1), 1-deoxy-D-xylulose-5-phosphate synthase (DXS) (3), farnesyl diphosphate synthase (FPPS) (11), β-amyrin synthase (β-AS) (13) and cytochrome P450 enzymes (P450s) (39) families. Conclusions Our results investigated the diversity of the saikosaponin triterpene biosynthetic pathway in the roots, stems, leaves and flowers of B. chinese by integrated transcriptomic and metabolomic analysis, implying that manipulation of P450s genes such as Bc95697 and Bc35434 might improve saikosaponin biosynthesis. This is a good candidate for the genetic improvement of this important medicinal plant.https://doi.org/10.1186/s12864-021-08144-6Bupleurum chinense DC. TranscriptomeMetabolomeSaikosaponin biosynthetic pathway |
| spellingShingle | Yilian He Hua Chen Jun Zhao Yuxia Yang Bin Yang Liang Feng Yiguan Zhang Ping Wei Dabin Hou Junning Zhao Ma Yu Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense BMC Genomics Bupleurum chinense DC. Transcriptome Metabolome Saikosaponin biosynthetic pathway |
| title | Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense |
| title_full | Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense |
| title_fullStr | Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense |
| title_full_unstemmed | Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense |
| title_short | Transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in Bupleurum chinense |
| title_sort | transcriptome and metabolome analysis to reveal major genes of saikosaponin biosynthesis in bupleurum chinense |
| topic | Bupleurum chinense DC. Transcriptome Metabolome Saikosaponin biosynthetic pathway |
| url | https://doi.org/10.1186/s12864-021-08144-6 |
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