Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308
The protein MucR from <i>Brucella abortus</i> has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their...
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/788 |
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| author | Giorgia Borriello Veronica Russo Rubina Paradiso Marita Georgia Riccardi Daniela Criscuolo Gaetano Verde Rosangela Marasco Paolo Vincenzo Pedone Giorgio Galiero Ilaria Baglivo |
| author_facet | Giorgia Borriello Veronica Russo Rubina Paradiso Marita Georgia Riccardi Daniela Criscuolo Gaetano Verde Rosangela Marasco Paolo Vincenzo Pedone Giorgio Galiero Ilaria Baglivo |
| author_sort | Giorgia Borriello |
| collection | DOAJ |
| description | The protein MucR from <i>Brucella abortus</i> has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their eukaryotic hosts. Despite clear evidence of the role of MucR in repressing virulence genes, no study has been carried out so far demonstrating the direct interaction of this protein with the promoter of its target gene <i>babR</i> encoding a LuxR-like regulator repressing <i>virB</i> genes. In this study, we show for the first time the ability of MucR to bind the promoter of <i>babR</i> in electrophoretic mobility shift assays demonstrating a direct role of MucR in repressing this gene. Furthermore, we demonstrate that MucR can bind the <i>virB</i> gene promoter. Analyses by RT-qPCR showed no significant differences in the expression level of <i>virB</i> genes in <i>Brucella abortus</i> CC092 lacking MucR compared to the wild-type <i>Brucella abortus</i> strain, indicating that MucR binding to the <i>virB</i> promoter has little impact on <i>virB</i> gene expression in <i>B. abortus</i> 2308. The MucR modality to bind the two promoters analyzed supports our previous hypothesis that this is a histone-like protein never found before in <i>Brucella</i>. |
| first_indexed | 2024-03-10T19:43:34Z |
| format | Article |
| id | doaj.art-2bfaa4375e254bf1b2dbfe1b79aca24c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:43:34Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-2bfaa4375e254bf1b2dbfe1b79aca24c2023-11-20T01:00:33ZengMDPI AGBiomolecules2218-273X2020-05-0110578810.3390/biom10050788Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308Giorgia Borriello0Veronica Russo1Rubina Paradiso2Marita Georgia Riccardi3Daniela Criscuolo4Gaetano Verde5Rosangela Marasco6Paolo Vincenzo Pedone7Giorgio Galiero8Ilaria Baglivo9Experimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, via Vivaldi—43, 81100 Caserta, ItalyExperimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, ItalyExperimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, ItalyExperimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, ItalyInstitute of Genetics and Biophysics (IGB) “Adriano Buzzati-Traverso”, Consiglio Nazionale delle Ricerche (CNR), 80134 Naples, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, via Vivaldi—43, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, via Vivaldi—43, 81100 Caserta, ItalyExperimental Zooprophylactic Institute of Southern Italy, via Salute, 2, 80055 Portici, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, via Vivaldi—43, 81100 Caserta, ItalyThe protein MucR from <i>Brucella abortus</i> has been described as a transcriptional regulator of many virulence genes. It is a member of the Ros/MucR family comprising proteins that control the expression of genes important for the successful interaction of α-proteobacteria with their eukaryotic hosts. Despite clear evidence of the role of MucR in repressing virulence genes, no study has been carried out so far demonstrating the direct interaction of this protein with the promoter of its target gene <i>babR</i> encoding a LuxR-like regulator repressing <i>virB</i> genes. In this study, we show for the first time the ability of MucR to bind the promoter of <i>babR</i> in electrophoretic mobility shift assays demonstrating a direct role of MucR in repressing this gene. Furthermore, we demonstrate that MucR can bind the <i>virB</i> gene promoter. Analyses by RT-qPCR showed no significant differences in the expression level of <i>virB</i> genes in <i>Brucella abortus</i> CC092 lacking MucR compared to the wild-type <i>Brucella abortus</i> strain, indicating that MucR binding to the <i>virB</i> promoter has little impact on <i>virB</i> gene expression in <i>B. abortus</i> 2308. The MucR modality to bind the two promoters analyzed supports our previous hypothesis that this is a histone-like protein never found before in <i>Brucella</i>.https://www.mdpi.com/2218-273X/10/5/788<i>Brucella abortus</i><i>virB</i> genesMucRBabRH-NSvirulence gene expression regulation |
| spellingShingle | Giorgia Borriello Veronica Russo Rubina Paradiso Marita Georgia Riccardi Daniela Criscuolo Gaetano Verde Rosangela Marasco Paolo Vincenzo Pedone Giorgio Galiero Ilaria Baglivo Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308 Biomolecules <i>Brucella abortus</i> <i>virB</i> genes MucR BabR H-NS virulence gene expression regulation |
| title | Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308 |
| title_full | Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308 |
| title_fullStr | Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308 |
| title_full_unstemmed | Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308 |
| title_short | Different Impacts of MucR Binding to the <i>babR</i> and <i>virB</i> Promoters on Gene Expression in <i>Brucella abortus</i> 2308 |
| title_sort | different impacts of mucr binding to the i babr i and i virb i promoters on gene expression in i brucella abortus i 2308 |
| topic | <i>Brucella abortus</i> <i>virB</i> genes MucR BabR H-NS virulence gene expression regulation |
| url | https://www.mdpi.com/2218-273X/10/5/788 |
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