Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence
Plasmid and chromosomal genes encode determinants of virulence for Yersinia pestis, the causative agent of plague. However, in vitro, Y. pestis genome is very plastic and several changes have been described. To evaluate the alterations in the plasmid content of the cultures in vitro and the impact o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2004-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700011 |
| _version_ | 1797756760247762944 |
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| author | TC Leal-Balbino NC Leal CV Lopes AMP de Almeida |
| author_facet | TC Leal-Balbino NC Leal CV Lopes AMP de Almeida |
| author_sort | TC Leal-Balbino |
| collection | DOAJ |
| description | Plasmid and chromosomal genes encode determinants of virulence for Yersinia pestis, the causative agent of plague. However, in vitro, Y. pestis genome is very plastic and several changes have been described. To evaluate the alterations in the plasmid content of the cultures in vitro and the impact of the alterations to their pathogenicity, three Y. pestis isolates were submitted to serial subculture, analysis of the plasmid content, and testing for the presence of characteristic genes in each plasmid of colonies selected after subculture. Different results were obtained with each strain. The plasmid content of one of them was shown to be stable; no apparent alteration was produced through 32 subcultures. In the other two strains, several alterations were observed. LD50 in mice of the parental strains and the derived cultures with different plasmid content were compared. No changes in the virulence plasmid content could be specifically correlated with changes in the LD50. |
| first_indexed | 2024-03-12T18:06:12Z |
| format | Article |
| id | doaj.art-2bfb94c23ddc4dd09e7bed85fdceeecd |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:06:12Z |
| publishDate | 2004-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-2bfb94c23ddc4dd09e7bed85fdceeecd2023-08-02T09:28:26ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602004-11-0199772773210.1590/S0074-02762004000700011Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulenceTC Leal-BalbinoNC LealCV LopesAMP de AlmeidaPlasmid and chromosomal genes encode determinants of virulence for Yersinia pestis, the causative agent of plague. However, in vitro, Y. pestis genome is very plastic and several changes have been described. To evaluate the alterations in the plasmid content of the cultures in vitro and the impact of the alterations to their pathogenicity, three Y. pestis isolates were submitted to serial subculture, analysis of the plasmid content, and testing for the presence of characteristic genes in each plasmid of colonies selected after subculture. Different results were obtained with each strain. The plasmid content of one of them was shown to be stable; no apparent alteration was produced through 32 subcultures. In the other two strains, several alterations were observed. LD50 in mice of the parental strains and the derived cultures with different plasmid content were compared. No changes in the virulence plasmid content could be specifically correlated with changes in the LD50.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700011plagueYersinia pestis plasmidsvirulence |
| spellingShingle | TC Leal-Balbino NC Leal CV Lopes AMP de Almeida Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence Memorias do Instituto Oswaldo Cruz plague Yersinia pestis plasmids virulence |
| title | Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence |
| title_full | Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence |
| title_fullStr | Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence |
| title_full_unstemmed | Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence |
| title_short | Differences in the stability of the plasmids of Yersinia pestis cultures in vitro: impact on virulence |
| title_sort | differences in the stability of the plasmids of yersinia pestis cultures in vitro impact on virulence |
| topic | plague Yersinia pestis plasmids virulence |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700011 |
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