Single-cell-precision microplasma-induced cancer cell apoptosis.
The issue of single-cell control has recently attracted enormous interest. However, in spite of the presently achievable intracellular-level physiological probing through bio-photonics, nano-probe-based, and some other techniques, the issue of inducing selective, single-cell-precision apoptosis, wit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4074162?pdf=render |
| _version_ | 1811206360569741312 |
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| author | Xiao Tan Shasha Zhao Qian Lei Xinpei Lu Guangyuan He Kostya Ostrikov |
| author_facet | Xiao Tan Shasha Zhao Qian Lei Xinpei Lu Guangyuan He Kostya Ostrikov |
| author_sort | Xiao Tan |
| collection | DOAJ |
| description | The issue of single-cell control has recently attracted enormous interest. However, in spite of the presently achievable intracellular-level physiological probing through bio-photonics, nano-probe-based, and some other techniques, the issue of inducing selective, single-cell-precision apoptosis, without affecting neighbouring cells remains essentially open. Here we resolve this issue and report on the effective single-cell-precision cancer cell treatment using the reactive chemistry of the localized corona-type plasma discharge around a needle-like electrode with the spot size ∼1 µm. When the electrode is positioned with the micrometer precision against a selected cell, a focused and highly-localized micro-plasma discharge induces apoptosis in the selected individual HepG2 and HeLa cancer cells only, without affecting any surrounding cells, even in small cell clusters. This is confirmed by the real-time monitoring of the morphological and structural changes at the cellular and cell nucleus levels after the plasma exposure. |
| first_indexed | 2024-04-12T03:47:14Z |
| format | Article |
| id | doaj.art-2bfc60a007524828974f48f07084c590 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:47:14Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2bfc60a007524828974f48f07084c5902022-12-22T03:49:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10129910.1371/journal.pone.0101299Single-cell-precision microplasma-induced cancer cell apoptosis.Xiao TanShasha ZhaoQian LeiXinpei LuGuangyuan HeKostya OstrikovThe issue of single-cell control has recently attracted enormous interest. However, in spite of the presently achievable intracellular-level physiological probing through bio-photonics, nano-probe-based, and some other techniques, the issue of inducing selective, single-cell-precision apoptosis, without affecting neighbouring cells remains essentially open. Here we resolve this issue and report on the effective single-cell-precision cancer cell treatment using the reactive chemistry of the localized corona-type plasma discharge around a needle-like electrode with the spot size ∼1 µm. When the electrode is positioned with the micrometer precision against a selected cell, a focused and highly-localized micro-plasma discharge induces apoptosis in the selected individual HepG2 and HeLa cancer cells only, without affecting any surrounding cells, even in small cell clusters. This is confirmed by the real-time monitoring of the morphological and structural changes at the cellular and cell nucleus levels after the plasma exposure.http://europepmc.org/articles/PMC4074162?pdf=render |
| spellingShingle | Xiao Tan Shasha Zhao Qian Lei Xinpei Lu Guangyuan He Kostya Ostrikov Single-cell-precision microplasma-induced cancer cell apoptosis. PLoS ONE |
| title | Single-cell-precision microplasma-induced cancer cell apoptosis. |
| title_full | Single-cell-precision microplasma-induced cancer cell apoptosis. |
| title_fullStr | Single-cell-precision microplasma-induced cancer cell apoptosis. |
| title_full_unstemmed | Single-cell-precision microplasma-induced cancer cell apoptosis. |
| title_short | Single-cell-precision microplasma-induced cancer cell apoptosis. |
| title_sort | single cell precision microplasma induced cancer cell apoptosis |
| url | http://europepmc.org/articles/PMC4074162?pdf=render |
| work_keys_str_mv | AT xiaotan singlecellprecisionmicroplasmainducedcancercellapoptosis AT shashazhao singlecellprecisionmicroplasmainducedcancercellapoptosis AT qianlei singlecellprecisionmicroplasmainducedcancercellapoptosis AT xinpeilu singlecellprecisionmicroplasmainducedcancercellapoptosis AT guangyuanhe singlecellprecisionmicroplasmainducedcancercellapoptosis AT kostyaostrikov singlecellprecisionmicroplasmainducedcancercellapoptosis |