A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters
Blood purification is performed to control cytokines in critically ill patients. The relationship between the clearance (CL) and the membrane area during adsorption is not clear. We hypothesized that the CL increases with the hydrophobic area when hydrophobic binding contributes to cytokine adsorpti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/8/811 |
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| author | Tomoyuki Nakamura Kazuhiro Moriyama Naohide Kuriyama Yoshitaka Hara Satoshi Komatsu Takahiro Kawaji Yu Kato Takuma Ishihara Ayumi Shintani Osamu Nishida |
| author_facet | Tomoyuki Nakamura Kazuhiro Moriyama Naohide Kuriyama Yoshitaka Hara Satoshi Komatsu Takahiro Kawaji Yu Kato Takuma Ishihara Ayumi Shintani Osamu Nishida |
| author_sort | Tomoyuki Nakamura |
| collection | DOAJ |
| description | Blood purification is performed to control cytokines in critically ill patients. The relationship between the clearance (CL) and the membrane area during adsorption is not clear. We hypothesized that the CL increases with the hydrophobic area when hydrophobic binding contributes to cytokine adsorption. We investigated the relationship between the hemofilter membrane area and the CL of the high mobility group box 1 protein (HMGB-1) and interleukin-6 (IL-6). We performed experimental hemofiltration in vitro using polymethyl methacrylate membranes CH-1.8W (1.8 m<sup>2</sup>) and CH-1.0N (1.0 m<sup>2</sup>), as well as polysulfone membrane NV-18X (1.8 m<sup>2</sup>). After adding 100 mg of HMGB1 or 10 μg of IL-6 into the test solution, experimental hemofiltration was conducted for 360 min in a closed-loop circulation system, and the same amount of HMGB1 and IL-6 was added after 180 min. With CH-1.8W and CH-1.0N, both HMGB-1 and IL-6 showed a rapid concentration decrease of more than 70% at 180 min and 360 min after the re-addition. At 15 min, the CL of HMGB-1 was CH-1.8W: 28.4 and CH-1.0N: 19.8, and that of IL-6 was CH-1.8W: 41.1 and CH-1.0N: 25.4. CH-1.8W and CH-1.0N removed HMGB1 and IL-6 by adsorption and CH-1.8W was superior in CL, which increased with a greater membrane area. |
| first_indexed | 2024-03-09T09:52:37Z |
| format | Article |
| id | doaj.art-2bfc9db9246a42dc9a7f213453761fea |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T09:52:37Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-2bfc9db9246a42dc9a7f213453761fea2023-12-01T23:59:16ZengMDPI AGMembranes2077-03752022-08-0112881110.3390/membranes12080811A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate HemofiltersTomoyuki Nakamura0Kazuhiro Moriyama1Naohide Kuriyama2Yoshitaka Hara3Satoshi Komatsu4Takahiro Kawaji5Yu Kato6Takuma Ishihara7Ayumi Shintani8Osamu Nishida9Department of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanLaboratory for Immune Response and Regulatory Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanDepartment of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanDepartment of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanDepartment of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanDepartment of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanDepartment of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanInnovative and Clinical Research Promotion Center, Gifu University Hospital, Gifu 501-1193, JapanDepartment of Medical Statistics, Graduate School of Medicine, Osaka Metropolitan University, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, JapanDepartment of Anesthesiology and Critical Care Medicine, Fujita Health University School of Medicine, Toyoake 470-1192, JapanBlood purification is performed to control cytokines in critically ill patients. The relationship between the clearance (CL) and the membrane area during adsorption is not clear. We hypothesized that the CL increases with the hydrophobic area when hydrophobic binding contributes to cytokine adsorption. We investigated the relationship between the hemofilter membrane area and the CL of the high mobility group box 1 protein (HMGB-1) and interleukin-6 (IL-6). We performed experimental hemofiltration in vitro using polymethyl methacrylate membranes CH-1.8W (1.8 m<sup>2</sup>) and CH-1.0N (1.0 m<sup>2</sup>), as well as polysulfone membrane NV-18X (1.8 m<sup>2</sup>). After adding 100 mg of HMGB1 or 10 μg of IL-6 into the test solution, experimental hemofiltration was conducted for 360 min in a closed-loop circulation system, and the same amount of HMGB1 and IL-6 was added after 180 min. With CH-1.8W and CH-1.0N, both HMGB-1 and IL-6 showed a rapid concentration decrease of more than 70% at 180 min and 360 min after the re-addition. At 15 min, the CL of HMGB-1 was CH-1.8W: 28.4 and CH-1.0N: 19.8, and that of IL-6 was CH-1.8W: 41.1 and CH-1.0N: 25.4. CH-1.8W and CH-1.0N removed HMGB1 and IL-6 by adsorption and CH-1.8W was superior in CL, which increased with a greater membrane area.https://www.mdpi.com/2077-0375/12/8/811adsorptionblood purificationclearancehemofiltersHMGB-1hydrophobic binding |
| spellingShingle | Tomoyuki Nakamura Kazuhiro Moriyama Naohide Kuriyama Yoshitaka Hara Satoshi Komatsu Takahiro Kawaji Yu Kato Takuma Ishihara Ayumi Shintani Osamu Nishida A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters Membranes adsorption blood purification clearance hemofilters HMGB-1 hydrophobic binding |
| title | A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters |
| title_full | A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters |
| title_fullStr | A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters |
| title_full_unstemmed | A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters |
| title_short | A Larger Membrane Area Increases Cytokine Removal in Polymethyl Methacrylate Hemofilters |
| title_sort | larger membrane area increases cytokine removal in polymethyl methacrylate hemofilters |
| topic | adsorption blood purification clearance hemofilters HMGB-1 hydrophobic binding |
| url | https://www.mdpi.com/2077-0375/12/8/811 |
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