<i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier
Non-alcoholic fatty liver disease (NAFLD) has become a highly concerned health issue in modern society. Due to the attentions of probiotics in the prevention of NAFLD, it is necessary to further clarify their roles. In this study, the methionine and choline-deficient (MCD) diet induced NAFLD rats mo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/12/3126 |
| _version_ | 1797504609956134912 |
|---|---|
| author | Yanping Wang Yang Zhang Jingnan Yang Haoran Li Jinju Wang Weitao Geng |
| author_facet | Yanping Wang Yang Zhang Jingnan Yang Haoran Li Jinju Wang Weitao Geng |
| author_sort | Yanping Wang |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) has become a highly concerned health issue in modern society. Due to the attentions of probiotics in the prevention of NAFLD, it is necessary to further clarify their roles. In this study, the methionine and choline-deficient (MCD) diet induced NAFLD rats model were constructed and treated with strain <i>L</i>. <i>plantarum</i> MA2 by intragastric administration once a day at a dose of 1 × 10<sup>8</sup> cfu/g.bw. After 56 days of the therapeutic intervention, the lipid metabolism and the liver pathological damage of the NAFLD rats were significantly improved. The content of total cholesterol (TC) and total triglyceride (TG) in serum were significantly lower than that in the NAFLD group (<i>p</i> < 0.05). Meanwhile, the intestinal mucosal barrier and the structure of intestinal microbiota were also improved. The villi length and the expression of claudin-1 was significantly higher than that in the NAFLD group (<i>p</i> < 0.05). Then, by detecting the content of LPS in the serum and the LPS-TLR4 pathway in the liver, we can conclude that Lactobacillus plantarum MA2 could reduce the LPS by regulating the gut microecology, thereby inhibit the activation of LPS-TLR4 and it downstream inflammatory signaling pathways. Therefore, our studies on rats showed that <i>L</i><i>. plantarum</i> MA2 has the potential application in the alleviation of NAFLD. Moreover, based on the application of the strain in food industry, this study is of great significance to the development of new therapeutic strategy for NAFLD. |
| first_indexed | 2024-03-10T04:07:00Z |
| format | Article |
| id | doaj.art-2bfcfeec0b8c47a08551135086b0e235 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:07:00Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2bfcfeec0b8c47a08551135086b0e2352023-11-23T08:19:11ZengMDPI AGFoods2304-81582021-12-011012312610.3390/foods10123126<i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal BarrierYanping Wang0Yang Zhang1Jingnan Yang2Haoran Li3Jinju Wang4Weitao Geng5College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, ChinaCollege of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, ChinaNon-alcoholic fatty liver disease (NAFLD) has become a highly concerned health issue in modern society. Due to the attentions of probiotics in the prevention of NAFLD, it is necessary to further clarify their roles. In this study, the methionine and choline-deficient (MCD) diet induced NAFLD rats model were constructed and treated with strain <i>L</i>. <i>plantarum</i> MA2 by intragastric administration once a day at a dose of 1 × 10<sup>8</sup> cfu/g.bw. After 56 days of the therapeutic intervention, the lipid metabolism and the liver pathological damage of the NAFLD rats were significantly improved. The content of total cholesterol (TC) and total triglyceride (TG) in serum were significantly lower than that in the NAFLD group (<i>p</i> < 0.05). Meanwhile, the intestinal mucosal barrier and the structure of intestinal microbiota were also improved. The villi length and the expression of claudin-1 was significantly higher than that in the NAFLD group (<i>p</i> < 0.05). Then, by detecting the content of LPS in the serum and the LPS-TLR4 pathway in the liver, we can conclude that Lactobacillus plantarum MA2 could reduce the LPS by regulating the gut microecology, thereby inhibit the activation of LPS-TLR4 and it downstream inflammatory signaling pathways. Therefore, our studies on rats showed that <i>L</i><i>. plantarum</i> MA2 has the potential application in the alleviation of NAFLD. Moreover, based on the application of the strain in food industry, this study is of great significance to the development of new therapeutic strategy for NAFLD.https://www.mdpi.com/2304-8158/10/12/3126non-alcoholic fatty liver disease<i>L</i><i>. plantarum</i> MA2intestinal microecologymucosal barrier |
| spellingShingle | Yanping Wang Yang Zhang Jingnan Yang Haoran Li Jinju Wang Weitao Geng <i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier Foods non-alcoholic fatty liver disease <i>L</i><i>. plantarum</i> MA2 intestinal microecology mucosal barrier |
| title | <i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier |
| title_full | <i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier |
| title_fullStr | <i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier |
| title_full_unstemmed | <i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier |
| title_short | <i>Lactobacillus plantarum</i> MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier |
| title_sort | i lactobacillus plantarum i ma2 ameliorates methionine and choline deficient diet induced non alcoholic fatty liver disease in rats by improving the intestinal microecology and mucosal barrier |
| topic | non-alcoholic fatty liver disease <i>L</i><i>. plantarum</i> MA2 intestinal microecology mucosal barrier |
| url | https://www.mdpi.com/2304-8158/10/12/3126 |
| work_keys_str_mv | AT yanpingwang ilactobacillusplantarumima2amelioratesmethionineandcholinedeficientdietinducednonalcoholicfattyliverdiseaseinratsbyimprovingtheintestinalmicroecologyandmucosalbarrier AT yangzhang ilactobacillusplantarumima2amelioratesmethionineandcholinedeficientdietinducednonalcoholicfattyliverdiseaseinratsbyimprovingtheintestinalmicroecologyandmucosalbarrier AT jingnanyang ilactobacillusplantarumima2amelioratesmethionineandcholinedeficientdietinducednonalcoholicfattyliverdiseaseinratsbyimprovingtheintestinalmicroecologyandmucosalbarrier AT haoranli ilactobacillusplantarumima2amelioratesmethionineandcholinedeficientdietinducednonalcoholicfattyliverdiseaseinratsbyimprovingtheintestinalmicroecologyandmucosalbarrier AT jinjuwang ilactobacillusplantarumima2amelioratesmethionineandcholinedeficientdietinducednonalcoholicfattyliverdiseaseinratsbyimprovingtheintestinalmicroecologyandmucosalbarrier AT weitaogeng ilactobacillusplantarumima2amelioratesmethionineandcholinedeficientdietinducednonalcoholicfattyliverdiseaseinratsbyimprovingtheintestinalmicroecologyandmucosalbarrier |