Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids
There are now several examples of plant species engineered to synthesise and accumulate nutritionally important polyunsaturated fatty acids in their seed triacylglycerols (TAG). The utilization of such TAG in germinating seeds of such transgenic plants was unknown. In this study, we examined the TAG...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01402/full |
| _version_ | 1818980715730042880 |
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| author | Pushkar Shrestha Damien L Callahan Damien L Callahan Surinder P Singh James Robertson Petrie Xue-Rong Zhou |
| author_facet | Pushkar Shrestha Damien L Callahan Damien L Callahan Surinder P Singh James Robertson Petrie Xue-Rong Zhou |
| author_sort | Pushkar Shrestha |
| collection | DOAJ |
| description | There are now several examples of plant species engineered to synthesise and accumulate nutritionally important polyunsaturated fatty acids in their seed triacylglycerols (TAG). The utilization of such TAG in germinating seeds of such transgenic plants was unknown. In this study, we examined the TAG utilization efficiency during seed germination in transgenic Arabidopsis seeds containing several examples of these fatty acids. Seed TAG species with native fatty acids had higher utilization rate than the TAG species containing transgenically produced polyunsaturated fatty acids. Conversely, quantification of the fatty acid components remaining in the total TAG after early stages of seed germination revealed that the undigested TAGs tended to contain an elevated level of the engineered polyunsaturated fatty acids (PUFA). LC-MS analysis further revealed asymmetrical mobilization rates for the individual TAG species. TAGs which contained multiple PUFA fatty acids were mobilized slower than the species containing single PUFA. The mobilised engineered fatty acids were used in de novo membrane lipid synthesis during seedling development. |
| first_indexed | 2024-12-20T17:19:50Z |
| format | Article |
| id | doaj.art-2c03ea1f0113419e95da0241a48da724 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T17:19:50Z |
| publishDate | 2016-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2c03ea1f0113419e95da0241a48da7242022-12-21T19:31:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-09-01710.3389/fpls.2016.01402222574Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acidsPushkar Shrestha0Damien L Callahan1Damien L Callahan2Surinder P Singh3James Robertson Petrie4Xue-Rong Zhou5CSIRO Agriculture & FoodMetabolomics Australia, School of Botany, University of MelbourneCentre for Chemistry and Biotechnology, School of Life and Environmental Science, Deakin UniversityCSIRO Agriculture & FoodCSIRO Agriculture & FoodCSIRO Agriculture & FoodThere are now several examples of plant species engineered to synthesise and accumulate nutritionally important polyunsaturated fatty acids in their seed triacylglycerols (TAG). The utilization of such TAG in germinating seeds of such transgenic plants was unknown. In this study, we examined the TAG utilization efficiency during seed germination in transgenic Arabidopsis seeds containing several examples of these fatty acids. Seed TAG species with native fatty acids had higher utilization rate than the TAG species containing transgenically produced polyunsaturated fatty acids. Conversely, quantification of the fatty acid components remaining in the total TAG after early stages of seed germination revealed that the undigested TAGs tended to contain an elevated level of the engineered polyunsaturated fatty acids (PUFA). LC-MS analysis further revealed asymmetrical mobilization rates for the individual TAG species. TAGs which contained multiple PUFA fatty acids were mobilized slower than the species containing single PUFA. The mobilised engineered fatty acids were used in de novo membrane lipid synthesis during seedling development.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01402/fullArabidopsislipidomicsDHAseed germinationsdaω3 LC-PUFA |
| spellingShingle | Pushkar Shrestha Damien L Callahan Damien L Callahan Surinder P Singh James Robertson Petrie Xue-Rong Zhou Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids Frontiers in Plant Science Arabidopsis lipidomics DHA seed germination sda ω3 LC-PUFA |
| title | Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids |
| title_full | Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids |
| title_fullStr | Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids |
| title_full_unstemmed | Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids |
| title_short | Reduced triacylglycerol mobilization during seed germination and early seedling growth in Arabidopsis containing nutritionally important polyunsaturated fatty acids |
| title_sort | reduced triacylglycerol mobilization during seed germination and early seedling growth in arabidopsis containing nutritionally important polyunsaturated fatty acids |
| topic | Arabidopsis lipidomics DHA seed germination sda ω3 LC-PUFA |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01402/full |
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