Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach
The official standard for quality control of honey is currently based on physicochemical properties. However, this method is time-consuming, cost intensive, and does not lead to information on the originality of honey. This study aims to classify raw stingless bee honeys by bee species origins as a...
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MDPI AG
2018-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/9/2160 |
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| author | Muhammad Taufiq Atsifa Razali Zaim Akmal Zainal M. Maulidiani Khozirah Shaari Zulkifli Zamri Mohd Zainuri Mohd Idrus Alfi Khatib Faridah Abas Yee Soon Ling Lim Leong Rui Intan Safinar Ismail |
| author_facet | Muhammad Taufiq Atsifa Razali Zaim Akmal Zainal M. Maulidiani Khozirah Shaari Zulkifli Zamri Mohd Zainuri Mohd Idrus Alfi Khatib Faridah Abas Yee Soon Ling Lim Leong Rui Intan Safinar Ismail |
| author_sort | Muhammad Taufiq Atsifa Razali |
| collection | DOAJ |
| description | The official standard for quality control of honey is currently based on physicochemical properties. However, this method is time-consuming, cost intensive, and does not lead to information on the originality of honey. This study aims to classify raw stingless bee honeys by bee species origins as a potential classifier using the NMR-LCMS-based metabolomics approach. Raw stingless bee honeys were analysed and classified by bee species origins using proton nuclear magnetic resonance (1H-NMR) spectroscopy and an ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF MS) in combination with chemometrics tools. The honey samples were able to be classified into three different groups based on the bee species origins of Heterotrigona itama, Geniotrigona thoracica, and Tetrigona apicalis. d-Fructofuranose (H. itama honey), β-d-Glucose, d-Xylose, α-d-Glucose (G. thoracica honey), and l-Lactic acid, Acetic acid, l-Alanine (T. apicalis honey) ident d-Fructofuranose identified via 1H-NMR data and the diagnostic ions of UHPLC-QTOF MS were characterized as the discriminant metabolites or putative chemical markers. It could be suggested that the quality of honey in terms of originality and purity can be rapidly determined using the classification technique by bee species origins via the 1H-NMR- and UHPLC-QTOF MS-based metabolomics approach. |
| first_indexed | 2024-12-11T18:15:06Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T18:15:06Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2c0606bc544e4fee97ebd27595367b7e2022-12-22T00:55:27ZengMDPI AGMolecules1420-30492018-08-01239216010.3390/molecules23092160molecules23092160Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics ApproachMuhammad Taufiq Atsifa Razali0Zaim Akmal Zainal1M. Maulidiani2Khozirah Shaari3Zulkifli Zamri4Mohd Zainuri Mohd Idrus5Alfi Khatib6Faridah Abas7Yee Soon Ling8Lim Leong Rui9Intan Safinar Ismail10Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaSyamille Agrofarm & Resort Sdn. Bhd., Lot 3749 & 3750, Jalan Lata Perahu, Kampung Chuar Hulu, Mukim Chegar Galah, 33020 Kati, Kuala Kangsar, Perak, MalaysiaSyamille Agrofarm & Resort Sdn. Bhd., Lot 3749 & 3750, Jalan Lata Perahu, Kampung Chuar Hulu, Mukim Chegar Galah, 33020 Kati, Kuala Kangsar, Perak, MalaysiaDepartment of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia (IIUM), Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaThe official standard for quality control of honey is currently based on physicochemical properties. However, this method is time-consuming, cost intensive, and does not lead to information on the originality of honey. This study aims to classify raw stingless bee honeys by bee species origins as a potential classifier using the NMR-LCMS-based metabolomics approach. Raw stingless bee honeys were analysed and classified by bee species origins using proton nuclear magnetic resonance (1H-NMR) spectroscopy and an ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF MS) in combination with chemometrics tools. The honey samples were able to be classified into three different groups based on the bee species origins of Heterotrigona itama, Geniotrigona thoracica, and Tetrigona apicalis. d-Fructofuranose (H. itama honey), β-d-Glucose, d-Xylose, α-d-Glucose (G. thoracica honey), and l-Lactic acid, Acetic acid, l-Alanine (T. apicalis honey) ident d-Fructofuranose identified via 1H-NMR data and the diagnostic ions of UHPLC-QTOF MS were characterized as the discriminant metabolites or putative chemical markers. It could be suggested that the quality of honey in terms of originality and purity can be rapidly determined using the classification technique by bee species origins via the 1H-NMR- and UHPLC-QTOF MS-based metabolomics approach.http://www.mdpi.com/1420-3049/23/9/2160classificationstingless bee honeybee species originsmetabolomicsNMRLC-MSchemometrics |
| spellingShingle | Muhammad Taufiq Atsifa Razali Zaim Akmal Zainal M. Maulidiani Khozirah Shaari Zulkifli Zamri Mohd Zainuri Mohd Idrus Alfi Khatib Faridah Abas Yee Soon Ling Lim Leong Rui Intan Safinar Ismail Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach Molecules classification stingless bee honey bee species origins metabolomics NMR LC-MS chemometrics |
| title | Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach |
| title_full | Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach |
| title_fullStr | Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach |
| title_full_unstemmed | Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach |
| title_short | Classification of Raw Stingless Bee Honeys by Bee Species Origins Using the NMR- and LC-MS-Based Metabolomics Approach |
| title_sort | classification of raw stingless bee honeys by bee species origins using the nmr and lc ms based metabolomics approach |
| topic | classification stingless bee honey bee species origins metabolomics NMR LC-MS chemometrics |
| url | http://www.mdpi.com/1420-3049/23/9/2160 |
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