Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples
The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using differe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1266985/full |
| _version_ | 1797663187757170688 |
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| author | Chunyu Lu Di Peng W. C. K. Udeesha Erandani Kimberly Mitchell Christopher J. Martyniuk Vance L. Trudeau |
| author_facet | Chunyu Lu Di Peng W. C. K. Udeesha Erandani Kimberly Mitchell Christopher J. Martyniuk Vance L. Trudeau |
| author_sort | Chunyu Lu |
| collection | DOAJ |
| description | The detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems. |
| first_indexed | 2024-03-11T19:10:55Z |
| format | Article |
| id | doaj.art-2c08269987964be08d6ff3a85f385d0b |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-11T19:10:55Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-2c08269987964be08d6ff3a85f385d0b2023-10-09T13:37:00ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-10-011410.3389/fendo.2023.12669851266985Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samplesChunyu Lu0Di Peng1W. C. K. Udeesha Erandani2Kimberly Mitchell3Christopher J. Martyniuk4Vance L. Trudeau5Department of Biology, University of Ottawa, Ottawa, ON, CanadaDepartment of Biology, University of Ottawa, Ottawa, ON, CanadaDepartment of Biology, University of Ottawa, Ottawa, ON, CanadaDepartment of Biology, University of Ottawa, Ottawa, ON, CanadaDepartment of Physiological Sciences, University of Florida, Gainesville, FL, United StatesDepartment of Biology, University of Ottawa, Ottawa, ON, CanadaThe detection and quantification of hormones are important to assess the reproductive and stress status of experimental models and for the diagnosis of diseases in human and veterinary clinics. Traditionally, steroid, peptide, and protein hormones are analyzed in individual experiments using different extraction methodologies. With the new advancement on HPLC sorbents, the simultaneous measurement of hormones from different categories becomes possible. In this study, we present a novel sample processing strategy for the simultaneous extraction and detection of peptides, steroids, and proteins using high-resolution liquid chromatography tandem mass spectrometry. We demonstrate the sensitivity of our method for small tissues by acquiring data from brain, pituitary gland, and gonads of single zebrafish samples. This approach promises to shed light on the hormonal pathways and their interrelationships, providing knowledge on the integration of hormone systems.https://www.frontiersin.org/articles/10.3389/fendo.2023.1266985/fullprotein measurementpeptide quantificationsteroid detectionLCMS (liquid chromatography-mass spectrometry)solid phase extraction |
| spellingShingle | Chunyu Lu Di Peng W. C. K. Udeesha Erandani Kimberly Mitchell Christopher J. Martyniuk Vance L. Trudeau Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples Frontiers in Endocrinology protein measurement peptide quantification steroid detection LCMS (liquid chromatography-mass spectrometry) solid phase extraction |
| title | Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples |
| title_full | Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples |
| title_fullStr | Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples |
| title_full_unstemmed | Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples |
| title_short | Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples |
| title_sort | simultaneous extraction and detection of peptides steroids and proteins in small tissue samples |
| topic | protein measurement peptide quantification steroid detection LCMS (liquid chromatography-mass spectrometry) solid phase extraction |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1266985/full |
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