Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>
Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/11/648 |
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| author | Guillaume Tanguy Aline Legat Olivier Gonçalves Luc Marchal Benoît Schoefs |
| author_facet | Guillaume Tanguy Aline Legat Olivier Gonçalves Luc Marchal Benoît Schoefs |
| author_sort | Guillaume Tanguy |
| collection | DOAJ |
| description | Biocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga <i>Dunaliella salina</i> using <i>n</i>-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm<sup>3</sup> and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm<sup>3</sup>) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction. |
| first_indexed | 2024-03-10T05:19:30Z |
| format | Article |
| id | doaj.art-2c0a258ff224436a845faf343239af5c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T05:19:30Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-2c0a258ff224436a845faf343239af5c2023-11-23T00:07:49ZengMDPI AGMarine Drugs1660-33972021-11-01191164810.3390/md19110648Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i>Guillaume Tanguy0Aline Legat1Olivier Gonçalves2Luc Marchal3Benoît Schoefs4Laboratoire GEPEA, Université de Nantes, Oniris, UMR 6144, 44600 Saint-Nazaire, FranceLaboratoire GEPEA, Université de Nantes, Oniris, UMR 6144, 44600 Saint-Nazaire, FranceLaboratoire GEPEA, Université de Nantes, Oniris, UMR 6144, 44600 Saint-Nazaire, FranceLaboratoire GEPEA, Université de Nantes, Oniris, UMR 6144, 44600 Saint-Nazaire, FranceMetabolism, Bio-Engineering of Microalgal Molecules and Applications, Laboratoire Mer Molécules Santé, IUML–FR 3473 CNRS, Le Mans Université, Avenue Olivier Messiaen, 72085 Le Mans, FranceBiocompatible extraction emerges recently as a means to reduce costs of biotechnology processing of microalgae. In this frame, this study aimed at determining how specific culture conditions and the associated cell morphology impact the biocompatibility and the extraction yield of β-carotene from the green microalga <i>Dunaliella salina</i> using <i>n</i>-decane. The results highlight the relationship between the cell disruption yield and cell volume, the circularity and the relative abundance of naturally permeabilized cells. The disruption rate increased with both the cell volume and circularity. This was particularly obvious for volume and circularity exceeding 1500 µm<sup>3</sup> and 0.7, respectively. The extraction of β-carotene was the most biocompatible with small (600 µm<sup>3</sup>) and circular cells (0.7) stressed in photobioreactor (30% of carotenoids recovery with 15% cell disruption). The naturally permeabilized cells were disrupted first; the remaining cells seems to follow a gradual permeabilization process: reversibility (up to 20 s) then irreversibility and cell disruption. This opens new carotenoid production schemes based on growing robust β-carotene enriched cells to ensure biocompatible extraction.https://www.mdpi.com/1660-3397/19/11/648microalgae<i>Dunaliella salina</i>β-carotenein situ extractionmilkingbiocompatibility |
| spellingShingle | Guillaume Tanguy Aline Legat Olivier Gonçalves Luc Marchal Benoît Schoefs Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i> Marine Drugs microalgae <i>Dunaliella salina</i> β-carotene in situ extraction milking biocompatibility |
| title | Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i> |
| title_full | Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i> |
| title_fullStr | Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i> |
| title_full_unstemmed | Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i> |
| title_short | Selection of Culture Conditions and Cell Morphology for Biocompatible Extraction of β-Carotene from <i>Dunaliella salina</i> |
| title_sort | selection of culture conditions and cell morphology for biocompatible extraction of β carotene from i dunaliella salina i |
| topic | microalgae <i>Dunaliella salina</i> β-carotene in situ extraction milking biocompatibility |
| url | https://www.mdpi.com/1660-3397/19/11/648 |
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