Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali
Malus sieversii is the wild progenitor for many cultivars of domesticated apple and an important germplasm resource for breeding. However, this valuable species faces a significant threat in the areas north of the Tianshan Mountains in China, by the invasion of Agrilus mali, a destructive pest of ap...
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PeerJ Inc.
2020-05-01
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| Online Access: | https://peerj.com/articles/8992.pdf |
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| author | Chuang Mei Jie Yang Peng Yan Ning Li Kai Ma Aisajan Mamat Liqun Han Qinglong Dong Ke Mao Fengwang Ma Jixun Wang |
| author_facet | Chuang Mei Jie Yang Peng Yan Ning Li Kai Ma Aisajan Mamat Liqun Han Qinglong Dong Ke Mao Fengwang Ma Jixun Wang |
| author_sort | Chuang Mei |
| collection | DOAJ |
| description | Malus sieversii is the wild progenitor for many cultivars of domesticated apple and an important germplasm resource for breeding. However, this valuable species faces a significant threat in the areas north of the Tianshan Mountains in China, by the invasion of Agrilus mali, a destructive pest of apple trees belonging to the family Buprestidae. Our preliminary study has has shown that there may be resistance to this insect in M. sieversii plants in the field, but the corresponding molecular mechanisms remain unclear. In this study, we compared the response of insect-resistant and insect-susceptible plants of M. sieversii to insect feeding using full-length transcriptome and targeted metabolome. 112,103 non-chimeric full-length reads (FLNC) totaling 10.52 Gb of data were generating with Pacific Biosciences SingleMolecule, Real-Time (PacBio SMRT) sequencing. A total of 130.06 Gb data of long reads were acquired with an Illumina HiSeq. Function annotation indicated that the different expressed genes (DEGs) were mainly involved in signal transduction pathway of plant hormones and in the synthesis of compounds such as terpenes, quinones, flavonoids, and jasmonic acid. Through targeted metabolome analysis resistant strains showed higher levels of trans-cinnamic acid, caffeine and ferulic acid after pest infestation. This study helps to decipher the transcriptional changes and related signaling paths in M. sieversii after an insect feeding, which lays a foundation for further research on molecular mechanisms of insect resistance in apples. |
| first_indexed | 2024-03-09T07:06:04Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:06:04Z |
| publishDate | 2020-05-01 |
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| spelling | doaj.art-2c0bd0430bee4967b96a120dcc3730082023-12-03T09:31:09ZengPeerJ Inc.PeerJ2167-83592020-05-018e899210.7717/peerj.8992Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus maliChuang Mei0Jie Yang1Peng Yan2Ning Li3Kai Ma4Aisajan Mamat5Liqun Han6Qinglong Dong7Ke Mao8Fengwang Ma9Jixun Wang10State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling, Shaanxi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling, Shaanxi, ChinaInstitute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi, ChinaMalus sieversii is the wild progenitor for many cultivars of domesticated apple and an important germplasm resource for breeding. However, this valuable species faces a significant threat in the areas north of the Tianshan Mountains in China, by the invasion of Agrilus mali, a destructive pest of apple trees belonging to the family Buprestidae. Our preliminary study has has shown that there may be resistance to this insect in M. sieversii plants in the field, but the corresponding molecular mechanisms remain unclear. In this study, we compared the response of insect-resistant and insect-susceptible plants of M. sieversii to insect feeding using full-length transcriptome and targeted metabolome. 112,103 non-chimeric full-length reads (FLNC) totaling 10.52 Gb of data were generating with Pacific Biosciences SingleMolecule, Real-Time (PacBio SMRT) sequencing. A total of 130.06 Gb data of long reads were acquired with an Illumina HiSeq. Function annotation indicated that the different expressed genes (DEGs) were mainly involved in signal transduction pathway of plant hormones and in the synthesis of compounds such as terpenes, quinones, flavonoids, and jasmonic acid. Through targeted metabolome analysis resistant strains showed higher levels of trans-cinnamic acid, caffeine and ferulic acid after pest infestation. This study helps to decipher the transcriptional changes and related signaling paths in M. sieversii after an insect feeding, which lays a foundation for further research on molecular mechanisms of insect resistance in apples.https://peerj.com/articles/8992.pdfMalus sieversiiAgrilus maliGene discoveryFull-length transcriptomeInsect resistance |
| spellingShingle | Chuang Mei Jie Yang Peng Yan Ning Li Kai Ma Aisajan Mamat Liqun Han Qinglong Dong Ke Mao Fengwang Ma Jixun Wang Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali PeerJ Malus sieversii Agrilus mali Gene discovery Full-length transcriptome Insect resistance |
| title | Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali |
| title_full | Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali |
| title_fullStr | Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali |
| title_full_unstemmed | Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali |
| title_short | Full-length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of Malus sieversii against Agrilus mali |
| title_sort | full length transcriptome and targeted metabolome analyses provide insights into defense mechanisms of malus sieversii against agrilus mali |
| topic | Malus sieversii Agrilus mali Gene discovery Full-length transcriptome Insect resistance |
| url | https://peerj.com/articles/8992.pdf |
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