Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages
Wound healing is a complex biological phenomenon, having different but overlapping stages to obtained complete re-epithelization. The aim of the current study was to develop a dendrimer-based hydrogel bandage, to ameliorate full-thickness wounds. Hesperidin, a bioflavonoid found in vegetables and ci...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/7/462 |
| _version_ | 1797440523297882112 |
|---|---|
| author | Praveen Gupta Afsana Sheikh Mohammed A. S. Abourehab Prashant Kesharwani |
| author_facet | Praveen Gupta Afsana Sheikh Mohammed A. S. Abourehab Prashant Kesharwani |
| author_sort | Praveen Gupta |
| collection | DOAJ |
| description | Wound healing is a complex biological phenomenon, having different but overlapping stages to obtained complete re-epithelization. The aim of the current study was to develop a dendrimer-based hydrogel bandage, to ameliorate full-thickness wounds. Hesperidin, a bioflavonoid found in vegetables and citrus fruits, is used for treatment of wounds; however, its therapeutic use is limited, due to poor water solubility and poor bioavailability. This issue was overcome by incorporating hesperidin in the inner core of a dendrimer. Hence, a dendrimer-based hydrogel bandage was prepared, and the wound healing activity was determined. A hemolysis study indicated that the hesperidin-loaded dendrimer was biocompatible and can be used for wound healing. The therapeutic efficacy of the prepared formulation was evaluated on a full-thickness wound, using an animal model. H&E staining of the control group showed degenerated neutrophils and eosinophils, while 10% of the formulation showed wound closure, formation of the epidermal layer, and remodeling. The MT staining of the 10% formulation showed better collagen synthesis compared to the control group. In vivo results showed that the preparation had better wound contraction activity compared to the control group; after 14 days, the control group had 79 ± 1.41, while the 10% of formulation had 98.9 ± 0.42. In a nutshell, Hsp-P-Hyd 10% showed the best overall performance in amelioration of full-thickness wounds. |
| first_indexed | 2024-03-09T12:10:23Z |
| format | Article |
| id | doaj.art-2c0d2a0b5ed6467f9e5b043a91e5203a |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T12:10:23Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-2c0d2a0b5ed6467f9e5b043a91e5203a2023-11-30T22:53:02ZengMDPI AGBiosensors2079-63742022-06-0112746210.3390/bios12070462Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel BandagesPraveen Gupta0Afsana Sheikh1Mohammed A. S. Abourehab2Prashant Kesharwani3Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Pharmaceutics, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, IndiaWound healing is a complex biological phenomenon, having different but overlapping stages to obtained complete re-epithelization. The aim of the current study was to develop a dendrimer-based hydrogel bandage, to ameliorate full-thickness wounds. Hesperidin, a bioflavonoid found in vegetables and citrus fruits, is used for treatment of wounds; however, its therapeutic use is limited, due to poor water solubility and poor bioavailability. This issue was overcome by incorporating hesperidin in the inner core of a dendrimer. Hence, a dendrimer-based hydrogel bandage was prepared, and the wound healing activity was determined. A hemolysis study indicated that the hesperidin-loaded dendrimer was biocompatible and can be used for wound healing. The therapeutic efficacy of the prepared formulation was evaluated on a full-thickness wound, using an animal model. H&E staining of the control group showed degenerated neutrophils and eosinophils, while 10% of the formulation showed wound closure, formation of the epidermal layer, and remodeling. The MT staining of the 10% formulation showed better collagen synthesis compared to the control group. In vivo results showed that the preparation had better wound contraction activity compared to the control group; after 14 days, the control group had 79 ± 1.41, while the 10% of formulation had 98.9 ± 0.42. In a nutshell, Hsp-P-Hyd 10% showed the best overall performance in amelioration of full-thickness wounds.https://www.mdpi.com/2079-6374/12/7/462wound healingdendrimerhydrogelbandagefull-thickness woundnanotechnology |
| spellingShingle | Praveen Gupta Afsana Sheikh Mohammed A. S. Abourehab Prashant Kesharwani Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages Biosensors wound healing dendrimer hydrogel bandage full-thickness wound nanotechnology |
| title | Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages |
| title_full | Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages |
| title_fullStr | Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages |
| title_full_unstemmed | Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages |
| title_short | Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages |
| title_sort | amelioration of full thickness wound using hesperidin loaded dendrimer based hydrogel bandages |
| topic | wound healing dendrimer hydrogel bandage full-thickness wound nanotechnology |
| url | https://www.mdpi.com/2079-6374/12/7/462 |
| work_keys_str_mv | AT praveengupta ameliorationoffullthicknesswoundusinghesperidinloadeddendrimerbasedhydrogelbandages AT afsanasheikh ameliorationoffullthicknesswoundusinghesperidinloadeddendrimerbasedhydrogelbandages AT mohammedasabourehab ameliorationoffullthicknesswoundusinghesperidinloadeddendrimerbasedhydrogelbandages AT prashantkesharwani ameliorationoffullthicknesswoundusinghesperidinloadeddendrimerbasedhydrogelbandages |