Use of Agrobacterium rhizogenes strain 18r12v and paromomycin selection for transformation of Brachypodium distachyon and Brachypodium sylvaticum

The genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brach...

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Bibliographic Details
Main Authors: Ray eCollier, Jennifer eBragg, Bryan Tarape Hernandez, John P. Vogel, Roger eThilmony
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-05-01
Series:Frontiers in Plant Science
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00716/full