Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort

A renaissance in cell-free protein synthesis (CFPS) is underway, enabled by the acceleration and adoption of synthetic biology methods. CFPS has emerged as a powerful platform technology for synthetic gene network design, biosensing and on-demand biomanufacturing. Whilst primarily of bacterial origi...

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Bibliographic Details
Main Authors: Alex J. Spice, Rochelle Aw, Daniel G. Bracewell, Karen M. Polizzi
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-09-01
Series:Synthetic and Systems Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X20300302