Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort
A renaissance in cell-free protein synthesis (CFPS) is underway, enabled by the acceleration and adoption of synthetic biology methods. CFPS has emerged as a powerful platform technology for synthetic gene network design, biosensing and on-demand biomanufacturing. Whilst primarily of bacterial origi...
Main Authors: | Alex J. Spice, Rochelle Aw, Daniel G. Bracewell, Karen M. Polizzi |
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Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2020-09-01
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Series: | Synthetic and Systems Biotechnology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X20300302 |
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