Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation
Immunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on the market are ferrite coated with a silicon shell. Based on a new type of magnetic material, the graphene coated iron nitride magnetic particle (G@FeN-MP), which we previously...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Magnetochemistry |
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| Online Access: | https://www.mdpi.com/2312-7481/7/5/58 |
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| author | Tianya Liang Jianxing Li Xiao Liu Zhuang Ma Xiaojin Su Xiangjiao Meng Ziyi Zhanghuang Huiqin Wang Jintao Li Qun Wang Minglian Wang |
| author_facet | Tianya Liang Jianxing Li Xiao Liu Zhuang Ma Xiaojin Su Xiangjiao Meng Ziyi Zhanghuang Huiqin Wang Jintao Li Qun Wang Minglian Wang |
| author_sort | Tianya Liang |
| collection | DOAJ |
| description | Immunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on the market are ferrite coated with a silicon shell. Based on a new type of magnetic material, the graphene coated iron nitride magnetic particle (G@FeN-MP), which we previously reported, we prepared a novel IMB, a graphene oxide coated iron nitride immune magnetic bead (GO@FeN-IMBs), and explored its feasibility for cell sorting. First, the surface of the G@FeN-MP was oxidized to produce oxygen-containing groups as carboxyl, etc. by the optimized Hummers’ method, followed by a homogenization procedure to make the particles uniform in size and dispersive. The carboxy groups generated were then condensed and coupled with anti-CD3 antibodies by the carbodiimide method to produce an anti-CD3-GO@FeN-IMB after the coupling efficacy was proved by bovine serum albumin (BSA) and labeled antibodies. Finally, the anti-CD3-GO@FeN-IMBs were incubated with a cell mixture containing human T cells. With the aid of a magnetic stand, the T cells were successfully isolated from the cell mixture. The isolated T cells turned out to be intact and could proliferate with the activation of the IMBs. The results show that the G@FeN-MP can be modified for IMB preparation, and the anti-CD3-GO@FeN-IMBs we prepared can potentially separate T cells. |
| first_indexed | 2024-03-10T11:48:54Z |
| format | Article |
| id | doaj.art-2c15bb8913174f5f864ba2dd9ad32af8 |
| institution | Directory Open Access Journal |
| issn | 2312-7481 |
| language | English |
| last_indexed | 2024-03-10T11:48:54Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Magnetochemistry |
| spelling | doaj.art-2c15bb8913174f5f864ba2dd9ad32af82023-11-21T17:51:52ZengMDPI AGMagnetochemistry2312-74812021-04-01755810.3390/magnetochemistry7050058Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell SeparationTianya Liang0Jianxing Li1Xiao Liu2Zhuang Ma3Xiaojin Su4Xiangjiao Meng5Ziyi Zhanghuang6Huiqin Wang7Jintao Li8Qun Wang9Minglian Wang10Faculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Material and Manufacture Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Material and Manufacture Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Material and Manufacture Sciences, Beijing University of Technology, Beijing 100124, ChinaFaculty of Environmental and Life Sciences, Beijing University of Technology, Beijing 100124, ChinaImmunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on the market are ferrite coated with a silicon shell. Based on a new type of magnetic material, the graphene coated iron nitride magnetic particle (G@FeN-MP), which we previously reported, we prepared a novel IMB, a graphene oxide coated iron nitride immune magnetic bead (GO@FeN-IMBs), and explored its feasibility for cell sorting. First, the surface of the G@FeN-MP was oxidized to produce oxygen-containing groups as carboxyl, etc. by the optimized Hummers’ method, followed by a homogenization procedure to make the particles uniform in size and dispersive. The carboxy groups generated were then condensed and coupled with anti-CD3 antibodies by the carbodiimide method to produce an anti-CD3-GO@FeN-IMB after the coupling efficacy was proved by bovine serum albumin (BSA) and labeled antibodies. Finally, the anti-CD3-GO@FeN-IMBs were incubated with a cell mixture containing human T cells. With the aid of a magnetic stand, the T cells were successfully isolated from the cell mixture. The isolated T cells turned out to be intact and could proliferate with the activation of the IMBs. The results show that the G@FeN-MP can be modified for IMB preparation, and the anti-CD3-GO@FeN-IMBs we prepared can potentially separate T cells.https://www.mdpi.com/2312-7481/7/5/58graphene oxideiron nitride magnetic particlesT cell separationimmune magnetic beads |
| spellingShingle | Tianya Liang Jianxing Li Xiao Liu Zhuang Ma Xiaojin Su Xiangjiao Meng Ziyi Zhanghuang Huiqin Wang Jintao Li Qun Wang Minglian Wang Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation Magnetochemistry graphene oxide iron nitride magnetic particles T cell separation immune magnetic beads |
| title | Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation |
| title_full | Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation |
| title_fullStr | Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation |
| title_full_unstemmed | Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation |
| title_short | Preparation of CD3 Antibody-Conjugated, Graphene Oxide Coated Iron Nitride Magnetic Beads and Its Preliminary Application in T Cell Separation |
| title_sort | preparation of cd3 antibody conjugated graphene oxide coated iron nitride magnetic beads and its preliminary application in t cell separation |
| topic | graphene oxide iron nitride magnetic particles T cell separation immune magnetic beads |
| url | https://www.mdpi.com/2312-7481/7/5/58 |
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