Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis
Many bioactive inorganic ions such as calcium (Ca), magnesium (Mg) and silicon (Si) have been confirmed to take part in bone regeneration through promoting the secretion of osteogenesis-related growth factors. However, it is not clear about the effective concentration range and action mechanism of i...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-01-01
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| Series: | Smart Materials in Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590183421000259 |
| _version_ | 1811173268254621696 |
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| author | Hong Lan Mengjiao Zhang Xianchun Chen Zhongbing Huang Guangfu Yin |
| author_facet | Hong Lan Mengjiao Zhang Xianchun Chen Zhongbing Huang Guangfu Yin |
| author_sort | Hong Lan |
| collection | DOAJ |
| description | Many bioactive inorganic ions such as calcium (Ca), magnesium (Mg) and silicon (Si) have been confirmed to take part in bone regeneration through promoting the secretion of osteogenesis-related growth factors. However, it is not clear about the effective concentration range and action mechanism of ion combinations on synergistically facilitating osteogenesis. Besides, it is of great significance to design a novel bioceramic that could continuously release these effective inorganic ions to match the need for bone tissue repair. Thus, in this work, the concentration level of combined Ca, Mg, Si ions beneficial to osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) was explored by orthogonal experimental design (OED) firstly. Meanwhile, eight single-phase ceramics in CaO–MgO–SiO2 system were synthesized, and the influences of their inherent properties on ion dissolution behaviors were systematically investigated. Finally, based on the above results, three multiphase bioceramics (named C1, C2 and C3) containing calcium silicate, diopside and akermanite as crystalline phase were designed and synthesized. It was found that the ionic extracts of bioceramic C1 were just within the above effective concentration range, displaying a predetermined ability of stimulating osteogenic differentiation of BMSCs through enhancing the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF). All the results indicated that this novel multiphase bioceramic with adjustable ion dissolution behavior has a great application potential in bone tissue engineering. |
| first_indexed | 2024-04-10T17:45:07Z |
| format | Article |
| id | doaj.art-2c17edc28e334d67aa70b327e225857d |
| institution | Directory Open Access Journal |
| issn | 2590-1834 |
| language | English |
| last_indexed | 2024-04-10T17:45:07Z |
| publishDate | 2022-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Smart Materials in Medicine |
| spelling | doaj.art-2c17edc28e334d67aa70b327e225857d2023-02-03T05:00:57ZengKeAi Communications Co., Ltd.Smart Materials in Medicine2590-18342022-01-01394103Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesisHong Lan0Mengjiao Zhang1Xianchun Chen2Zhongbing Huang3Guangfu Yin4College of Biomedical Engineering, Sichuan University, Chengdu, 610064, PR ChinaKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, West China School of Pharmacy, Sichuan University, Chengdu, 610041, ChinaCollege of Polymer Science and Engineering, Sichuan University, Chengdu, 610064, PR China; Corresponding author. No.24, South 1st Section, 1st Ring Road, Chengdu, Sichuan, 610064, China.College of Biomedical Engineering, Sichuan University, Chengdu, 610064, PR ChinaCollege of Biomedical Engineering, Sichuan University, Chengdu, 610064, PR ChinaMany bioactive inorganic ions such as calcium (Ca), magnesium (Mg) and silicon (Si) have been confirmed to take part in bone regeneration through promoting the secretion of osteogenesis-related growth factors. However, it is not clear about the effective concentration range and action mechanism of ion combinations on synergistically facilitating osteogenesis. Besides, it is of great significance to design a novel bioceramic that could continuously release these effective inorganic ions to match the need for bone tissue repair. Thus, in this work, the concentration level of combined Ca, Mg, Si ions beneficial to osteogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) was explored by orthogonal experimental design (OED) firstly. Meanwhile, eight single-phase ceramics in CaO–MgO–SiO2 system were synthesized, and the influences of their inherent properties on ion dissolution behaviors were systematically investigated. Finally, based on the above results, three multiphase bioceramics (named C1, C2 and C3) containing calcium silicate, diopside and akermanite as crystalline phase were designed and synthesized. It was found that the ionic extracts of bioceramic C1 were just within the above effective concentration range, displaying a predetermined ability of stimulating osteogenic differentiation of BMSCs through enhancing the expression of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF). All the results indicated that this novel multiphase bioceramic with adjustable ion dissolution behavior has a great application potential in bone tissue engineering.http://www.sciencedirect.com/science/article/pii/S2590183421000259Synergistic effectCaO–MgO–SiO2-based multiphase bioceramicIon dissolution behaviorOsteogenic differentiationGrowth factor |
| spellingShingle | Hong Lan Mengjiao Zhang Xianchun Chen Zhongbing Huang Guangfu Yin Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis Smart Materials in Medicine Synergistic effect CaO–MgO–SiO2-based multiphase bioceramic Ion dissolution behavior Osteogenic differentiation Growth factor |
| title | Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis |
| title_full | Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis |
| title_fullStr | Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis |
| title_full_unstemmed | Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis |
| title_short | Designing a novel CaO–MgO–SiO2-based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis |
| title_sort | designing a novel cao mgo sio2 based multiphase bioceramic with adjustable ion dissolution behavior for enhancing osteogenesis |
| topic | Synergistic effect CaO–MgO–SiO2-based multiphase bioceramic Ion dissolution behavior Osteogenic differentiation Growth factor |
| url | http://www.sciencedirect.com/science/article/pii/S2590183421000259 |
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