Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance
Soil salinization poses a serious threat to the environment and agricultural productivity worldwide. Studies on the physiological and molecular mechanisms of salinity tolerance in halophytic plants provide valuable information to enhance their salt tolerance. Tangut Nitraria is a widely distributed...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00030/full |
| _version_ | 1819137187491348480 |
|---|---|
| author | Tielong eCheng Tielong eCheng Jinhui eChen Jingbo eZhang Shengqing eShi Yanwei eZhou Lu eLu Pengkai eWang Zeping eJiang Jinchang eYang Shougong eZhang Jisen eShi |
| author_facet | Tielong eCheng Tielong eCheng Jinhui eChen Jingbo eZhang Shengqing eShi Yanwei eZhou Lu eLu Pengkai eWang Zeping eJiang Jinchang eYang Shougong eZhang Jisen eShi |
| author_sort | Tielong eCheng |
| collection | DOAJ |
| description | Soil salinization poses a serious threat to the environment and agricultural productivity worldwide. Studies on the physiological and molecular mechanisms of salinity tolerance in halophytic plants provide valuable information to enhance their salt tolerance. Tangut Nitraria is a widely distributed halophyte in saline–alkali soil in the northern areas of China. In this study, we used a proteomic approach to investigate the molecular pathways of the high salt tolerance of T. Nitraria. We analyzed the changes in biomass, photosynthesis, and redox-related enzyme activities in T. Nitraria leaves from plant seedlings treated with high salt concentration. Comparative proteomic analysis of the leaves revealed that the expression of 71 proteins was significantly altered after salinity treatments of T. Nitraria. These salinity-responsive proteins were mainly involved in photosynthesis, redox homeostasis, stress/defense, carbohydrate and energy metabolism, protein metabolism, signal transduction, and membrane transport. Results showed that the reduction of photosynthesis under salt stress was attributed to the down-regulation of the enzymes and proteins involved in the light reaction and Calvin cycle. Protein–protein interaction analysis revealed that the proteins involved in redox homeostasis, photosynthesis, and energy metabolism constructed two types of response networks to high salt stress. T. Nitraria plants developed diverse mechanisms for scavenging reactive oxygen species in their leaves to cope with stress induced by high salinity. This study provides important information regarding the salt tolerance of the halophyte T. Nitraria. |
| first_indexed | 2024-12-22T10:46:53Z |
| format | Article |
| id | doaj.art-2c1be40e6b544dbcbc9f82907e5e50b1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T10:46:53Z |
| publishDate | 2015-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2c1be40e6b544dbcbc9f82907e5e50b12022-12-21T18:28:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-02-01610.3389/fpls.2015.00030121614Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity toleranceTielong eCheng0Tielong eCheng1Jinhui eChen2Jingbo eZhang3Shengqing eShi4Yanwei eZhou5Lu eLu6Pengkai eWang7Zeping eJiang8Jinchang eYang9Shougong eZhang10Jisen eShi11Nanjing Forestry UniversityChinese Academy of ForestryNanjing Forestry UniversityChinese Academy of ForestryChinese Academy of ForestryNanjing Forestry UniversityNanjing Forestry UniversityNanjing Forestry UniversityChinese Academy of ForestryResearch Institute of Tropical Forestry, Chinese Academy Of ForestryChinese Academy of ForestryNanjing Forestry UniversitySoil salinization poses a serious threat to the environment and agricultural productivity worldwide. Studies on the physiological and molecular mechanisms of salinity tolerance in halophytic plants provide valuable information to enhance their salt tolerance. Tangut Nitraria is a widely distributed halophyte in saline–alkali soil in the northern areas of China. In this study, we used a proteomic approach to investigate the molecular pathways of the high salt tolerance of T. Nitraria. We analyzed the changes in biomass, photosynthesis, and redox-related enzyme activities in T. Nitraria leaves from plant seedlings treated with high salt concentration. Comparative proteomic analysis of the leaves revealed that the expression of 71 proteins was significantly altered after salinity treatments of T. Nitraria. These salinity-responsive proteins were mainly involved in photosynthesis, redox homeostasis, stress/defense, carbohydrate and energy metabolism, protein metabolism, signal transduction, and membrane transport. Results showed that the reduction of photosynthesis under salt stress was attributed to the down-regulation of the enzymes and proteins involved in the light reaction and Calvin cycle. Protein–protein interaction analysis revealed that the proteins involved in redox homeostasis, photosynthesis, and energy metabolism constructed two types of response networks to high salt stress. T. Nitraria plants developed diverse mechanisms for scavenging reactive oxygen species in their leaves to cope with stress induced by high salinity. This study provides important information regarding the salt tolerance of the halophyte T. Nitraria.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00030/fulliTRAQhalophytesalinity toleranceTangut NitrariaResponsive pathways |
| spellingShingle | Tielong eCheng Tielong eCheng Jinhui eChen Jingbo eZhang Shengqing eShi Yanwei eZhou Lu eLu Pengkai eWang Zeping eJiang Jinchang eYang Shougong eZhang Jisen eShi Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance Frontiers in Plant Science iTRAQ halophyte salinity tolerance Tangut Nitraria Responsive pathways |
| title | Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance |
| title_full | Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance |
| title_fullStr | Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance |
| title_full_unstemmed | Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance |
| title_short | Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance |
| title_sort | physiological and proteomic analyses of leaves from the halophyte tangut nitraria reveals diverse response pathways critical for high salinity tolerance |
| topic | iTRAQ halophyte salinity tolerance Tangut Nitraria Responsive pathways |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00030/full |
| work_keys_str_mv | AT tielongecheng physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT tielongecheng physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT jinhuiechen physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT jingboezhang physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT shengqingeshi physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT yanweiezhou physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT luelu physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT pengkaiewang physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT zepingejiang physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT jinchangeyang physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT shougongezhang physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance AT jiseneshi physiologicalandproteomicanalysesofleavesfromthehalophytetangutnitrariarevealsdiverseresponsepathwayscriticalforhighsalinitytolerance |