A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing
Objective To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterize...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2020.1773067 |
| _version_ | 1818940963585785856 |
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| author | Roland Wirth Gergely Maróti Róbert Mihók Donát Simon-Fiala Márk Antal Bernadett Pap Anett Demcsák Janos Minarovits Kornél L. Kovács |
| author_facet | Roland Wirth Gergely Maróti Róbert Mihók Donát Simon-Fiala Márk Antal Bernadett Pap Anett Demcsák Janos Minarovits Kornél L. Kovács |
| author_sort | Roland Wirth |
| collection | DOAJ |
| description | Objective To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterized. Methods Shotgun metagenome sequencing of salivary DNA samples from 22 individuals (11 non-smokers and 11 current smokers) was performed using the Ion Torrent PGMTM platform. Quality-filtered reads were analysed by both alignment-based sequence similarity searches and genome-centric binning. Results Prevotella, Veillonella and Streptococcus were the predominant genera in the saliva of both groups. Although the overall composition and diversity of the microbiota were similar, Prevotella was significantly more abundant in salivary samples of current smokers compared to non-smokers. Members of the genus Prevotella were implicated in the development of inflammatory diseases and oral cancer. The abundance of the genus Megasphaera also increased in current smokers, whereas the genera Neisseria, Oribacterium, Capnocytophaga and Porphyromonas were significantly reduced. The data generated by read-based taxonomic classification and genome-centric binning mutually validated the two distinct metagenomic approaches. Conclusion Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development. |
| first_indexed | 2024-12-20T06:48:00Z |
| format | Article |
| id | doaj.art-2c1ddbe7066a4ef995ee3943613fe158 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-20T06:48:00Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-2c1ddbe7066a4ef995ee3943613fe1582022-12-21T19:49:38ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972020-01-0112110.1080/20002297.2020.17730671773067A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencingRoland Wirth0Gergely Maróti1Róbert Mihók2Donát Simon-Fiala3Márk Antal4Bernadett Pap5Anett Demcsák6Janos Minarovits7Kornél L. Kovács8University of SzegedInstitute of Plant Biology, Biological Research CenterUniversity of SzegedUniversity of SzegedUniversity of SzegedInstitute of Plant Biology, Biological Research CenterUniversity of SzegedUniversity of SzegedUniversity of SzegedObjective To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterized. Methods Shotgun metagenome sequencing of salivary DNA samples from 22 individuals (11 non-smokers and 11 current smokers) was performed using the Ion Torrent PGMTM platform. Quality-filtered reads were analysed by both alignment-based sequence similarity searches and genome-centric binning. Results Prevotella, Veillonella and Streptococcus were the predominant genera in the saliva of both groups. Although the overall composition and diversity of the microbiota were similar, Prevotella was significantly more abundant in salivary samples of current smokers compared to non-smokers. Members of the genus Prevotella were implicated in the development of inflammatory diseases and oral cancer. The abundance of the genus Megasphaera also increased in current smokers, whereas the genera Neisseria, Oribacterium, Capnocytophaga and Porphyromonas were significantly reduced. The data generated by read-based taxonomic classification and genome-centric binning mutually validated the two distinct metagenomic approaches. Conclusion Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development.http://dx.doi.org/10.1080/20002297.2020.1773067smokingsaliva metagenomeread-based taxonomygenome-centric binningprevotellamegasphaeraoral cancer |
| spellingShingle | Roland Wirth Gergely Maróti Róbert Mihók Donát Simon-Fiala Márk Antal Bernadett Pap Anett Demcsák Janos Minarovits Kornél L. Kovács A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing Journal of Oral Microbiology smoking saliva metagenome read-based taxonomy genome-centric binning prevotella megasphaera oral cancer |
| title | A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing |
| title_full | A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing |
| title_fullStr | A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing |
| title_full_unstemmed | A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing |
| title_short | A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing |
| title_sort | case study of salivary microbiome in smokers and non smokers in hungary analysis by shotgun metagenome sequencing |
| topic | smoking saliva metagenome read-based taxonomy genome-centric binning prevotella megasphaera oral cancer |
| url | http://dx.doi.org/10.1080/20002297.2020.1773067 |
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