NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE)
Objective of research: To study the activities and properties of nucleoside- diphosphatase (NDPase) in cestode Bothriocephalus scorpii.Materials and methods: Cestodes were homogenized with 10 vol. of extraction medium. NDPase was detected in mitochondria and microsomes with substrates (IDP, GDP, UDP...
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| Format: | Article |
| Language: | Russian |
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Federal Scientific Centre VIEV
2016-12-01
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| Series: | Российский паразитологический журнал |
| Subjects: | |
| Online Access: | https://vniigis.elpub.ru/jour/article/view/312 |
| _version_ | 1797270475267637248 |
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| author | E. A. Burenina |
| author_facet | E. A. Burenina |
| author_sort | E. A. Burenina |
| collection | DOAJ |
| description | Objective of research: To study the activities and properties of nucleoside- diphosphatase (NDPase) in cestode Bothriocephalus scorpii.Materials and methods: Cestodes were homogenized with 10 vol. of extraction medium. NDPase was detected in mitochondria and microsomes with substrates (IDP, GDP, UDP). Inorganic phosphorus was determined by the method of Kochetov (1980). The effects of 10 anthelmintic drugs on the activity of NDPase were studied.Results and discussion: It was found that the mitochondrial and microsomal fractions of cestodes B. scorpii have nucleoside diphosphatase activity.The activity of nucleoside diphosphatase depends on substrates and Mg2+ ions. The impact of various effectors and ions (Ca2+, Mn2+, Zn2+) on enzyme activity was determined.Effects of 10 anthelmintic drugs on activity of nucleoside diphosphatase were studied. The anthelmintics Bitionol and Trichlorophen have been proved effective. |
| first_indexed | 2024-03-08T21:40:55Z |
| format | Article |
| id | doaj.art-2c27be01ecb74c15bf0dcef03001ec8d |
| institution | Directory Open Access Journal |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| last_indexed | 2024-04-25T02:04:51Z |
| publishDate | 2016-12-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj.art-2c27be01ecb74c15bf0dcef03001ec8d2024-03-07T14:34:29ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432016-12-0138452753210.12737/23078312NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE)E. A. Burenina0Institute of Biology and Soil Sciences, Far East Branch of RASObjective of research: To study the activities and properties of nucleoside- diphosphatase (NDPase) in cestode Bothriocephalus scorpii.Materials and methods: Cestodes were homogenized with 10 vol. of extraction medium. NDPase was detected in mitochondria and microsomes with substrates (IDP, GDP, UDP). Inorganic phosphorus was determined by the method of Kochetov (1980). The effects of 10 anthelmintic drugs on the activity of NDPase were studied.Results and discussion: It was found that the mitochondrial and microsomal fractions of cestodes B. scorpii have nucleoside diphosphatase activity.The activity of nucleoside diphosphatase depends on substrates and Mg2+ ions. The impact of various effectors and ions (Ca2+, Mn2+, Zn2+) on enzyme activity was determined.Effects of 10 anthelmintic drugs on activity of nucleoside diphosphatase were studied. The anthelmintics Bitionol and Trichlorophen have been proved effective.https://vniigis.elpub.ru/jour/article/view/312nucleoside-diphosphataseinosine diphosphateuridine diphosphateguanosine diphosphatecestodemitochondriamicrosomesanthelmintic drugs |
| spellingShingle | E. A. Burenina NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE) Российский паразитологический журнал nucleoside-diphosphatase inosine diphosphate uridine diphosphate guanosine diphosphate cestode mitochondria microsomes anthelmintic drugs |
| title | NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE) |
| title_full | NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE) |
| title_fullStr | NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE) |
| title_full_unstemmed | NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE) |
| title_short | NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE) |
| title_sort | nucleoside diphosphatase of cestode bothriocephalus scorpii cestoda bothriocephalidae |
| topic | nucleoside-diphosphatase inosine diphosphate uridine diphosphate guanosine diphosphate cestode mitochondria microsomes anthelmintic drugs |
| url | https://vniigis.elpub.ru/jour/article/view/312 |
| work_keys_str_mv | AT eaburenina nucleosidediphosphataseofcestodebothriocephalusscorpiicestodabothriocephalidae |