Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L.
Hibiscus sabdariffa L. is a naturalized medicinal species in Brazil commonly called a “vinagreira” and is a member of the Malvaceae Juss. family, which has a rich potential of bioactive compounds presenting extracts with antioxidant, antibacterial, anti-inflammatory, hepatoprotective, antiviral, ant...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535222004191 |
| _version_ | 1811294178822324224 |
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| author | Alessandra Carla Guimarães Sobrinho Rosana Silva Corpes Kyouk Isabel Portilho dos Santos Ivoneide Maria Menezes Barra Helder Kiyoshi Miyagawa Alberdan Silva Santos |
| author_facet | Alessandra Carla Guimarães Sobrinho Rosana Silva Corpes Kyouk Isabel Portilho dos Santos Ivoneide Maria Menezes Barra Helder Kiyoshi Miyagawa Alberdan Silva Santos |
| author_sort | Alessandra Carla Guimarães Sobrinho |
| collection | DOAJ |
| description | Hibiscus sabdariffa L. is a naturalized medicinal species in Brazil commonly called a “vinagreira” and is a member of the Malvaceae Juss. family, which has a rich potential of bioactive compounds presenting extracts with antioxidant, antibacterial, anti-inflammatory, hepatoprotective, antiviral, antidiabetic, and antiobesity, among others. The production of secondary metabolites of medicinal plants using biotechnological tools such as the culture of callus of plant tissues is increasingly being used to produce high-quality compounds under in vitro conditions. From this perspective, the objective of this work was to analyze the chemical compounds of the leaves and callus culture of H. sabdariffa using techniques of Gas Chromatography Coupled to the Mass Spectrum (GC-MS),. The analysis methodology used consisted of removal of liposoluble compounds, acid hydrolysis, and derivatization, all stages were submitted to ultrasonic-assisted agitation, using a reduced amount of biomass. Based on the results obtained in the study, a total of 38 metabolites identified by GC-MS analysis can be observed. Among the identified substances, protocatechuic acid (26A) stands out as the main constituent, with a relative abundance of 26.86% and 16.68% for leaves and callus of H. sabdariffa, respectively. The principal component analysis (PCA) allowed the discrimination of the chemical composition of each sample, being useful for the observation and detection of the compounds trends patterns. The analysis of the hierarchical group combined with the heat map represented the visual relationship between the samples of the data set indicating the values of higher and lower concentrations of chemical compounds respectively, confirming that protocatechuic acid is the most abundant, for the leaves and callus of H. sabdariffa, followed by eicosanoid and isocitric acid, produced only in callus. It was concluded that the GC-MS technique combined with chemometric tools, helped identify the diversity of the compounds present in the leaves and callus of H. sabdariffa and that callus culture enables the production of bioactive compounds continuously and uniformly in a controlled environment and free of contamination. |
| first_indexed | 2024-04-13T05:12:37Z |
| format | Article |
| id | doaj.art-2c28ff7063864de8ba81be6677782664 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-13T05:12:37Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-2c28ff7063864de8ba81be66777826642022-12-22T03:00:59ZengElsevierArabian Journal of Chemistry1878-53522022-09-01159104103Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L.Alessandra Carla Guimarães Sobrinho0Rosana Silva Corpes1Kyouk Isabel Portilho dos Santos2Ivoneide Maria Menezes Barra3Helder Kiyoshi Miyagawa4Alberdan Silva Santos5Federal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, BrazilFederal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, BrazilFederal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, BrazilFederal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, BrazilFederal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, BrazilCorresponding author at: Federal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, Brazil.; Federal University of Para. Laboratory of Systematic Investigation In Biotechnology and Molecular Biodiversity. CEP. 66075-110. R. Augusto Corrrea, 01. Guamá, Belém, Pará, BrazilHibiscus sabdariffa L. is a naturalized medicinal species in Brazil commonly called a “vinagreira” and is a member of the Malvaceae Juss. family, which has a rich potential of bioactive compounds presenting extracts with antioxidant, antibacterial, anti-inflammatory, hepatoprotective, antiviral, antidiabetic, and antiobesity, among others. The production of secondary metabolites of medicinal plants using biotechnological tools such as the culture of callus of plant tissues is increasingly being used to produce high-quality compounds under in vitro conditions. From this perspective, the objective of this work was to analyze the chemical compounds of the leaves and callus culture of H. sabdariffa using techniques of Gas Chromatography Coupled to the Mass Spectrum (GC-MS),. The analysis methodology used consisted of removal of liposoluble compounds, acid hydrolysis, and derivatization, all stages were submitted to ultrasonic-assisted agitation, using a reduced amount of biomass. Based on the results obtained in the study, a total of 38 metabolites identified by GC-MS analysis can be observed. Among the identified substances, protocatechuic acid (26A) stands out as the main constituent, with a relative abundance of 26.86% and 16.68% for leaves and callus of H. sabdariffa, respectively. The principal component analysis (PCA) allowed the discrimination of the chemical composition of each sample, being useful for the observation and detection of the compounds trends patterns. The analysis of the hierarchical group combined with the heat map represented the visual relationship between the samples of the data set indicating the values of higher and lower concentrations of chemical compounds respectively, confirming that protocatechuic acid is the most abundant, for the leaves and callus of H. sabdariffa, followed by eicosanoid and isocitric acid, produced only in callus. It was concluded that the GC-MS technique combined with chemometric tools, helped identify the diversity of the compounds present in the leaves and callus of H. sabdariffa and that callus culture enables the production of bioactive compounds continuously and uniformly in a controlled environment and free of contamination.http://www.sciencedirect.com/science/article/pii/S1878535222004191VinagreiraMedicinal compoundsBiotechnologyMetabolomicsHibiscus |
| spellingShingle | Alessandra Carla Guimarães Sobrinho Rosana Silva Corpes Kyouk Isabel Portilho dos Santos Ivoneide Maria Menezes Barra Helder Kiyoshi Miyagawa Alberdan Silva Santos Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L. Arabian Journal of Chemistry Vinagreira Medicinal compounds Biotechnology Metabolomics Hibiscus |
| title | Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L. |
| title_full | Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L. |
| title_fullStr | Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L. |
| title_full_unstemmed | Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L. |
| title_short | Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L. |
| title_sort | untargeted gc ms metabolomics applied to wild leaves and callus produced by plant tissue culture of hibiscus sabdariffa l |
| topic | Vinagreira Medicinal compounds Biotechnology Metabolomics Hibiscus |
| url | http://www.sciencedirect.com/science/article/pii/S1878535222004191 |
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