EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM
ABSTRACT Objectives: The purpose of this study was to explore the cytotoxic effects of five different universal bonding agents on mouse fibroblast cell lines (L929). Materials and Methods: Five different widely used universal adhesive systems were chosen that have different contents, pH levels, and...
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| Format: | Article |
| Language: | English |
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Cumhuriyet University
2020-12-01
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| Series: | Cumhuriyet Dental Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1282034 |
| _version_ | 1797703775358550016 |
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| author | Derya Surmelioglu Sevim Atılan Yavuz |
| author_facet | Derya Surmelioglu Sevim Atılan Yavuz |
| author_sort | Derya Surmelioglu |
| collection | DOAJ |
| description | ABSTRACT
Objectives: The purpose of this study was to explore the cytotoxic effects of five different universal bonding agents on mouse fibroblast cell lines (L929).
Materials and Methods: Five different widely used universal adhesive systems were chosen that have different contents, pH levels, and polymerization methods. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Germany, and ACEA Biosciences, USA) was used for cytotoxic evaluation of light-cured polymerized G-Premio Bond (GC Europe, Belgium), Prime&Bond Universal (Dentsply Sirona, USA), Universal Bond Quick (Kuraray, USA), Single Bond Universal (3M ESPE, USA) and self-cured polymerized Tokuyama Universal Bond (Tokuyama, USA) experimental groups. L929 were cultured in Dulbecco’s modified Eagle’s medium and supplemented with 10% fetal bovine serum and 1 % antibiotics. The assay was performed E-plate-16 and monitored every 15 min for 72 h. Statistical analysis was performed using ANOVA and Tukey’s posthoc tests.
Results: All tested universal adhesive systems showed a statistically significant difference in cytotoxicity values in different time periods (p<0.05). Among the groups compared, G-Premio Bond showed the least cytotoxic effect; and Tokuyama Universal Bond showed the most cytotoxic effect. Different times of all universal adhesive systems significantly increased the count of viable cells compared to the control group (p<0.05).
Conclusions: In dentistry, universal adhesive systems can be observed cytotoxic effects to live cells. The evaluation of cytotoxicity with xCELLigence device is a reliable method and should be supported by new studies on this subject. |
| first_indexed | 2024-03-12T05:09:27Z |
| format | Article |
| id | doaj.art-2c2b55a51d42465b9759907e6761e4c0 |
| institution | Directory Open Access Journal |
| issn | 1302-5805 2146-2852 |
| language | English |
| last_indexed | 2024-03-12T05:09:27Z |
| publishDate | 2020-12-01 |
| publisher | Cumhuriyet University |
| record_format | Article |
| series | Cumhuriyet Dental Journal |
| spelling | doaj.art-2c2b55a51d42465b9759907e6761e4c02023-09-03T08:41:51ZengCumhuriyet UniversityCumhuriyet Dental Journal1302-58052146-28522020-12-0123437138110.7126/cumudj.7926992057EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEMDerya Surmelioglu0Sevim Atılan Yavuz1GAZIANTEP UNIVERSITY, FACULTY OF DENTISTRYGAZIANTEP UNIVERSITY, FACULTY OF DENTISTRYABSTRACT Objectives: The purpose of this study was to explore the cytotoxic effects of five different universal bonding agents on mouse fibroblast cell lines (L929). Materials and Methods: Five different widely used universal adhesive systems were chosen that have different contents, pH levels, and polymerization methods. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Germany, and ACEA Biosciences, USA) was used for cytotoxic evaluation of light-cured polymerized G-Premio Bond (GC Europe, Belgium), Prime&Bond Universal (Dentsply Sirona, USA), Universal Bond Quick (Kuraray, USA), Single Bond Universal (3M ESPE, USA) and self-cured polymerized Tokuyama Universal Bond (Tokuyama, USA) experimental groups. L929 were cultured in Dulbecco’s modified Eagle’s medium and supplemented with 10% fetal bovine serum and 1 % antibiotics. The assay was performed E-plate-16 and monitored every 15 min for 72 h. Statistical analysis was performed using ANOVA and Tukey’s posthoc tests. Results: All tested universal adhesive systems showed a statistically significant difference in cytotoxicity values in different time periods (p<0.05). Among the groups compared, G-Premio Bond showed the least cytotoxic effect; and Tokuyama Universal Bond showed the most cytotoxic effect. Different times of all universal adhesive systems significantly increased the count of viable cells compared to the control group (p<0.05). Conclusions: In dentistry, universal adhesive systems can be observed cytotoxic effects to live cells. The evaluation of cytotoxicity with xCELLigence device is a reliable method and should be supported by new studies on this subject.https://dergipark.org.tr/tr/download/article-file/1282034xcelligencecytotoxicityuniversal adhesive systemsl929 |
| spellingShingle | Derya Surmelioglu Sevim Atılan Yavuz EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM Cumhuriyet Dental Journal xcelligence cytotoxicity universal adhesive systems l929 |
| title | EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM |
| title_full | EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM |
| title_fullStr | EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM |
| title_full_unstemmed | EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM |
| title_short | EVALUATION OF CYTOTOXICITY OF DIFFERENT UNIVERSAL BONDS USING THE XCELLIGENCE SYSTEM |
| title_sort | evaluation of cytotoxicity of different universal bonds using the xcelligence system |
| topic | xcelligence cytotoxicity universal adhesive systems l929 |
| url | https://dergipark.org.tr/tr/download/article-file/1282034 |
| work_keys_str_mv | AT deryasurmelioglu evaluationofcytotoxicityofdifferentuniversalbondsusingthexcelligencesystem AT sevimatılanyavuz evaluationofcytotoxicityofdifferentuniversalbondsusingthexcelligencesystem |