Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-11-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-021-00483-6 |
| _version_ | 1818651709435543552 |
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| author | Alemayehu Dugassa Tesfaye Alemu Yitbarek Woldehawariat |
| author_facet | Alemayehu Dugassa Tesfaye Alemu Yitbarek Woldehawariat |
| author_sort | Alemayehu Dugassa |
| collection | DOAJ |
| description | Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits. Results Pseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety. Conclusions The results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended. |
| first_indexed | 2024-12-17T02:10:25Z |
| format | Article |
| id | doaj.art-2c310c2daa434b1eba34643cbd5d95d6 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-17T02:10:25Z |
| publishDate | 2021-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-2c310c2daa434b1eba34643cbd5d95d62022-12-21T22:07:34ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422021-11-0131111310.1186/s41938-021-00483-6Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciaeAlemayehu Dugassa0Tesfaye Alemu1Yitbarek Woldehawariat2Department of Microbial, Cellular and Molecular Biology, College of Natural and Computational Sciences, Addis Ababa UniversityDepartment of Microbial, Cellular and Molecular Biology, College of Natural and Computational Sciences, Addis Ababa UniversityDepartment of Zoological Science, College of Natural and Computational Sciences, Addis Ababa UniversityAbstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits. Results Pseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety. Conclusions The results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended.https://doi.org/10.1186/s41938-021-00483-6BiocontrolCo-inoculationGall diseaseIn-vivoRhizobacteriaVicia faba |
| spellingShingle | Alemayehu Dugassa Tesfaye Alemu Yitbarek Woldehawariat Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae Egyptian Journal of Biological Pest Control Biocontrol Co-inoculation Gall disease In-vivo Rhizobacteria Vicia faba |
| title | Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae |
| title_full | Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae |
| title_fullStr | Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae |
| title_full_unstemmed | Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae |
| title_short | Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae |
| title_sort | screening of indigenous rhizobacteria as potential biological control against faba bean vicia faba l gall disease caused by olpidium viciae |
| topic | Biocontrol Co-inoculation Gall disease In-vivo Rhizobacteria Vicia faba |
| url | https://doi.org/10.1186/s41938-021-00483-6 |
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