Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay
The study aimed to assess the genotoxicity of different DDT contaminated soils (with different amendment types) in benzene extracts. A Salmonella/microsome assay without metabolic activation (-S9) was performed to evaluate the efficiency to initiate the mutations of remediated and non-remediated soi...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/19/bioconf_ifbioscfu2024_03016.pdf |
| _version_ | 1797213166696923136 |
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| author | Turganova Ronagul Koishekenova Gulshat Zaypanova Saule Delannoy Matthieu Jurjanz Stefan Bekmanov Bakhytzhan |
| author_facet | Turganova Ronagul Koishekenova Gulshat Zaypanova Saule Delannoy Matthieu Jurjanz Stefan Bekmanov Bakhytzhan |
| author_sort | Turganova Ronagul |
| collection | DOAJ |
| description | The study aimed to assess the genotoxicity of different DDT contaminated soils (with different amendment types) in benzene extracts. A Salmonella/microsome assay without metabolic activation (-S9) was performed to evaluate the efficiency to initiate the mutations of remediated and non-remediated soils. The number of revertants mutations in his+ in TA100 and TA98 strains were obtained, and compared to soils without DDT, and remediated DDT containing soils. The interpretation of the data suggests that, mutagenic activity was high for the non-remediated soils samples Kyzylkairat and Beskainar, and revealed in the scale as a weak mutagen. Soil samples treated with DARCO, ORBO, Sarg<150μm, and Sarg>150μm in Kyzylkairat and Beskainar exhibited a decreasing level of mutagenic activity, which is in line with the decreased environmental availability in these soil. Overall, the study indicates that strains TA100 and TA98 are valuable tools for rapidly assessing the potential mutagenicity of soil contaminated with OCPs. |
| first_indexed | 2024-04-24T10:53:58Z |
| format | Article |
| id | doaj.art-2c310d00d7f143c6abb91f4beacfc86f |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-04-24T10:53:58Z |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-2c310d00d7f143c6abb91f4beacfc86f2024-04-12T07:36:49ZengEDP SciencesBIO Web of Conferences2117-44582024-01-011000301610.1051/bioconf/202410003016bioconf_ifbioscfu2024_03016Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assayTurganova Ronagul0Koishekenova Gulshat1Zaypanova Saule2Delannoy Matthieu3Jurjanz Stefan4Bekmanov Bakhytzhan5Al-Farabi Kazakh National University, Faculty of Biology and BiotechnologyInstitute of Genetics and Physiology, Institute of Molecular GeneticsInstitute of Genetics and Physiology, Institute of Molecular GeneticsUniversité de Lorraine, INRAE, Laboratoire Animal et AgroécosystèmesUniversité de Lorraine, INRAE, Laboratoire Animal et AgroécosystèmesAl-Farabi Kazakh National University, Faculty of Biology and BiotechnologyThe study aimed to assess the genotoxicity of different DDT contaminated soils (with different amendment types) in benzene extracts. A Salmonella/microsome assay without metabolic activation (-S9) was performed to evaluate the efficiency to initiate the mutations of remediated and non-remediated soils. The number of revertants mutations in his+ in TA100 and TA98 strains were obtained, and compared to soils without DDT, and remediated DDT containing soils. The interpretation of the data suggests that, mutagenic activity was high for the non-remediated soils samples Kyzylkairat and Beskainar, and revealed in the scale as a weak mutagen. Soil samples treated with DARCO, ORBO, Sarg<150μm, and Sarg>150μm in Kyzylkairat and Beskainar exhibited a decreasing level of mutagenic activity, which is in line with the decreased environmental availability in these soil. Overall, the study indicates that strains TA100 and TA98 are valuable tools for rapidly assessing the potential mutagenicity of soil contaminated with OCPs.https://www.bio-conferences.org/articles/bioconf/pdf/2024/19/bioconf_ifbioscfu2024_03016.pdf |
| spellingShingle | Turganova Ronagul Koishekenova Gulshat Zaypanova Saule Delannoy Matthieu Jurjanz Stefan Bekmanov Bakhytzhan Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay BIO Web of Conferences |
| title | Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay |
| title_full | Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay |
| title_fullStr | Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay |
| title_full_unstemmed | Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay |
| title_short | Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay |
| title_sort | assessment of the genotoxic potential of soil associated ddts and its metabolites using the salmonella microsome assay |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/19/bioconf_ifbioscfu2024_03016.pdf |
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