Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples
The objective of this study was an improvement on oat identification procedure for laboratory applications, and the comparison of albumin-globulin and avenin protein patterns in five hulled and naked oat cultivars: Abel (CZ) and Izák (CZ) - naked oats, Auron (CZ), Edmund (D) and Expander (D) - hulle...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2003-11-01
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| Series: | Plant, Soil and Environment |
| Subjects: | |
| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200311-0002_suitability-of-oat-seed-storage-protein-markers-for-identification-of-cultivars-in-grain-and-mixed-flour-sample.php |
| _version_ | 1797898397107093504 |
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| author | V. Dvořáček V. Čurn J. Moudrý |
| author_facet | V. Dvořáček V. Čurn J. Moudrý |
| author_sort | V. Dvořáček |
| collection | DOAJ |
| description | The objective of this study was an improvement on oat identification procedure for laboratory applications, and the comparison of albumin-globulin and avenin protein patterns in five hulled and naked oat cultivars: Abel (CZ) and Izák (CZ) - naked oats, Auron (CZ), Edmund (D) and Expander (D) - hulled oats. The last object of this study was the authenticity verification of standardly prepared meal samples with various proportions of admixture. It was confirmed that avenins, characterised under SDS-PAGE conditions, are reliable implements for the identification of oat cultivars. It was found that oat grain contains, on the basis of Osborne fractionation, another significant protein fraction - glutelins. The question of the protein fraction analysis that was used for the admixture identification stays still open. In sufficiently different cultivars, the certainty of the admixture detection in meal samples may be high. Nevertheless, in other cases (higher cultivar similarity) it will be necessary to use some other, more sensitive techniques. |
| first_indexed | 2024-04-10T08:12:44Z |
| format | Article |
| id | doaj.art-2c3399dca0ca438ca47ddece7513a97a |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:12:44Z |
| publishDate | 2003-11-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-2c3399dca0ca438ca47ddece7513a97a2023-02-23T03:44:53ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682003-11-01491148649110.17221/4182-PSEpse-200311-0002Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samplesV. Dvořáček0V. Čurn1J. Moudrý2Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production, Prague-Ruzyně, Czech RepublicThe objective of this study was an improvement on oat identification procedure for laboratory applications, and the comparison of albumin-globulin and avenin protein patterns in five hulled and naked oat cultivars: Abel (CZ) and Izák (CZ) - naked oats, Auron (CZ), Edmund (D) and Expander (D) - hulled oats. The last object of this study was the authenticity verification of standardly prepared meal samples with various proportions of admixture. It was confirmed that avenins, characterised under SDS-PAGE conditions, are reliable implements for the identification of oat cultivars. It was found that oat grain contains, on the basis of Osborne fractionation, another significant protein fraction - glutelins. The question of the protein fraction analysis that was used for the admixture identification stays still open. In sufficiently different cultivars, the certainty of the admixture detection in meal samples may be high. Nevertheless, in other cases (higher cultivar similarity) it will be necessary to use some other, more sensitive techniques.https://pse.agriculturejournals.cz/artkey/pse-200311-0002_suitability-of-oat-seed-storage-protein-markers-for-identification-of-cultivars-in-grain-and-mixed-flour-sample.phpoatcultivar identificationprotein fractionelectrophoresispage |
| spellingShingle | V. Dvořáček V. Čurn J. Moudrý Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples Plant, Soil and Environment oat cultivar identification protein fraction electrophoresis page |
| title | Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples |
| title_full | Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples |
| title_fullStr | Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples |
| title_full_unstemmed | Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples |
| title_short | Suitability of oat-seed storage-protein markers for identification of cultivars in grain and mixed flour samples |
| title_sort | suitability of oat seed storage protein markers for identification of cultivars in grain and mixed flour samples |
| topic | oat cultivar identification protein fraction electrophoresis page |
| url | https://pse.agriculturejournals.cz/artkey/pse-200311-0002_suitability-of-oat-seed-storage-protein-markers-for-identification-of-cultivars-in-grain-and-mixed-flour-sample.php |
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