<i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA
Strain MS-P2<sup>T</sup> was isolated from microbial mats associated with Mushroom Spring, an alkaline siliceous hot spring in Yellowstone National Park, WY, USA. The isolate grows chemoheterotrophically by oxygen-dependent respiration, and light stimulates photoheterotrophic growth unde...
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MDPI AG
2021-12-01
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| author | Mohit Kumar Saini Shohei Yoshida Aswathy Sebastian Eri Hara Hideyuki Tamaki Nathan T. Soulier Istvan Albert Satoshi Hanada Marcus Tank Donald A. Bryant |
| author_facet | Mohit Kumar Saini Shohei Yoshida Aswathy Sebastian Eri Hara Hideyuki Tamaki Nathan T. Soulier Istvan Albert Satoshi Hanada Marcus Tank Donald A. Bryant |
| author_sort | Mohit Kumar Saini |
| collection | DOAJ |
| description | Strain MS-P2<sup>T</sup> was isolated from microbial mats associated with Mushroom Spring, an alkaline siliceous hot spring in Yellowstone National Park, WY, USA. The isolate grows chemoheterotrophically by oxygen-dependent respiration, and light stimulates photoheterotrophic growth under strictly oxic conditions. Strain MS-P2<sup>T</sup> synthesizes bacteriochlorophyll <i>a</i> and the carotenoid spirilloxanthin. However, photoautotrophic growth did not occur under oxic or anoxic conditions, suggesting that this strain should be classified as an aerobic anoxygenic phototrophic bacterium. Strain MS-P2<sup>T</sup> cells are motile, curved rods about 0.5 to 1.0 μm wide and 1.0 to 1.5 μm long. The optimum growth temperature is 45–50 °C, and the optimum pH for growth is circum-neutral (pH 7.0–7.5). Sequence analysis of the 16S rRNA gene revealed that strain MS-P2<sup>T</sup> is closely related to <i>Elioraea</i> species, members of the class <i>Alphaproteobacteria</i>, with a sequence identity of 96.58 to 98%. The genome of strain MS-P2<sup>T</sup> is a single circular DNA molecule of 3,367,643 bp with a mol% guanine-plus-cytosine content of 70.6%. Based on phylogenetic, physiological, biochemical, and genomic characteristics, we propose this bacteriochlorophyll <i>a</i>-containing isolate is a new species belonging to the genus <i>Elioraea</i>, with the suggested name <i>Elioraea</i><i>tepida</i>. The type-strain is strain MS-P2<sup>T</sup> (= JCM33060<sup>T</sup> = ATCC TSD-174<sup>T</sup>). |
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| spelling | doaj.art-2c3514b97ee849df8623fdeb7cd3065b2023-11-23T14:47:01ZengMDPI AGMicroorganisms2076-26072021-12-011018010.3390/microorganisms10010080<i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USAMohit Kumar Saini0Shohei Yoshida1Aswathy Sebastian2Eri Hara3Hideyuki Tamaki4Nathan T. Soulier5Istvan Albert6Satoshi Hanada7Marcus Tank8Donald A. Bryant9Department of Biological Sciences, Tokyo Metropolitan University, Tokyo 192-0397, JapanDepartment of Biological Sciences, Tokyo Metropolitan University, Tokyo 192-0397, JapanThe Huck Institutes for the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USABioproduction Research Institute—National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba 305-8566, JapanBioproduction Research Institute—National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba 305-8566, JapanDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USAThe Huck Institutes for the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USADepartment of Biological Sciences, Tokyo Metropolitan University, Tokyo 192-0397, JapanDepartment of Biological Sciences, Tokyo Metropolitan University, Tokyo 192-0397, JapanDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USAStrain MS-P2<sup>T</sup> was isolated from microbial mats associated with Mushroom Spring, an alkaline siliceous hot spring in Yellowstone National Park, WY, USA. The isolate grows chemoheterotrophically by oxygen-dependent respiration, and light stimulates photoheterotrophic growth under strictly oxic conditions. Strain MS-P2<sup>T</sup> synthesizes bacteriochlorophyll <i>a</i> and the carotenoid spirilloxanthin. However, photoautotrophic growth did not occur under oxic or anoxic conditions, suggesting that this strain should be classified as an aerobic anoxygenic phototrophic bacterium. Strain MS-P2<sup>T</sup> cells are motile, curved rods about 0.5 to 1.0 μm wide and 1.0 to 1.5 μm long. The optimum growth temperature is 45–50 °C, and the optimum pH for growth is circum-neutral (pH 7.0–7.5). Sequence analysis of the 16S rRNA gene revealed that strain MS-P2<sup>T</sup> is closely related to <i>Elioraea</i> species, members of the class <i>Alphaproteobacteria</i>, with a sequence identity of 96.58 to 98%. The genome of strain MS-P2<sup>T</sup> is a single circular DNA molecule of 3,367,643 bp with a mol% guanine-plus-cytosine content of 70.6%. Based on phylogenetic, physiological, biochemical, and genomic characteristics, we propose this bacteriochlorophyll <i>a</i>-containing isolate is a new species belonging to the genus <i>Elioraea</i>, with the suggested name <i>Elioraea</i><i>tepida</i>. The type-strain is strain MS-P2<sup>T</sup> (= JCM33060<sup>T</sup> = ATCC TSD-174<sup>T</sup>).https://www.mdpi.com/2076-2607/10/1/80aerobic anoxygenic phototrophic bacteriachlorophototrophthermophilehot springbacteriochlorophyll<i>Alphaproteobacteria</i> |
| spellingShingle | Mohit Kumar Saini Shohei Yoshida Aswathy Sebastian Eri Hara Hideyuki Tamaki Nathan T. Soulier Istvan Albert Satoshi Hanada Marcus Tank Donald A. Bryant <i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA Microorganisms aerobic anoxygenic phototrophic bacteria chlorophototroph thermophile hot spring bacteriochlorophyll <i>Alphaproteobacteria</i> |
| title | <i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA |
| title_full | <i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA |
| title_fullStr | <i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA |
| title_full_unstemmed | <i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA |
| title_short | <i>Elioraea tepida,</i> sp. nov., a Moderately Thermophilic Aerobic Anoxygenic Phototrophic Bacterium Isolated from the Mat Community of an Alkaline Siliceous Hot Spring in Yellowstone National Park, WY, USA |
| title_sort | i elioraea tepida i sp nov a moderately thermophilic aerobic anoxygenic phototrophic bacterium isolated from the mat community of an alkaline siliceous hot spring in yellowstone national park wy usa |
| topic | aerobic anoxygenic phototrophic bacteria chlorophototroph thermophile hot spring bacteriochlorophyll <i>Alphaproteobacteria</i> |
| url | https://www.mdpi.com/2076-2607/10/1/80 |
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