A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration
Recently, the term theragenerative has been proposed for biomaterials capable of inducing therapeutic approaches followed by repairing/regenerating the tissue/organ. This study is focused on the design of a new theragenerative nanocomposite composed of an amphiphilic non-ionic surfactant (Pluronic F...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-05-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24000288 |
| _version_ | 1827265737805266944 |
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| author | Ashkan Bigham Ines Fasolino Silvia Borsacchi Carmen Valente Lucia Calucci Gabriele Turacchio Marianna Pannico Manuel Serrano-Ruiz Luigi Ambrosio Maria Grazia Raucci |
| author_facet | Ashkan Bigham Ines Fasolino Silvia Borsacchi Carmen Valente Lucia Calucci Gabriele Turacchio Marianna Pannico Manuel Serrano-Ruiz Luigi Ambrosio Maria Grazia Raucci |
| author_sort | Ashkan Bigham |
| collection | DOAJ |
| description | Recently, the term theragenerative has been proposed for biomaterials capable of inducing therapeutic approaches followed by repairing/regenerating the tissue/organ. This study is focused on the design of a new theragenerative nanocomposite composed of an amphiphilic non-ionic surfactant (Pluronic F127), bioactive glass (BG), and black phosphorus (BP). The nanocomposite was prepared through a two-step synthetic strategy, including a microwave treatment that turned BP nanosheets (BPNS) into quantum dots (BPQDs) with 5 ± 2 nm dimensions in situ. The effects of surfactant and microwave treatment were assessed in vitro: the surfactant distributes the ions homogenously throughout the composite and the microwave treatment chemically stabilizes the composite. The presence of BP enhanced bioactivity and promoted calcium phosphate formation in simulated body fluid. The inherent anticancer activity of BP-containing nanocomposites was tested against osteosarcoma cells in vitro, finding that 150 μg mL−1 was the lowest concentration which prevented the proliferation of SAOS-2 cells, while the counterpart without BP did not affect the cell growth rate. Moreover, the apoptosis pathways were evaluated and a mechanism of action was proposed. NIR irradiation was applied to induce further proliferation suppression on SAOS-2 cells through hyperthermia. The inhibitory effects of bare BP nanomaterials and nanocomposites on the migration and invasion of bone cancer, breast cancer, and prostate cancer cells were assessed in vitro to determine the anticancer potential of nanomaterials against primary and secondary bone cancers. The regenerative behavior of the nanocomposites was tested with healthy osteoblasts and human mesenchymal stem cells; the BPQDs-incorporated nanocomposite significantly promoted the proliferation of osteoblast cells and induced the osteogenic differentiation of stem cells. This study introduces a new multifunctional theragenerative platform with promising potential for simultaneous bone cancer therapy and regeneration. |
| first_indexed | 2024-03-08T11:42:15Z |
| format | Article |
| id | doaj.art-2c35fc2c361c4285a1e1c38b7b45760a |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2025-03-22T04:06:43Z |
| publishDate | 2024-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-2c35fc2c361c4285a1e1c38b7b45760a2024-04-28T09:08:09ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-05-013599121A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regenerationAshkan Bigham0Ines Fasolino1Silvia Borsacchi2Carmen Valente3Lucia Calucci4Gabriele Turacchio5Marianna Pannico6Manuel Serrano-Ruiz7Luigi Ambrosio8Maria Grazia Raucci9Institute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Viale John Fitzgerald Kennedy 54, Mostra d’Oltremare Padiglione 20, 80125, Naples, Italy; Department of Chemical, Materials and Production Engineering, University of Naples Federico II, Piazzale V. Tecchio 80, 80125, Naples, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Viale John Fitzgerald Kennedy 54, Mostra d’Oltremare Padiglione 20, 80125, Naples, ItalyInstitute for the Chemistry of OrganoMetallic Compounds-ICCOM, Italian National Research Council-CNR, via G. Moruzzi 1, 56124, Pisa, Italy; Center for Instrument Sharing of the University of Pisa (CISUP), 56126, Pisa, ItalyInstitute of Experimental Endocrinology and Oncology “G. Salvatore” (IEOS), National Research Council (CNR), Via Pietro Castellino 111, 80131, Napoli, ItalyInstitute for the Chemistry of OrganoMetallic Compounds-ICCOM, Italian National Research Council-CNR, via G. Moruzzi 1, 56124, Pisa, Italy; Center for Instrument Sharing of the University of Pisa (CISUP), 56126, Pisa, ItalyInstitute of Experimental Endocrinology and Oncology “G. Salvatore” (IEOS), National Research Council (CNR), Via Pietro Castellino 111, 80131, Napoli, ItalyInstitute of Polymers, Composites, and Biomaterials, National Research Council of Italy (IPCB-CNR), Pozzuoli, ItalyInstitute for the Chemistry of OrganoMetallic Compounds-ICCOM, National Research Council-CNR, Sesto Fiorentino, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Viale John Fitzgerald Kennedy 54, Mostra d’Oltremare Padiglione 20, 80125, Naples, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), Viale John Fitzgerald Kennedy 54, Mostra d’Oltremare Padiglione 20, 80125, Naples, Italy; Corresponding author.Recently, the term theragenerative has been proposed for biomaterials capable of inducing therapeutic approaches followed by repairing/regenerating the tissue/organ. This study is focused on the design of a new theragenerative nanocomposite composed of an amphiphilic non-ionic surfactant (Pluronic F127), bioactive glass (BG), and black phosphorus (BP). The nanocomposite was prepared through a two-step synthetic strategy, including a microwave treatment that turned BP nanosheets (BPNS) into quantum dots (BPQDs) with 5 ± 2 nm dimensions in situ. The effects of surfactant and microwave treatment were assessed in vitro: the surfactant distributes the ions homogenously throughout the composite and the microwave treatment chemically stabilizes the composite. The presence of BP enhanced bioactivity and promoted calcium phosphate formation in simulated body fluid. The inherent anticancer activity of BP-containing nanocomposites was tested against osteosarcoma cells in vitro, finding that 150 μg mL−1 was the lowest concentration which prevented the proliferation of SAOS-2 cells, while the counterpart without BP did not affect the cell growth rate. Moreover, the apoptosis pathways were evaluated and a mechanism of action was proposed. NIR irradiation was applied to induce further proliferation suppression on SAOS-2 cells through hyperthermia. The inhibitory effects of bare BP nanomaterials and nanocomposites on the migration and invasion of bone cancer, breast cancer, and prostate cancer cells were assessed in vitro to determine the anticancer potential of nanomaterials against primary and secondary bone cancers. The regenerative behavior of the nanocomposites was tested with healthy osteoblasts and human mesenchymal stem cells; the BPQDs-incorporated nanocomposite significantly promoted the proliferation of osteoblast cells and induced the osteogenic differentiation of stem cells. This study introduces a new multifunctional theragenerative platform with promising potential for simultaneous bone cancer therapy and regeneration.http://www.sciencedirect.com/science/article/pii/S2452199X24000288Bioactive glassBlack phosphorusMicrowave treatmentBone cancer therapyTissue regeneration |
| spellingShingle | Ashkan Bigham Ines Fasolino Silvia Borsacchi Carmen Valente Lucia Calucci Gabriele Turacchio Marianna Pannico Manuel Serrano-Ruiz Luigi Ambrosio Maria Grazia Raucci A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration Bioactive Materials Bioactive glass Black phosphorus Microwave treatment Bone cancer therapy Tissue regeneration |
| title | A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration |
| title_full | A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration |
| title_fullStr | A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration |
| title_full_unstemmed | A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration |
| title_short | A theragenerative bio-nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration |
| title_sort | theragenerative bio nanocomposite consisting of black phosphorus quantum dots for bone cancer therapy and regeneration |
| topic | Bioactive glass Black phosphorus Microwave treatment Bone cancer therapy Tissue regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24000288 |
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