Development of a qPCR to diagnose the genus Eimeria in bovines
Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2020-09-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433 |
| _version_ | 1828055325934092288 |
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| author | Sérgio Tosi Cardim Stefani Lino Cardim Mércia de Seixas Alessandra Taroda Priscilla Gomes Carneiro Dauton Luiz Zulpo Luiz Daniel de Barros Guilherme Luis Bortolloti João Luis Garcia |
| author_facet | Sérgio Tosi Cardim Stefani Lino Cardim Mércia de Seixas Alessandra Taroda Priscilla Gomes Carneiro Dauton Luiz Zulpo Luiz Daniel de Barros Guilherme Luis Bortolloti João Luis Garcia |
| author_sort | Sérgio Tosi Cardim |
| collection | DOAJ |
| description |
Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry).
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| first_indexed | 2024-04-10T20:36:52Z |
| format | Article |
| id | doaj.art-2c37a635377749dd9ec5137b50c0f667 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:36:52Z |
| publishDate | 2020-09-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-2c37a635377749dd9ec5137b50c0f6672023-01-24T20:30:07ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592020-09-0141610.5433/1679-0359.2020v41n6p2695Development of a qPCR to diagnose the genus Eimeria in bovinesSérgio Tosi Cardim0Stefani Lino Cardim1Mércia de Seixas2Alessandra Taroda3Priscilla Gomes Carneiro4Dauton Luiz Zulpo5Luiz Daniel de Barros6Guilherme Luis Bortolloti7João Luis Garcia8Universidade Norte do ParanáUniversidade Estadual de LondrinaUniversidade Norte do ParanáUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaPontifícia Universidade Católica do ParanáUniversidade Estadual de LondrinaUniversidade Norte do ParanáUniversidade Estadual de Londrina Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry). https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433qPCREimeriacoccidiosis18S rRNAcattle |
| spellingShingle | Sérgio Tosi Cardim Stefani Lino Cardim Mércia de Seixas Alessandra Taroda Priscilla Gomes Carneiro Dauton Luiz Zulpo Luiz Daniel de Barros Guilherme Luis Bortolloti João Luis Garcia Development of a qPCR to diagnose the genus Eimeria in bovines Semina: Ciências Agrárias qPCR Eimeria coccidiosis 18S rRNA cattle |
| title | Development of a qPCR to diagnose the genus Eimeria in bovines |
| title_full | Development of a qPCR to diagnose the genus Eimeria in bovines |
| title_fullStr | Development of a qPCR to diagnose the genus Eimeria in bovines |
| title_full_unstemmed | Development of a qPCR to diagnose the genus Eimeria in bovines |
| title_short | Development of a qPCR to diagnose the genus Eimeria in bovines |
| title_sort | development of a qpcr to diagnose the genus eimeria in bovines |
| topic | qPCR Eimeria coccidiosis 18S rRNA cattle |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433 |
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