Effects of diazoxide in experimental acute necrotizing pancreatitis
OBJECTIVE: We aimed to assess the effects of diazoxide on the mortality, pancreatic injury, and inflammatory response in an experimental model of acute pancreatitis. METHODS: Male Wistar rats (200-400 g) were divided randomly into two groups. Fifteen minutes before surgery, animals received physiol...
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| Format: | Article |
| Language: | English |
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Elsevier España
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| Series: | Clinics |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000200125&lng=en&tlng=en |
| _version_ | 1811317116721168384 |
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| author | Roberta de Oliveira Andrade Tiago Kunitake Marcia Kiyomi Koike Marcel C.C. Machado Heraldo Possolo Souza |
| author_facet | Roberta de Oliveira Andrade Tiago Kunitake Marcia Kiyomi Koike Marcel C.C. Machado Heraldo Possolo Souza |
| author_sort | Roberta de Oliveira Andrade |
| collection | DOAJ |
| description | OBJECTIVE: We aimed to assess the effects of diazoxide on the mortality, pancreatic injury, and inflammatory response in an experimental model of acute pancreatitis. METHODS: Male Wistar rats (200-400 g) were divided randomly into two groups. Fifteen minutes before surgery, animals received physiological (0.9%) saline (3 mL/kg) (control group) or 45 mg/kg diazoxide (treatment group) via the intravenous route. Acute pancreatitis was induced by injection of 2.5% sodium taurocholate via the biliopancreatic duct. Mortality (n=38) was observed for 72 h and analyzed by the Mantel-Cox Log-rank test. To study pancreatic lesions and systemic inflammation, rats (10 from each group) were killed 3 h after acute pancreatitis induction; ascites volume was measured and blood as well as pancreases were collected. Pancreatic injury was assessed according to Schmidt’s scale. Cytokine expression in plasma was evaluated by the multiplex method. RESULTS: Mortality at 72 h was 33% in the control group and 60% in the treatment group (p=0.07). Ascites volumes and plasma levels of cytokines between groups were similar. No difference was observed in edema or infiltration of inflammatory cells in pancreatic tissues from either group. However, necrosis of acinar cells was lower in the treatment group compared to the control group (3.5 vs. 3.75, p=0.015). CONCLUSIONS: Treatment with diazoxide can reduce necrosis of acinar cells in an experimental model of acute pancreatitis, but does not affect the inflammatory response or mortality after 72 h. |
| first_indexed | 2024-04-13T12:01:24Z |
| format | Article |
| id | doaj.art-2c38448f7bfa4db582d9fb94732123ae |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-04-13T12:01:24Z |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-2c38448f7bfa4db582d9fb94732123ae2022-12-22T02:47:46ZengElsevier EspañaClinics1807-59321980-532272212512910.6061/clinics/2017(02)10S1807-59322017000200125Effects of diazoxide in experimental acute necrotizing pancreatitisRoberta de Oliveira AndradeTiago KunitakeMarcia Kiyomi KoikeMarcel C.C. MachadoHeraldo Possolo SouzaOBJECTIVE: We aimed to assess the effects of diazoxide on the mortality, pancreatic injury, and inflammatory response in an experimental model of acute pancreatitis. METHODS: Male Wistar rats (200-400 g) were divided randomly into two groups. Fifteen minutes before surgery, animals received physiological (0.9%) saline (3 mL/kg) (control group) or 45 mg/kg diazoxide (treatment group) via the intravenous route. Acute pancreatitis was induced by injection of 2.5% sodium taurocholate via the biliopancreatic duct. Mortality (n=38) was observed for 72 h and analyzed by the Mantel-Cox Log-rank test. To study pancreatic lesions and systemic inflammation, rats (10 from each group) were killed 3 h after acute pancreatitis induction; ascites volume was measured and blood as well as pancreases were collected. Pancreatic injury was assessed according to Schmidt’s scale. Cytokine expression in plasma was evaluated by the multiplex method. RESULTS: Mortality at 72 h was 33% in the control group and 60% in the treatment group (p=0.07). Ascites volumes and plasma levels of cytokines between groups were similar. No difference was observed in edema or infiltration of inflammatory cells in pancreatic tissues from either group. However, necrosis of acinar cells was lower in the treatment group compared to the control group (3.5 vs. 3.75, p=0.015). CONCLUSIONS: Treatment with diazoxide can reduce necrosis of acinar cells in an experimental model of acute pancreatitis, but does not affect the inflammatory response or mortality after 72 h.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000200125&lng=en&tlng=enpancreatitisdiazoxiderats |
| spellingShingle | Roberta de Oliveira Andrade Tiago Kunitake Marcia Kiyomi Koike Marcel C.C. Machado Heraldo Possolo Souza Effects of diazoxide in experimental acute necrotizing pancreatitis Clinics pancreatitis diazoxide rats |
| title | Effects of diazoxide in experimental acute necrotizing pancreatitis |
| title_full | Effects of diazoxide in experimental acute necrotizing pancreatitis |
| title_fullStr | Effects of diazoxide in experimental acute necrotizing pancreatitis |
| title_full_unstemmed | Effects of diazoxide in experimental acute necrotizing pancreatitis |
| title_short | Effects of diazoxide in experimental acute necrotizing pancreatitis |
| title_sort | effects of diazoxide in experimental acute necrotizing pancreatitis |
| topic | pancreatitis diazoxide rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000200125&lng=en&tlng=en |
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