Glycation damage to organelles and their DNA increases during maize seedling development
Abstract Shoot development in maize begins when meristematic, non-pigmented cells at leaf base stop dividing and proceeds toward the expanded green cells of the leaf blade. During this transition, promitochondria and proplastids develop into mature organelles and their DNA becomes fragmented. Change...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-06454-7 |
| _version_ | 1818298125235781632 |
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| author | Diwaker Tripathi Delene J. Oldenburg Arnold J. Bendich |
| author_facet | Diwaker Tripathi Delene J. Oldenburg Arnold J. Bendich |
| author_sort | Diwaker Tripathi |
| collection | DOAJ |
| description | Abstract Shoot development in maize begins when meristematic, non-pigmented cells at leaf base stop dividing and proceeds toward the expanded green cells of the leaf blade. During this transition, promitochondria and proplastids develop into mature organelles and their DNA becomes fragmented. Changes in glycation damage during organelle development were measured for protein and DNA, as well as the glycating agent methyl glyoxal and the glycation-defense protein DJ-1 (known as Park7 in humans). Maize seedlings were grown under normal, non-stressful conditions. Nonetheless, we found that glycation damage, as well as defenses against glycation, follow the same developmental pattern we found previously for reactive oxygen species (ROS): as damage increases, damage-defense measures decrease. In addition, light-grown leaves had more glycation and less DJ-1 compared to dark-grown leaves. The demise of maize organellar DNA during development may therefore be attributed to both oxidative and glycation damage that is not repaired. The coordination between oxidative and glycation damage, as well as damage-response from the nucleus is also discussed. |
| first_indexed | 2024-12-13T04:30:21Z |
| format | Article |
| id | doaj.art-2c3ce130359848789f8ee4bade975e0a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T04:30:21Z |
| publishDate | 2022-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2c3ce130359848789f8ee4bade975e0a2022-12-21T23:59:34ZengNature PortfolioScientific Reports2045-23222022-02-0112111310.1038/s41598-022-06454-7Glycation damage to organelles and their DNA increases during maize seedling developmentDiwaker Tripathi0Delene J. Oldenburg1Arnold J. Bendich2Department of Biology, University of WashingtonDepartment of Biology, University of WashingtonDepartment of Biology, University of WashingtonAbstract Shoot development in maize begins when meristematic, non-pigmented cells at leaf base stop dividing and proceeds toward the expanded green cells of the leaf blade. During this transition, promitochondria and proplastids develop into mature organelles and their DNA becomes fragmented. Changes in glycation damage during organelle development were measured for protein and DNA, as well as the glycating agent methyl glyoxal and the glycation-defense protein DJ-1 (known as Park7 in humans). Maize seedlings were grown under normal, non-stressful conditions. Nonetheless, we found that glycation damage, as well as defenses against glycation, follow the same developmental pattern we found previously for reactive oxygen species (ROS): as damage increases, damage-defense measures decrease. In addition, light-grown leaves had more glycation and less DJ-1 compared to dark-grown leaves. The demise of maize organellar DNA during development may therefore be attributed to both oxidative and glycation damage that is not repaired. The coordination between oxidative and glycation damage, as well as damage-response from the nucleus is also discussed.https://doi.org/10.1038/s41598-022-06454-7 |
| spellingShingle | Diwaker Tripathi Delene J. Oldenburg Arnold J. Bendich Glycation damage to organelles and their DNA increases during maize seedling development Scientific Reports |
| title | Glycation damage to organelles and their DNA increases during maize seedling development |
| title_full | Glycation damage to organelles and their DNA increases during maize seedling development |
| title_fullStr | Glycation damage to organelles and their DNA increases during maize seedling development |
| title_full_unstemmed | Glycation damage to organelles and their DNA increases during maize seedling development |
| title_short | Glycation damage to organelles and their DNA increases during maize seedling development |
| title_sort | glycation damage to organelles and their dna increases during maize seedling development |
| url | https://doi.org/10.1038/s41598-022-06454-7 |
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