Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i>
The fungal pathogen <i>Magnaporthe oryzae</i> secretes a large number of effector proteins to facilitate infection, most of which are not functionally characterized. We selected potential candidate effector genes from the genome of <i>M. oryzae</i>, field isolate P131, and cl...
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MDPI AG
2023-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/5/574 |
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| author | Di Liu Zhiqin Lun Ning Liu Guixin Yuan Xingbin Wang Shanshan Li You-Liang Peng Xunli Lu |
| author_facet | Di Liu Zhiqin Lun Ning Liu Guixin Yuan Xingbin Wang Shanshan Li You-Liang Peng Xunli Lu |
| author_sort | Di Liu |
| collection | DOAJ |
| description | The fungal pathogen <i>Magnaporthe oryzae</i> secretes a large number of effector proteins to facilitate infection, most of which are not functionally characterized. We selected potential candidate effector genes from the genome of <i>M. oryzae</i>, field isolate P131, and cloned 69 putative effector genes for functional screening. Utilizing a rice protoplast transient expression system, we identified that four candidate effector genes, <i>GAS1</i>, <i>BAS2</i>, <i>MoCEP1</i> and <i>MoCEP2</i> induced cell death in rice. In particular, <i>MoCEP2</i> also induced cell death in <i>Nicotiana benthamiana</i> leaves through Agrobacteria-mediated transient gene expression. We further identified that six candidate effector genes, <i>MoCEP3</i> to <i>MoCEP8</i>, suppress flg22-induced ROS burst in <i>N. benthamiana</i> leaves upon transient expression. These effector genes were highly expressed at a different stage after <i>M. oryzae</i> infection. We successfully knocked out five genes in <i>M. oryzae</i>, <i>MoCEP1</i>, <i>MoCEP2</i>, <i>MoCEP3</i>, <i>MoCEP5</i> and <i>MoCEP7</i>. The virulence tests suggested that the deletion mutants of <i>MoCEP2</i>, <i>MoCEP3</i> and <i>MoCEP5</i> showed reduced virulence on rice and barley plants. Therefore, those genes play an important role in pathogenicity. |
| first_indexed | 2024-03-11T03:35:12Z |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T03:35:12Z |
| publishDate | 2023-05-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-2c3daee19572443db67ccc64e684e9e52023-11-18T02:02:32ZengMDPI AGJournal of Fungi2309-608X2023-05-019557410.3390/jof9050574Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i>Di Liu0Zhiqin Lun1Ning Liu2Guixin Yuan3Xingbin Wang4Shanshan Li5You-Liang Peng6Xunli Lu7MOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaMOA Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, ChinaThe fungal pathogen <i>Magnaporthe oryzae</i> secretes a large number of effector proteins to facilitate infection, most of which are not functionally characterized. We selected potential candidate effector genes from the genome of <i>M. oryzae</i>, field isolate P131, and cloned 69 putative effector genes for functional screening. Utilizing a rice protoplast transient expression system, we identified that four candidate effector genes, <i>GAS1</i>, <i>BAS2</i>, <i>MoCEP1</i> and <i>MoCEP2</i> induced cell death in rice. In particular, <i>MoCEP2</i> also induced cell death in <i>Nicotiana benthamiana</i> leaves through Agrobacteria-mediated transient gene expression. We further identified that six candidate effector genes, <i>MoCEP3</i> to <i>MoCEP8</i>, suppress flg22-induced ROS burst in <i>N. benthamiana</i> leaves upon transient expression. These effector genes were highly expressed at a different stage after <i>M. oryzae</i> infection. We successfully knocked out five genes in <i>M. oryzae</i>, <i>MoCEP1</i>, <i>MoCEP2</i>, <i>MoCEP3</i>, <i>MoCEP5</i> and <i>MoCEP7</i>. The virulence tests suggested that the deletion mutants of <i>MoCEP2</i>, <i>MoCEP3</i> and <i>MoCEP5</i> showed reduced virulence on rice and barley plants. Therefore, those genes play an important role in pathogenicity.https://www.mdpi.com/2309-608X/9/5/574<i>Magnaporthe oryzae</i>secreted effectorcell deathROSpathogenicity |
| spellingShingle | Di Liu Zhiqin Lun Ning Liu Guixin Yuan Xingbin Wang Shanshan Li You-Liang Peng Xunli Lu Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i> Journal of Fungi <i>Magnaporthe oryzae</i> secreted effector cell death ROS pathogenicity |
| title | Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i> |
| title_full | Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i> |
| title_fullStr | Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i> |
| title_full_unstemmed | Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i> |
| title_short | Identification and Characterization of Novel Candidate Effector Proteins from <i>Magnaporthe oryzae</i> |
| title_sort | identification and characterization of novel candidate effector proteins from i magnaporthe oryzae i |
| topic | <i>Magnaporthe oryzae</i> secreted effector cell death ROS pathogenicity |
| url | https://www.mdpi.com/2309-608X/9/5/574 |
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