Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis
Dentures and epitheses are mostly made from poly(methyl methacrylate) (PMMA), which does not show antimicrobial properties. They present reservoirs of microorganisms grown in biofilms. The aim of this study is to prepare a PMMA enriched with gold nanoparticles (AuNPs)-PMMA/AuNPs and the examination...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/7/1513 |
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| author | Vera Ivanovic Danica Popovic Sanja Petrovic Rebeka Rudolf Peter Majerič Milos Lazarevic Igor Djordjevic Vojkan Lazic Milena Radunovic |
| author_facet | Vera Ivanovic Danica Popovic Sanja Petrovic Rebeka Rudolf Peter Majerič Milos Lazarevic Igor Djordjevic Vojkan Lazic Milena Radunovic |
| author_sort | Vera Ivanovic |
| collection | DOAJ |
| description | Dentures and epitheses are mostly made from poly(methyl methacrylate) (PMMA), which does not show antimicrobial properties. They present reservoirs of microorganisms grown in biofilms. The aim of this study is to prepare a PMMA enriched with gold nanoparticles (AuNPs)-PMMA/AuNPs and the examination of its physical, mechanical and antimicrobial properties. The AuNPS were synthetized from HAuCl<sub>4</sub> using the ultrasonic spray pyrolysis method with lyophilization. The PMMA/AuNP samples were compared to PMMA samples. Density was measured by pycnometer. Microhardness was evaluated using the Vickers hardness test. Monomicrobial biofilm formation (<i>Streptococcus mitis</i>, <i>Candida albicans</i>, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>) was measured by colony-forming units (CFUs) and MTT test and visualized by SEM. AuNP release was measured indirectly (the CFUs of the medium around the sample). The density and microhardness of the PMMA/AuNPs were similar to those of the PMMA. CFU and MTT values for the biofilms formed on the PMMA for each of the tested species were higher than those of the biofilms formed on the PMMA/AuNPs. The CFUs of the medium around the sample were similar for both materials. PMMA/AuNPs showed a significant reduction in the monomicrobial biofilms of all tested species. AuNPs are not released from PMMA/AuNPs. Density, indirect measurement of residual monomer and dentures weight were similar between PMMA and PMMA/AuNPs. Microhardness, as a measure of the wear resistance, was also similar between tested discs. |
| first_indexed | 2024-03-09T06:03:04Z |
| format | Article |
| id | doaj.art-2c3dcce0fa3844668b997a4c797a807f |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T06:03:04Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2c3dcce0fa3844668b997a4c797a807f2023-12-03T12:06:34ZengMDPI AGPharmaceutics1999-49232022-07-01147151310.3390/pharmaceutics14071513Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and EpithesisVera Ivanovic0Danica Popovic1Sanja Petrovic2Rebeka Rudolf3Peter Majerič4Milos Lazarevic5Igor Djordjevic6Vojkan Lazic7Milena Radunovic8Laboratory for Microbiology, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaClinic for Prosthodontics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Microbiology, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Mechanical Engineering, University of Maribor, 2000 Maribor, SloveniaFaculty of Mechanical Engineering, University of Maribor, 2000 Maribor, SloveniaDepartment of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaClinic for Prosthodontics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaClinic for Prosthodontics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaLaboratory for Microbiology, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaDentures and epitheses are mostly made from poly(methyl methacrylate) (PMMA), which does not show antimicrobial properties. They present reservoirs of microorganisms grown in biofilms. The aim of this study is to prepare a PMMA enriched with gold nanoparticles (AuNPs)-PMMA/AuNPs and the examination of its physical, mechanical and antimicrobial properties. The AuNPS were synthetized from HAuCl<sub>4</sub> using the ultrasonic spray pyrolysis method with lyophilization. The PMMA/AuNP samples were compared to PMMA samples. Density was measured by pycnometer. Microhardness was evaluated using the Vickers hardness test. Monomicrobial biofilm formation (<i>Streptococcus mitis</i>, <i>Candida albicans</i>, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>) was measured by colony-forming units (CFUs) and MTT test and visualized by SEM. AuNP release was measured indirectly (the CFUs of the medium around the sample). The density and microhardness of the PMMA/AuNPs were similar to those of the PMMA. CFU and MTT values for the biofilms formed on the PMMA for each of the tested species were higher than those of the biofilms formed on the PMMA/AuNPs. The CFUs of the medium around the sample were similar for both materials. PMMA/AuNPs showed a significant reduction in the monomicrobial biofilms of all tested species. AuNPs are not released from PMMA/AuNPs. Density, indirect measurement of residual monomer and dentures weight were similar between PMMA and PMMA/AuNPs. Microhardness, as a measure of the wear resistance, was also similar between tested discs.https://www.mdpi.com/1999-4923/14/7/1513PMMAgold nanoparticles (AuNPs)biofilmantibiofilm effect |
| spellingShingle | Vera Ivanovic Danica Popovic Sanja Petrovic Rebeka Rudolf Peter Majerič Milos Lazarevic Igor Djordjevic Vojkan Lazic Milena Radunovic Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis Pharmaceutics PMMA gold nanoparticles (AuNPs) biofilm antibiofilm effect |
| title | Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis |
| title_full | Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis |
| title_fullStr | Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis |
| title_full_unstemmed | Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis |
| title_short | Unraveling the Antibiofilm Activity of a New Nanogold Resin for Dentures and Epithesis |
| title_sort | unraveling the antibiofilm activity of a new nanogold resin for dentures and epithesis |
| topic | PMMA gold nanoparticles (AuNPs) biofilm antibiofilm effect |
| url | https://www.mdpi.com/1999-4923/14/7/1513 |
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