Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China
Abstract Background Ganzi Prefecture in Western China is situated geographically at the transition regions between Tibetan Plateau and Sichuan Basin in a highly tectonically active boundary area between the India and Eurasia plates. The region hosts various hot springs that span a wide range of temp...
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-018-1271-z |
| _version_ | 1831701136090857472 |
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| author | Jie Tang Yuanmei Liang Dong Jiang Liheng Li Yifan Luo Md. Mahfuzur R. Shah Maurycy Daroch |
| author_facet | Jie Tang Yuanmei Liang Dong Jiang Liheng Li Yifan Luo Md. Mahfuzur R. Shah Maurycy Daroch |
| author_sort | Jie Tang |
| collection | DOAJ |
| description | Abstract Background Ganzi Prefecture in Western China is situated geographically at the transition regions between Tibetan Plateau and Sichuan Basin in a highly tectonically active boundary area between the India and Eurasia plates. The region hosts various hot springs that span a wide range of temperature from 30 to 98 °C and are located at high altitude (up to 4200 m above sea level) in the region of large geothermal anomalies and active Xianshuihe slip-fault that has been active since Holocene. The site represents a biodiversity reservoir for thermophiles, yet their diversity and relationship to geochemical parameters are largely unknown. In the present work, bacterial diversity and community structure in 14 hot springs of Ganzi were investigated using Illumina MiSeq sequencing. Results Bacterial community compositions were evidently distinct among the 14 hot springs, and the bacterial communities in hot springs were majorly abundant in phyla Aquificae, Cyanobacteria and Proteobacteria. Both clustering and PCoA analysis suggested the existence of four bacterial community patterns in these hot springs. Temperature contributed to shaping bacterial community structure of hot springs as revealed by correlation analysis. Abundant unassigned-genus sequences detected in this study strongly implied the presence of novel genera or genetic resources in these hot springs. Conclusion The diversity of hot springs of Ganzi prefecture in Western Sichuan, China is evidently shaped by temperature. Interestingly disproportionally abundant unassigned-genus sequences detected in this study show indicate potential of novel genera or phylotypes. We hypothesize that frequent earthquakes and rapidly changing environment might have contributed to evolution of these potentially new lineages. Overall, this study provided first insight into the bacterial diversity of hot springs located in Western Sichuan, China and its comparison with other similar communities worldwide. |
| first_indexed | 2024-12-20T14:54:57Z |
| format | Article |
| id | doaj.art-2c3f53b8704e4da4abc9db6369e66080 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-20T14:54:57Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-2c3f53b8704e4da4abc9db6369e660802022-12-21T19:36:52ZengBMCBMC Microbiology1471-21802018-10-0118111410.1186/s12866-018-1271-zTemperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, ChinaJie Tang0Yuanmei Liang1Dong Jiang2Liheng Li3Yifan Luo4Md. Mahfuzur R. Shah5Maurycy Daroch6School of Pharmacy and Bioengineering, Chengdu UniversitySchool of Environment and Energy, Peking University Shenzhen Graduate SchoolSchool of Environment and Energy, Peking University Shenzhen Graduate SchoolSchool of Environment and Energy, Peking University Shenzhen Graduate SchoolSchool of Environment and Energy, Peking University Shenzhen Graduate SchoolSchool of Environment and Energy, Peking University Shenzhen Graduate SchoolSchool of Environment and Energy, Peking University Shenzhen Graduate SchoolAbstract Background Ganzi Prefecture in Western China is situated geographically at the transition regions between Tibetan Plateau and Sichuan Basin in a highly tectonically active boundary area between the India and Eurasia plates. The region hosts various hot springs that span a wide range of temperature from 30 to 98 °C and are located at high altitude (up to 4200 m above sea level) in the region of large geothermal anomalies and active Xianshuihe slip-fault that has been active since Holocene. The site represents a biodiversity reservoir for thermophiles, yet their diversity and relationship to geochemical parameters are largely unknown. In the present work, bacterial diversity and community structure in 14 hot springs of Ganzi were investigated using Illumina MiSeq sequencing. Results Bacterial community compositions were evidently distinct among the 14 hot springs, and the bacterial communities in hot springs were majorly abundant in phyla Aquificae, Cyanobacteria and Proteobacteria. Both clustering and PCoA analysis suggested the existence of four bacterial community patterns in these hot springs. Temperature contributed to shaping bacterial community structure of hot springs as revealed by correlation analysis. Abundant unassigned-genus sequences detected in this study strongly implied the presence of novel genera or genetic resources in these hot springs. Conclusion The diversity of hot springs of Ganzi prefecture in Western Sichuan, China is evidently shaped by temperature. Interestingly disproportionally abundant unassigned-genus sequences detected in this study show indicate potential of novel genera or phylotypes. We hypothesize that frequent earthquakes and rapidly changing environment might have contributed to evolution of these potentially new lineages. Overall, this study provided first insight into the bacterial diversity of hot springs located in Western Sichuan, China and its comparison with other similar communities worldwide.http://link.springer.com/article/10.1186/s12866-018-1271-zBiodiversityHot springsThermophileMiSeq platform16S rRNAGanzi |
| spellingShingle | Jie Tang Yuanmei Liang Dong Jiang Liheng Li Yifan Luo Md. Mahfuzur R. Shah Maurycy Daroch Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China BMC Microbiology Biodiversity Hot springs Thermophile MiSeq platform 16S rRNA Ganzi |
| title | Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China |
| title_full | Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China |
| title_fullStr | Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China |
| title_full_unstemmed | Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China |
| title_short | Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan, China |
| title_sort | temperature controlled thermophilic bacterial communities in hot springs of western sichuan china |
| topic | Biodiversity Hot springs Thermophile MiSeq platform 16S rRNA Ganzi |
| url | http://link.springer.com/article/10.1186/s12866-018-1271-z |
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