Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia
A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80 °C–91 °C and pH 7–8. We used a simple method for culturing the microbes, by enriching the spring water with nut...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2015-07-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S197830191500008X |
| _version_ | 1817997086078009344 |
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| author | Heni Yohandini Julinar Muharni |
| author_facet | Heni Yohandini Julinar Muharni |
| author_sort | Heni Yohandini |
| collection | DOAJ |
| description | A community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80 °C–91 °C and pH 7–8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank. |
| first_indexed | 2024-04-14T02:33:03Z |
| format | Article |
| id | doaj.art-2c448faf0121431a92fcb99f8a483ca0 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-04-14T02:33:03Z |
| publishDate | 2015-07-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-2c448faf0121431a92fcb99f8a483ca02022-12-22T02:17:37ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192015-07-0122314314810.1016/j.hjb.2015.10.006Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, IndonesiaHeni Yohandini0Julinar1Muharni2Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662, IndonesiaDepartment of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662, IndonesiaDepartment of Biology, Faculty of Mathematic and Natural Sciences, Universitas Sriwijaya, Ogan Ilir 30662, IndonesiaA community of thermophiles within Tanjung Sakti Hot Spring (South Sumatera) have been cultivated and identified based on 16S ribosomal RNA gene sequence. The hot spring has temperature 80 °C–91 °C and pH 7–8. We used a simple method for culturing the microbes, by enriching the spring water with nutrient broth media. Phylogenetic analysis showed that the method could recover microbes, which clustered within four distinct taxonomic groups: Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. These microbes closely related to Anoxybacillus rupiensis, Anoxybacillus flavithermus, Geobacillus pallidus, Brevibacillus thermoruber, Bacillus licheniformis, and Bacillus thermoamylovorans. The 16S ribosomal RNA gene sequence of one isolate only had 96% similarity with Brevibacillus sequence in GenBank.http://www.sciencedirect.com/science/article/pii/S197830191500008X16S rRNAphylogenetic analysisTanjung Sakti Hot Springthermophile |
| spellingShingle | Heni Yohandini Julinar Muharni Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia Hayati Journal of Biosciences 16S rRNA phylogenetic analysis Tanjung Sakti Hot Spring thermophile |
| title | Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia |
| title_full | Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia |
| title_fullStr | Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia |
| title_full_unstemmed | Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia |
| title_short | Isolation and Phylogenetic Analysis of Thermophile Community Within Tanjung Sakti Hot Spring, South Sumatera, Indonesia |
| title_sort | isolation and phylogenetic analysis of thermophile community within tanjung sakti hot spring south sumatera indonesia |
| topic | 16S rRNA phylogenetic analysis Tanjung Sakti Hot Spring thermophile |
| url | http://www.sciencedirect.com/science/article/pii/S197830191500008X |
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