Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains widely pandemic around the world. Animal models that are sensitive to the virus are therefore urgently needed to evaluate potential vaccines and antiviral agents; however, SARS-CoV-2...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/11/7/1133 |
| _version_ | 1797587285201387520 |
|---|---|
| author | Xi Wu Nana Fang Ziteng Liang Jianhui Nie Sen Lang Changfa Fan Chunnan Liang Weijin Huang Youchun Wang |
| author_facet | Xi Wu Nana Fang Ziteng Liang Jianhui Nie Sen Lang Changfa Fan Chunnan Liang Weijin Huang Youchun Wang |
| author_sort | Xi Wu |
| collection | DOAJ |
| description | Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains widely pandemic around the world. Animal models that are sensitive to the virus are therefore urgently needed to evaluate potential vaccines and antiviral agents; however, SARS-CoV-2 requires biosafety level 3 containment. To overcome this, we developed an animal model using the intranasal administration of SARS-CoV-2 pseudovirus. As the pseudovirus contains the firefly luciferase reporter gene, infected tissues and the viral load could be monitored by in vivo bioluminescent imaging. We used the model to evaluate the protective efficacy of monoclonal antibodies and the tissue tropism of different variants. The model may also be a useful tool for the safe and convenient preliminary evaluation of the protective efficacy of vaccine candidates against SARS-CoV-2, as well as the treatment efficacy of anti-viral drugs. |
| first_indexed | 2024-03-11T00:35:00Z |
| format | Article |
| id | doaj.art-2c458ee476c244d288c6a8f94a8ad574 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T00:35:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-2c458ee476c244d288c6a8f94a8ad5742023-11-18T21:40:06ZengMDPI AGVaccines2076-393X2023-06-01117113310.3390/vaccines11071133Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus SystemXi Wu0Nana Fang1Ziteng Liang2Jianhui Nie3Sen Lang4Changfa Fan5Chunnan Liang6Weijin Huang7Youchun Wang8Division of HIV/AIDS and Sex-Transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaNational Vaccine and Serum Institute, Beijing 101111, ChinaDivision of HIV/AIDS and Sex-Transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaDivision of HIV/AIDS and Sex-Transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaNational Rodent Laboratory Animal Resources Center, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaNational Rodent Laboratory Animal Resources Center, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaNational Rodent Laboratory Animal Resources Center, Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaDivision of HIV/AIDS and Sex-Transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, ChinaChinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100006, ChinaCoronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains widely pandemic around the world. Animal models that are sensitive to the virus are therefore urgently needed to evaluate potential vaccines and antiviral agents; however, SARS-CoV-2 requires biosafety level 3 containment. To overcome this, we developed an animal model using the intranasal administration of SARS-CoV-2 pseudovirus. As the pseudovirus contains the firefly luciferase reporter gene, infected tissues and the viral load could be monitored by in vivo bioluminescent imaging. We used the model to evaluate the protective efficacy of monoclonal antibodies and the tissue tropism of different variants. The model may also be a useful tool for the safe and convenient preliminary evaluation of the protective efficacy of vaccine candidates against SARS-CoV-2, as well as the treatment efficacy of anti-viral drugs.https://www.mdpi.com/2076-393X/11/7/1133SARS-CoV-2 pseudovirusmouse modelin vivo bioluminescent |
| spellingShingle | Xi Wu Nana Fang Ziteng Liang Jianhui Nie Sen Lang Changfa Fan Chunnan Liang Weijin Huang Youchun Wang Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System Vaccines SARS-CoV-2 pseudovirus mouse model in vivo bioluminescent |
| title | Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System |
| title_full | Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System |
| title_fullStr | Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System |
| title_full_unstemmed | Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System |
| title_short | Development of a Bioluminescent Imaging Mouse Model for SARS-CoV-2 Infection Based on a Pseudovirus System |
| title_sort | development of a bioluminescent imaging mouse model for sars cov 2 infection based on a pseudovirus system |
| topic | SARS-CoV-2 pseudovirus mouse model in vivo bioluminescent |
| url | https://www.mdpi.com/2076-393X/11/7/1133 |
| work_keys_str_mv | AT xiwu developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT nanafang developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT zitengliang developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT jianhuinie developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT senlang developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT changfafan developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT chunnanliang developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT weijinhuang developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem AT youchunwang developmentofabioluminescentimagingmousemodelforsarscov2infectionbasedonapseudovirussystem |