Tetramer-aided sorting and single-cell RNA sequencing facilitate transcriptional profiling of antigen-specific CD8+ T cells

Tetramer-aided sorting and single-cell RNA sequencing facilitate transcriptional profiling of antigen-specific CD8+ T cells

Background: Recent advances in single-cell technologies and an improved understanding of tumor antigens have empowered researchers to investigate tumor antigen-specific CD8+ T cells at the single-cell level. Peptide-MHC I tetramers are often utilized to enrich antigen-specific CD8+ T cells, which ho...

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Bibliographic Details
Main Authors: Kamalakannan Rajasekaran, Xiangnan Guan, Alireza Tafazzol, Habib Hamidi, Martine Darwish, Mahesh Yadav
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Translational Oncology
Subjects:
CMV pp65-specific CD8+ T cells
pMHC Tetramers
FACS sorting
Single-cell RNA sequencing
TCR analysis
Antigen-specific response gene signature
Online Access:http://www.sciencedirect.com/science/article/pii/S1936523322002182

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