Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies
Biopolymer-based metal nanocomposites are gaining importance due to their safety, stability, and ease of synthesis. Chitosan and silver-based nanoparticles have been found to be effective as anti-fungal agents. In this study, we tested the fungicidal effect of green synthesized chitosan silver nanoc...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Plant Nano Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773111123000189 |
| _version_ | 1827374856821276672 |
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| author | Shivakumar Gowda Subbaraman Sriram |
| author_facet | Shivakumar Gowda Subbaraman Sriram |
| author_sort | Shivakumar Gowda |
| collection | DOAJ |
| description | Biopolymer-based metal nanocomposites are gaining importance due to their safety, stability, and ease of synthesis. Chitosan and silver-based nanoparticles have been found to be effective as anti-fungal agents. In this study, we tested the fungicidal effect of green synthesized chitosan silver nanocomposites (CS-AgNPs) against the chilli anthracnose pathogen Colletotrichum truncatum (syn. C. capsici). The nanoparticles were synthesized at 90 ± 1 °C under alkaline condition. Chitosan was used as a reducing and stabilizing agent in the synthesis. The formation of nanoparticles (NPs) was indicated by a change in the colour of the solution to yellow. UV-Visible spectroscopy of the synthesized nanoparticles showed a surface plasmon resonance peak between 406 and 420 nm. The synthesized silver nanoparticles were small, and the average particle size distribution was 4 nm, as characterized by TEM and FESEM. The Zeta potential measurement of the synthesized nanoparticles ranged from + 41.9 mV and + 50.5 mV. The nanoparticles were further characterized by XRD and FTIR analysis. The nanocomposites showed antifungal activity against C. truncatum in an in vitro conidial germination assay even at a concentration of 0.0005%. In vivo assay using detached chilli fruit (Capsicum annuum) showed that the nanocomposites significantly inhibited anthracnose in both preventive and curative measures. Even when applied at a concentration of 0.0625%, the nanocomposites exhibited growth-promoting activity with chilli seeds. Thus, the synthesized nanocomposites have a dual benefit of growth promotion as well as an effective antifungal agent in preventing postharvest anthracnose disease in chilli. |
| first_indexed | 2024-03-08T11:41:06Z |
| format | Article |
| id | doaj.art-2c47afcde8df443fbff1d17d8f6c4bff |
| institution | Directory Open Access Journal |
| issn | 2773-1111 |
| language | English |
| last_indexed | 2024-03-08T11:41:06Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Nano Biology |
| spelling | doaj.art-2c47afcde8df443fbff1d17d8f6c4bff2024-01-25T05:24:46ZengElsevierPlant Nano Biology2773-11112023-08-015100041Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chilliesShivakumar Gowda0Subbaraman Sriram1Division of Crop protection, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore 560089, India; Department of Biochemistry, School of Sciences, #34, 1st Cross, J C Road, Bangalore 560027, IndiaDivision of Crop protection, ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bangalore 560089, India; Corresponding author.Biopolymer-based metal nanocomposites are gaining importance due to their safety, stability, and ease of synthesis. Chitosan and silver-based nanoparticles have been found to be effective as anti-fungal agents. In this study, we tested the fungicidal effect of green synthesized chitosan silver nanocomposites (CS-AgNPs) against the chilli anthracnose pathogen Colletotrichum truncatum (syn. C. capsici). The nanoparticles were synthesized at 90 ± 1 °C under alkaline condition. Chitosan was used as a reducing and stabilizing agent in the synthesis. The formation of nanoparticles (NPs) was indicated by a change in the colour of the solution to yellow. UV-Visible spectroscopy of the synthesized nanoparticles showed a surface plasmon resonance peak between 406 and 420 nm. The synthesized silver nanoparticles were small, and the average particle size distribution was 4 nm, as characterized by TEM and FESEM. The Zeta potential measurement of the synthesized nanoparticles ranged from + 41.9 mV and + 50.5 mV. The nanoparticles were further characterized by XRD and FTIR analysis. The nanocomposites showed antifungal activity against C. truncatum in an in vitro conidial germination assay even at a concentration of 0.0005%. In vivo assay using detached chilli fruit (Capsicum annuum) showed that the nanocomposites significantly inhibited anthracnose in both preventive and curative measures. Even when applied at a concentration of 0.0625%, the nanocomposites exhibited growth-promoting activity with chilli seeds. Thus, the synthesized nanocomposites have a dual benefit of growth promotion as well as an effective antifungal agent in preventing postharvest anthracnose disease in chilli.http://www.sciencedirect.com/science/article/pii/S2773111123000189AnthracnoseAntifungal activityChilliChitosanColletotrichum truncatumPostharvest |
| spellingShingle | Shivakumar Gowda Subbaraman Sriram Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies Plant Nano Biology Anthracnose Antifungal activity Chilli Chitosan Colletotrichum truncatum Postharvest |
| title | Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies |
| title_full | Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies |
| title_fullStr | Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies |
| title_full_unstemmed | Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies |
| title_short | Green synthesis of chitosan silver nanocomposites and their antifungal activity against Colletotrichum truncatum causing anthracnose in chillies |
| title_sort | green synthesis of chitosan silver nanocomposites and their antifungal activity against colletotrichum truncatum causing anthracnose in chillies |
| topic | Anthracnose Antifungal activity Chilli Chitosan Colletotrichum truncatum Postharvest |
| url | http://www.sciencedirect.com/science/article/pii/S2773111123000189 |
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