In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications
Abstract Background In this paper we aimed to investigate the neovascularization and biodegradation of the silk fibroin in vivo using multiple modes ultrasound, including two-dimensional, three-dimensional and contrast-enhanced ultrasound by quantifying the echo intensity, volume and contrast enhanc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BioMedical Engineering OnLine |
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| Online Access: | http://link.springer.com/article/10.1186/s12938-018-0478-4 |
| _version_ | 1819060111229845504 |
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| author | Shouqiang Li Dandan Yu Huan Ji Baocun Zhao Lili Ji Xiaoping Leng |
| author_facet | Shouqiang Li Dandan Yu Huan Ji Baocun Zhao Lili Ji Xiaoping Leng |
| author_sort | Shouqiang Li |
| collection | DOAJ |
| description | Abstract Background In this paper we aimed to investigate the neovascularization and biodegradation of the silk fibroin in vivo using multiple modes ultrasound, including two-dimensional, three-dimensional and contrast-enhanced ultrasound by quantifying the echo intensity, volume and contrast enhancement of the silk fibroin implants. Method A total of 56 male Wistar rats were randomly divided into two groups and 4%(w/v) silk hydrogels were injected subcutaneously at hind limb or upper back of the rats respectively to compare the biodegradation rate in different sites of the body. The implants were observed at day 0, 4, 8, 12, 16, 18, 20 with multiple modes ultrasound. Results The echo intensity of silk fibroin implants increased and the volume decreased gradually, and complete degradation was confirmed 18 and 20 days after subcutaneous implantation at the upper back and at the hind limb respectively. This demonstrated that the silk fibroin embedded in the upper back degraded slightly faster than that in the hind limb. Additionally, the neovascularization revealed by the contrast enhancement values of CEUS showed that there was a relatively low enhancement (< 5 dB) during day 4 to day 16, followed by moderate enhancement at day 18 (5–20 dB), and a significant enhancement at day 20 (> 40 dB). Conclusion This study suggests that multiple modes ultrasound imaging could be an ideal method to evaluate the degradation and neovascularization of biomaterial implants in vivo for surgical applications. |
| first_indexed | 2024-12-21T14:21:48Z |
| format | Article |
| id | doaj.art-2c482c823a28432abb02fb847ead931c |
| institution | Directory Open Access Journal |
| issn | 1475-925X |
| language | English |
| last_indexed | 2024-12-21T14:21:48Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BioMedical Engineering OnLine |
| spelling | doaj.art-2c482c823a28432abb02fb847ead931c2022-12-21T19:00:45ZengBMCBioMedical Engineering OnLine1475-925X2018-06-0117111410.1186/s12938-018-0478-4In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applicationsShouqiang Li0Dandan Yu1Huan Ji2Baocun Zhao3Lili Ji4Xiaoping Leng5Department of Ultrasound, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Harbin Medical UniversityAbstract Background In this paper we aimed to investigate the neovascularization and biodegradation of the silk fibroin in vivo using multiple modes ultrasound, including two-dimensional, three-dimensional and contrast-enhanced ultrasound by quantifying the echo intensity, volume and contrast enhancement of the silk fibroin implants. Method A total of 56 male Wistar rats were randomly divided into two groups and 4%(w/v) silk hydrogels were injected subcutaneously at hind limb or upper back of the rats respectively to compare the biodegradation rate in different sites of the body. The implants were observed at day 0, 4, 8, 12, 16, 18, 20 with multiple modes ultrasound. Results The echo intensity of silk fibroin implants increased and the volume decreased gradually, and complete degradation was confirmed 18 and 20 days after subcutaneous implantation at the upper back and at the hind limb respectively. This demonstrated that the silk fibroin embedded in the upper back degraded slightly faster than that in the hind limb. Additionally, the neovascularization revealed by the contrast enhancement values of CEUS showed that there was a relatively low enhancement (< 5 dB) during day 4 to day 16, followed by moderate enhancement at day 18 (5–20 dB), and a significant enhancement at day 20 (> 40 dB). Conclusion This study suggests that multiple modes ultrasound imaging could be an ideal method to evaluate the degradation and neovascularization of biomaterial implants in vivo for surgical applications.http://link.springer.com/article/10.1186/s12938-018-0478-4Surgical applicationsSilk fibroinUltrasoundBiodegradationNeovascularization |
| spellingShingle | Shouqiang Li Dandan Yu Huan Ji Baocun Zhao Lili Ji Xiaoping Leng In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications BioMedical Engineering OnLine Surgical applications Silk fibroin Ultrasound Biodegradation Neovascularization |
| title | In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications |
| title_full | In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications |
| title_fullStr | In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications |
| title_full_unstemmed | In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications |
| title_short | In vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications |
| title_sort | in vivo degradation and neovascularization of silk fibroin implants monitored by multiple modes ultrasound for surgical applications |
| topic | Surgical applications Silk fibroin Ultrasound Biodegradation Neovascularization |
| url | http://link.springer.com/article/10.1186/s12938-018-0478-4 |
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